Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,546,546 T→G I258M (ATT→ATG murQ → N‑acetylmuramic acid 6‑phosphate (MurNAc‑6‑P) etherase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,546,5460TG100.0% 214.7 / NA 56I258M (ATT→ATGmurQN‑acetylmuramic acid 6‑phosphate (MurNAc‑6‑P) etherase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/28);  total (28/28)

ACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCA  >  NC_000913/2546400‑2546691
                                                                                                                                                  |                                                                                                                                                  
aCCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAAtggctt                                                                                                                                                >  2:178178/1‑151 (MQ=255)
         atgatTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAgcgc                                                                                                                                        >  1:273689/1‑150 (MQ=255)
           gatTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAAc                                                                                                                                     >  1:866284/1‑151 (MQ=255)
               aaaTCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGt                                                                                                                                  >  2:411548/1‑150 (MQ=255)
                 aTCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTaa                                                                                                                                >  2:200704/1‑150 (MQ=255)
                        aaaGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGcc                                                                                                                         >  1:894706/1‑150 (MQ=255)
                          aGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCAtt                                                                                                                      >  1:503638/1‑151 (MQ=255)
                                cGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATg                                                                                                                <  1:179646/151‑1 (MQ=255)
                                   cAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTg                                                                                                             <  2:106415/151‑1 (MQ=255)
                                    aaCCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGc                                                                                                            >  2:907684/1‑151 (MQ=255)
                                     aCCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGc                                                                                                            >  1:261406/1‑150 (MQ=255)
                                         gATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGaa                                                                                                        >  2:783732/1‑150 (MQ=255)
                                            ggTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGaaaaa                                                                                                     <  2:735593/150‑1 (MQ=255)
                                               cGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAAtctc                                                                                                 <  2:840331/151‑1 (MQ=255)
                                               cGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAAtct                                                                                                  <  2:702275/150‑1 (MQ=255)
                                                gATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGAGCAACTGTAAAACGGCTATTGTGATGGTGCTGAAAAAtctc                                                                                                 >  2:745652/1‑150 (MQ=255)
                                                     ggTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGcc                                                                                          >  1:519963/1‑151 (MQ=255)
                                                          ccaccaACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGc                                                                                        >  2:780481/1‑148 (MQ=255)
                                                          ccaccaACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGc                                                                                        >  1:766648/1‑148 (MQ=255)
                                                          ccaccaACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGa                                                                                     <  1:80699/151‑1 (MQ=255)
                                                           caccaACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGa                                                                                     >  2:249706/1‑150 (MQ=255)
                                                           caccaACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGa                                                                                     >  2:887510/1‑150 (MQ=255)
                                                            accaACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGa                                                                                     >  2:536268/1‑149 (MQ=255)
                                                            accaACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAg                                                                                   <  1:249767/151‑1 (MQ=255)
                                                               aaCGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTa                                                                                <  1:906262/151‑1 (MQ=255)
                                                                      aaCTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACg                                                                         >  1:882255/1‑151 (MQ=255)
                                                                       aCTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGTATGCCgtcttcctattgaa                                                                            >  1:260989/1‑135 (MQ=255)
                                                                          gCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGcc                                                                       <  1:878902/149‑1 (MQ=255)
                                                                          gCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGcc                                                                       <  1:739216/149‑1 (MQ=255)
                                                                          gCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGcc                                                                       <  2:351131/149‑1 (MQ=255)
                                                                            aTGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTgg                                                                    >  1:389939/1‑150 (MQ=255)
                                                                                gCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCaa                                                               <  1:352403/151‑1 (MQ=255)
                                                                                     aGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACAcgg                                                          >  1:361613/1‑151 (MQ=255)
                                                                                       gTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACAcggcg                                                        >  1:733174/1‑151 (MQ=255)
                                                                                       gTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACAcggcg                                                        <  1:636259/151‑1 (MQ=255)
                                                                                           atatTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCtt                                                    <  2:866265/151‑1 (MQ=255)
                                                                                            tatTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCttt                                                   >  2:924256/1‑151 (MQ=255)
                                                                                             atTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCttt                                                   <  2:608190/150‑1 (MQ=255)
                                                                                             atTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCttt                                                   <  2:337156/150‑1 (MQ=255)
                                                                                             atTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCttt                                                   >  1:402816/1‑150 (MQ=255)
                                                                                                gTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTAtt                                                <  1:66058/150‑1 (MQ=255)
                                                                                                  tAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCg                                              <  1:187527/150‑1 (MQ=255)
                                                                                                   aaaaaCGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGt                                             <  2:56051/150‑1 (MQ=255)
                                                                                                       aCGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGt                                        >  2:349811/1‑151 (MQ=255)
                                                                                                        cGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGGGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGtt                                       >  1:565304/1‑151 (MQ=255)
                                                                                                         gCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGttt                                      >  1:880768/1‑151 (MQ=255)
                                                                                                         gCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGttt                                      <  1:725665/151‑1 (MQ=255)
                                                                                                           cACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTa                                    <  2:579966/151‑1 (MQ=255)
                                                                                                                   gTAGCGCAGAGCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGa                            <  1:748532/151‑1 (MQ=255)
                                                                                                                             gCAAGCGGAAGCGGCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCAt                   <  2:759302/150‑1 (MQ=255)
                                                                                                                                   ggaagcggCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACCCGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCa              <  1:370573/149‑1 (MQ=255)
                                                                                                                                   ggaagcggCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCaaa            <  1:537107/151‑1 (MQ=255)
                                                                                                                                    gaagcggCGTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCaa             >  2:743433/1‑149 (MQ=255)
                                                                                                                                        cggcgTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAag           >  2:432687/1‑147 (MQ=255)
                                                                                                                                          gcgTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATca     <  2:351236/151‑1 (MQ=255)
                                                                                                                                          gcgTTAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATca     <  2:609475/151‑1 (MQ=255)
                                                                                                                                              tAATGGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATcagca  <  2:708708/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCA  >  NC_000913/2546400‑2546691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: