Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,521,793	T→G	N229H (AAT→CAT)	xapR ←	transcriptional activator of xapAB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,521,793	0	T	G	100.0%	309.0 / NA	78	N229H (AAT→CAT)	xapR	transcriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/39);  total (39/39)

TCATAAACAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATG  >  NC_000913/2521648‑2521940                                                                                                                                                  |                                                                                                                                                    tCATAAACAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACt                                                                                                                                                 <  1:626650/150‑1 (MQ=255) tCATAAACAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACt                                                                                                                                                 <  2:716650/150‑1 (MQ=255)   aTAAACAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTcg                                                                                                                                              >  1:612469/1‑151 (MQ=255)      aaCAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTcgcg                                                                                                                                            >  2:625179/1‑150 (MQ=255)      aaCAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTcgcg                                                                                                                                            >  2:410667/1‑150 (MQ=255)        cAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAAt                                                                                                                                         >  1:471027/1‑151 (MQ=255)              ataAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATaacaac                                                                                                                                   <  2:14827/151‑1 (MQ=255)                aaaTCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAAcc                                                                                                                                  <  1:903244/150‑1 (MQ=255)                      gCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGt                                                                                                                               <  1:162444/147‑1 (MQ=255)                      gCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGaa                                                                                                                            >  1:714564/1‑150 (MQ=255)                       cAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGaaa                                                                                                                           >  1:663259/1‑150 (MQ=255)                          ggATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATcc                                                                                                                       <  2:50039/151‑1 (MQ=255)                              gcgcTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCAc                                                                                                                    <  2:30324/150‑1 (MQ=255)                                  tGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACctgct                                                                                                               >  2:659541/1‑151 (MQ=255)                                  tGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACctgct                                                                                                               >  1:13505/1‑151 (MQ=255)                                   gTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACctgctg                                                                                                              >  2:628979/1‑151 (MQ=255)                                     ttGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTgg                                                                                                             <  2:149444/150‑1 (MQ=255)                                       gAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCaa                                                                                                          <  2:858176/151‑1 (MQ=255)                                       gAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCaa                                                                                                          <  1:655511/151‑1 (MQ=255)                                        aGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCaaa                                                                                                         <  2:207057/151‑1 (MQ=255)                                         gCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAAc                                                                                                        <  2:585648/151‑1 (MQ=255)                                          cggacggaAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACt                                                                                                       <  1:190543/151‑1 (MQ=255)                                           ggacggaAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTc                                                                                                      >  2:526276/1‑151 (MQ=255)                                            gacggaAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCg                                                                                                     >  1:835986/1‑151 (MQ=255)                                             acggaAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGc                                                                                                    >  2:55135/1‑151 (MQ=255)                                             acggaAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGc                                                                                                    >  2:494482/1‑151 (MQ=255)                                               ggaAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTg                                                                                                  <  1:63170/151‑1 (MQ=255)                                                 aaaaTGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGc                                                                                                 >  2:777108/1‑150 (MQ=255)                                                   aaTGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCaa                                                                                               >  1:871103/1‑150 (MQ=255)                                                      gACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCaaaaaa                                                                                           <  1:494095/151‑1 (MQ=255)                                                      gACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCaaaaa                                                                                            <  1:159397/150‑1 (MQ=255)                                                         acCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATcc                                                                                        >  1:113957/1‑151 (MQ=255)                                                         acCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATcc                                                                                        >  1:738287/1‑151 (MQ=255)                                                             ggCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCcagt                                                                                    >  2:641766/1‑151 (MQ=255)                                                               ccAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCcagtca                                                                                  <  2:506296/151‑1 (MQ=255)                                                                cAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGTGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCcagtc                                                                                   >  2:326548/1‑149 (MQ=255)                                                                cAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCcagtc                                                                                   >  2:532069/1‑149 (MQ=255)                                                                cAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCcagtc                                                                                   >  2:726210/1‑149 (MQ=255)                                                                  gTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAgtg                                                                               <  2:281499/151‑1 (MQ=255)                                                                      aTTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTaa                                                                            >  2:747573/1‑150 (MQ=255)                                                                            gCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGggc                                                                      <  2:113469/150‑1 (MQ=255)                                                                             cGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGggcgg                                                                    >  1:406892/1‑151 (MQ=255)                                                                                   tGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGt                                                              >  1:803265/1‑151 (MQ=255)                                                                                    gTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGt                                                              <  2:329590/150‑1 (MQ=255)                                                                                     tCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTa                                                             >  1:24138/1‑150 (MQ=255)                                                                                      ccGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAAc                                                           >  1:495618/1‑151 (MQ=255)                                                                                         cTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACaa                                                         >  2:281814/1‑150 (MQ=255)                                                                                          tATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACaaa                                                        >  1:781664/1‑150 (MQ=255)                                                                                           aTCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACaaaa                                                       <  2:503110/150‑1 (MQ=255)                                                                                               aTGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTc                                                  >  1:648479/1‑151 (MQ=255)                                                                                               aTGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTc                                                  >  2:205019/1‑151 (MQ=255)                                                                                                 gtgATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCg                                                 <  1:584391/150‑1 (MQ=255)                                                                                                   gATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTc                                               <  2:212143/150‑1 (MQ=255)                                                                                                    aTACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCAc                                             >  1:551050/1‑151 (MQ=255)                                                                                                      aCCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGt                                           <  2:889396/151‑1 (MQ=255)                                                                                                            gCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCt                                     <  2:406883/151‑1 (MQ=255)                                                                                                              ccATACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTc                                   >  2:539263/1‑151 (MQ=255)                                                                                                                aTACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTcc                                  >  1:772200/1‑150 (MQ=255)                                                                                                                aTACTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCa                                 <  2:481996/151‑1 (MQ=255)                                                                                                                  aCTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAg                                <  1:220072/150‑1 (MQ=255)                                                                                                                  aCTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAg                                <  2:550074/150‑1 (MQ=255)                                                                                                                  aCTGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAg                                <  2:381705/150‑1 (MQ=255)                                                                                                                    tGACCATAGCGAGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCgg                             >  2:136915/1‑151 (MQ=255)                                                                                                                               aGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGaaa                  <  1:641781/151‑1 (MQ=255)                                                                                                                               aGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGaaa                  <  2:678933/151‑1 (MQ=255)                                                                                                                               aGTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGaaa                  <  1:519205/151‑1 (MQ=255)                                                                                                                                gTACCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGaaaa                 <  2:581474/151‑1 (MQ=255)                                                                                                                                  aCCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAatg               >  1:841476/1‑151 (MQ=255)                                                                                                                                  aCCGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAat                <  2:198039/150‑1 (MQ=255)                                                                                                                                   ccGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAatg               <  2:615610/150‑1 (MQ=255)                                                                                                                                   ccGTTTGCGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAatg               >  1:761680/1‑150 (MQ=255)                                                                                                                                          cGGTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATgag          >  2:131058/1‑148 (MQ=255)                                                                                                                                           gggtCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATgag          >  2:439165/4‑147 (MQ=255)                                                                                                                                           ggTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAgat      <  2:543802/151‑1 (MQ=255)                                                                                                                                            gTTCATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAgatg     <  1:10615/151‑1 (MQ=255)                                                                                                                                               cATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAgatgat   <  1:718029/150‑1 (MQ=255)                                                                                                                                               cATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGatgatg  <  1:916934/151‑1 (MQ=255)                                                                                                                                               cATGAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGatgatg  >  1:865170/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TCATAAACAATATATAAATCTGCAGGGATGCGCTGTTTGAGCGGACGGAAAATGACACCTGGCCAGTTCATTTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATG  >  NC_000913/2521648‑2521940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑