Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,947,850	0	C	T	44.3%	79.9 / 32.9	61	E40K (GAA→AAA)	hdfR	flhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (7/27);  new base T (27/0);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 3.53e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAT  >  NC_000913/3947701‑3948000                                                                                                                                                      |                                                                                                                                                       gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTcc                                                                                                                                                       <  1:315617/151‑1 (MQ=255)      gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTg                                                                                                                                                  <  1:314101/151‑1 (MQ=255)             ggTATGCGCCGCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGAtt                                                                                                                                           <  1:449288/151‑1 (MQ=255)                aTGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGATCCCACCCCGTTGATTTTTCAGTTGTCTg                                                                                                                                              >  1:100230/1‑145 (MQ=255)                  gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGaaa                                                                                                                                      <  2:701070/151‑1 (MQ=255)                  gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGaaa                                                                                                                                      <  1:435785/151‑1 (MQ=255)                  gcgcCACCTCCTTACGGGCGGCCTGCCACGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTTCTTCTGTCGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGaa                                                                                                                                       <  2:755628/150‑1 (MQ=255)                   cgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTTACACCCCGTTGATTTTTCAGTTGTTTTATTTGaaa                                                                                                                                      >  2:602383/1‑150 (MQ=255)                        cctcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCCGTTGATTTTTCCGTTGTCTGATTTGAAAACTCAc                                                                                                                                >  1:489198/1‑151 (MQ=255)                          tcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGTAAAAGGTCACAACCCGTTGATTTTTCCGTTGTTTGATTTTAAAACTCAc                                                                                                                                >  1:6023/1‑149 (MQ=255)                                 gggCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCCGGTGATTTTTCTGTTGTTTGGTTTGAAAAGCCACTGCTGACTg                                                                                                                       >  2:491105/1‑151 (MQ=255)                                  ggcggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGCGAAAAGGTGCACAACCAGGTGGTTTTTCAGTTGTCTGGTTCGAAAGCGCACTGCCGGCTgg                                                                                                                      >  1:334589/1‑151 (MQ=255)                                   gcggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTggg                                                                                                                     <  2:417913/151‑1 (MQ=255)                                     ggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCCGTTGATTTTTCCGTTTTCTGATTCTAAAACTCCCTGCTGGCTGGGt                                                                                                                    >  2:445793/1‑150 (MQ=255)                                           ccAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTCACACCCCAGTGATTTTTCAGTTGTCTGGTTTGAAAAGTCACTGCTGGCTGGGTGAg                                                                                                                 >  2:320749/1‑147 (MQ=255)                                                  cTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCCGTTGATTTTTCCGTTGTCTGATTTGAAAACTCACTGCTGACTGGGTCAGATAAAGCGGtt                                                                                                      >  1:467611/1‑151 (MQ=255)                                                          cGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCCGTTGATTTTTCCCTTGTCTGATTTGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGcc                                                                                                 >  1:496720/1‑148 (MQ=255)                                                           gTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCAGGTGATTTTTCAGTTTTTTGATTTGAAAGCCCACTGCTGACCGGGGCAGATAGAGGGATTTAGcccccc                                                                                             >  1:904654/1‑147 (MQ=255)                                                               cTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCCGGTGTTTTTTCAGCTGTCTGATTCGAAAACTCACTGCTGGCTGGGGCAGATAGAGCGATTTAGCCGCCCGAc                                                                                          >  1:713657/1‑150 (MQ=255)                                                                tGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAGGTTCCCACCCCGTTGATTTTTTCGTTGTCTTATTCGTAAACTCACTGCTGACCGGGTCAGATAGAGGGGTTTAGGCGCTccgcc                                                                                         >  2:337288/1‑146 (MQ=255)                                                                  cATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCAGTTGATTTTTCCGTTGTCTGATTCGAAAACTCACTGCTGACTGGGGCAGATAGAGGGATTCAGCCGCCCGACCaa                                                                                       >  1:238725/1‑150 (MQ=255)                                                                     aaGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCCGTTGGTTTTTCAGTTGTTTTGTTTGAAAACTCACTGCTGACTGGGGCAGATAAAGGGATTTAGCCCCTCCACCcaa                                                                                      >  2:474640/1‑148 (MQ=255)                                                                       ggCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGTGAAAGGGTCACACCCAGTTGATTTTTCCGTTTTCTGTTTATAAAACTCACTGCTGACTGGGTCATGAAGAGCGATTTAGCCGCACGACCCAAATg                                                                                   >  1:543308/1‑149 (MQ=255)                                                                        gCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGATCCCCCCCAGTTGTTTTTTCCGTTTTCTGATTCCAAAACCCACAGCTGCCTGGGTCGGAGAGCACGATTCAGCCCCTCGaacca                                                                                       >  1:251266/1‑140 (MQ=255)                                                                             agtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTc                                                                           <  2:176431/151‑1 (MQ=255)                                                                              gtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCg                                                                          <  1:212730/151‑1 (MQ=255)                                                                                 tttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGc                                                                        <  1:467881/150‑1 (MQ=255)                                                                                  ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCt                                                                       <  2:861152/150‑1 (MQ=255)                                                                                  ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCt                                                                       <  2:671421/150‑1 (MQ=255)                                                                                  ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGGTTACACCCCGTTTATTTTTCCGTTGTCTGATTTGAAAGCTCACTGCTGGCTGGGGCAGATAGAGCGATTCAGCCGCTCCACCCAAAAGAcgcggt                                                                            >  1:482696/1‑143 (MQ=255)                                                                                     aCCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGGTCACACCCAGGTGATTTTTCAGTTGTCTGGTTCGAAAACTCACTGGTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGCCCAAAAAGAAGCGGTCGGCCaa                                                                     >  2:47390/1‑149 (MQ=255)                                                                                     aCCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTCACACCCAGTTGATTTTTCAGTTGTCTGATTCGAAAACTCACTGCTGACTGGGGCAGATAGAGCGATTCAGCCGCCCGACCAAAGTGACGCGTTCGGCTAAc                                                                    >  1:669320/1‑150 (MQ=255)                                                                                     aCCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCCGTTGATTTTTCCGTTGTTTGGTTTGAAAACTCCCTGCTGGCTGGGGGAGATAGAGCGGTTTAGCCGCCCGGCCCAAGTGACCCGGTCGGGTaa                                                                     >  2:156740/1‑149 (MQ=255)                                                                                              ggTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAACTCACTGCTGACTGGGGCAGATAGAGCGATTCAGCCGCCCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGaa                                                           >  1:108578/1‑150 (MQ=255)                                                                                               gTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGGTCACACCCAGTTGATTTTTCCGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGGCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGGTTGGCTAACTTCCAGGaa                                                           >  1:820841/1‑149 (MQ=255)                                                                                                ttAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCCCACCCCGGTGATTTTTTACTTGTTTGGTTTGAAAAATCACTGCTGAATGGGGCAGATAGAGGGGTTTAGCCGCTCGACCAAAAAGACGGGTTCGGCTAACTTCCAGGaa                                                           >  2:66988/1‑148 (MQ=255)                                                                                                  aaaCGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGttt                                                      <  1:841299/151‑1 (MQ=255)                                                                                                  aaaCGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGtt                                                       <  1:469502/150‑1 (MQ=255)                                                                                                    aCGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGttttt                                                    <  2:866492/151‑1 (MQ=255)                                                                                                    aCGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCCCACCCAGTTGATTTTTCAGTTTTTTGGTTTGAAAACTCACTGCTGACTGGGGCAGATAGAGGGATTTAGCCGCCCGACCAAAGTGACGCGGTTGGGTAACTTCCAGGGAAGatttt                                                    >  2:326320/1‑151 (MQ=255)                                                                                                     cGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTa                                                   <  1:493303/151‑1 (MQ=255)                                                                                                     cGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCAGGTGATTTTTCAGTTGTCTGGTTTGGAAAATCACTGCTGGCTGGGGCAGATAGAGCGATTTAGCCGCCCGACCAAAAGGAAGCGGTCGGCTTACTTCCAGGAAAGgtttt                                                    >  2:458300/1‑150 (MQ=255)                                                                                                          atTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACaa                                               <  1:352031/150‑1 (MQ=255)                                                                                                            tGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAAtt                                             <  2:24096/150‑1 (MQ=255)                                                                                                               ttCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCg                                          <  1:99821/150‑1 (MQ=255)                                                                                                                tCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGt                                         <  2:810913/150‑1 (MQ=255)                                                                                                                   gtgGCGGGTGAAAAGGTTCACACCCAGTTGATTTTTCAGTTGTCTGGTTCGCAAACTCACTGCCGACTGGGTCAGATAGAGCGATTTAGACGCTCGACCAAAGTGACGCGTTCGGCGAACATCCAGGAAAGTTTTTAAAAAtttc                                           >  2:686650/1‑143 (MQ=255)                                                                                                                   gtgGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATc                                      <  2:221213/150‑1 (MQ=255)                                                                                                                   gtgGCGGGTGAAAAAGGTCACACCCAGGTGGTTTTTCCGTTGTTTGATTCGAAAACTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAAGGACGCGGTCGGCTAACTTCCAGGAAAGTTTTTAACAATTTCGTATcc                                     >  2:567565/1‑151 (MQ=255)                                                                                                                     ggCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcac                                   >  2:516730/1‑151 (MQ=255)                                                                                                                      gCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcaca                                  <  2:632603/151‑1 (MQ=255)                                                                                                                              aaaGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCtctc                          <  1:241370/151‑1 (MQ=255)                                                                                                                                aGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTcca                        <  1:248658/151‑2 (MQ=255)                                                                                                                                 ggTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGc                       >  2:317372/1‑151 (MQ=255)                                                                                                                                       cacCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAat                  <  2:100229/150‑1 (MQ=255)                                                                                                                                          ccAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAattatt              <  2:668520/151‑1 (MQ=255)                                                                                                                                           cAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTAttt             <  2:3061/151‑1 (MQ=255)                                                                                                                                           cAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTAttt             >  1:229591/1‑151 (MQ=255)                                                                                                                                           cAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTAttt             <  1:716774/151‑1 (MQ=255)                                                                                                                                                  ttttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGtt       >  1:29713/1‑150 (MQ=255)                                                                                                                                                   tttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTAt     >  2:778805/1‑151 (MQ=255)                                                                                                                                                   tttCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGCACAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCAACAGTCATCTCCGCAACCTTATTTGTCGTTAt     >  2:629554/1‑151 (MQ=255)                                                                                                                                                      ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAt  <  1:662246/151‑1 (MQ=255)                                                                                                                                                      ccAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAt  <  2:225394/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGCAAAATTATTTGTCGTTATGAT  >  NC_000913/3947701‑3948000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑