Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	38,511	T→G	E202A (GAA→GCA)	caiB ←	crotonobetainyl CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	38,511	0	T	G	100.0%	190.5 / NA	50	E202A (GAA→GCA)	caiB	crotonobetainyl CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/23);  total (27/23)

CACCAGTTCCATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTC  >  NC_000913/38363‑38657                                                                                                                                                     |                                                                                                                                                   caccaGTTCCATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCa                                                                                                                                                  <  2:136440/151‑1 (MQ=255)          cATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCg                                                                                                                                         <  2:567951/151‑1 (MQ=255)           aTCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCg                                                                                                                                         >  2:227661/1‑150 (MQ=255)           aTCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGa                                                                                                                                        <  2:114540/151‑1 (MQ=255)                gATGTAGCCGTCTGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGAAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTc                                                                                                                                    >  2:587741/1‑150 (MQ=255)                  tGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATAATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGAt                                                                                                                                 >  2:68662/1‑151 (MQ=255)                        cGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTc                                                                                                                           <  2:715186/151‑1 (MQ=255)                        cGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTc                                                                                                                           <  1:527480/151‑1 (MQ=255)                           cGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGc                                                                                                                         <  2:883141/150‑1 (MQ=255)                              cacaTTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCttt                                                                                                                     <  2:190007/151‑1 (MQ=255)                               acaTTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCttt                                                                                                                     >  1:297917/1‑150 (MQ=255)                                caTTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTAc                                                                                                                   >  1:444503/1‑151 (MQ=255)                                 aTTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTAcc                                                                                                                  <  1:661799/151‑1 (MQ=255)                                  tttATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCg                                                                                                                 >  2:615609/1‑151 (MQ=255)                                  tttATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCg                                                                                                                 >  1:742262/1‑151 (MQ=255)                                     ataCAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGt                                                                                                               <  1:808282/150‑1 (MQ=255)                                       aCAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTc                                                                                                            >  2:359916/1‑151 (MQ=255)                                       aCAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTc                                                                                                            <  2:793157/151‑1 (MQ=255)                                             cGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCAc                                                                                                      >  1:363499/1‑151 (MQ=255)                                             cGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCAc                                                                                                      <  2:123406/151‑1 (MQ=255)                                                aaCCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACgtt                                                                                                   >  2:83554/1‑151 (MQ=255)                                                  ccGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTAt                                                                                                 >  1:483950/1‑151 (MQ=255)                                                      cGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGc                                                                                               >  1:450331/1‑149 (MQ=255)                                                      cGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGc                                                                                               >  1:245577/1‑149 (MQ=255)                                                        tagtagGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGtg                                                                                           >  1:864593/1‑151 (MQ=255)                                                              ggATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCa                                                                                     <  2:366470/151‑1 (MQ=255)                                                                  cTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCACACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAgc                                                                                   >  1:326966/1‑149 (MQ=255)                                                                   tttACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGcgcc                                                                                >  1:774061/1‑151 (MQ=255)                                                                      aCCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCgccgcc                                                                             >  2:419520/1‑151 (MQ=255)                                                                          ttGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCgt                                                                           <  2:471417/149‑1 (MQ=255)                                                                            gCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCgtggtg                                                                       <  1:362885/151‑1 (MQ=255)                                                                                aTGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCgg                                                                   >  2:689402/1‑151 (MQ=255)                                                                                  gcgcGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGt                                                                  <  2:90999/150‑1 (MQ=255)                                                                                    gcgGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAg                                                               <  2:882115/151‑1 (MQ=255)                                                                                           caTTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGaa                                                        <  2:426329/151‑1 (MQ=255)                                                                                           caTTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGa                                                         <  2:181067/150‑1 (MQ=255)                                                                                                  ccgccgTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAAt                                                 <  1:148182/151‑1 (MQ=255)                                                                                                                    atcatGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAagg                               >  1:397464/1‑151 (MQ=255)                                                                                                                      catGAAGTACTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAaggc                              >  2:197510/1‑150 (MQ=255)                                                                                                                            gcaCTGGCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTAAACGGGAAggcaggca                         >  2:797828/3‑149 (MQ=255)                                                                                                                                 ggCCCATACGCAGCATCTCTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGc                    <  2:862360/149‑1 (MQ=255)                                                                                                                                 ggCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCt                   <  1:43672/150‑1 (MQ=255)                                                                                                                                 ggCCCATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTg                  <  1:292330/151‑1 (MQ=255)                                                                                                                                    ccATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTc               <  2:409079/151‑1 (MQ=255)                                                                                                                                     cATACGCAGCATCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTc               >  1:69221/1‑150 (MQ=255)                                                                                                                                          gcagcaTCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCaaattc         >  2:740695/1‑147 (MQ=255)                                                                                                                                          gcagcaTCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACAt          >  2:70674/1‑150 (MQ=255)                                                                                                                                          gcagcaTCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACAt          >  2:195297/1‑150 (MQ=255)                                                                                                                                                tCACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGtt   >  1:817450/1‑151 (MQ=255)                                                                                                                                                 cACTGCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTc  >  2:875557/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CACCAGTTCCATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTC  >  NC_000913/38363‑38657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑