Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,593,811	A→C	intergenic (‑41/+33)	ypfH ← / ← tmcA	palmitoyl‑CoA esterase activity, uncertain physiological substrate/elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,593,811	0	A	C	100.0%	265.4 / NA	70	intergenic (‑41/+33)	ypfH/tmcA	palmitoyl‑CoA esterase activity, uncertain physiological substrate/elongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/42);  total (28/42)

CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTT  >  NC_000913/2593671‑2593957                                                                                                                                             |                                                                                                                                                   cccccATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGa                                                                                                                                          <  1:131211/151‑1 (MQ=255) cccccATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGa                                                                                                                                          <  2:666157/151‑1 (MQ=255)    ccATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCa                                                                                                                                       <  2:63100/151‑1 (MQ=255)    ccATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCa                                                                                                                                       <  2:189023/151‑1 (MQ=255)     cATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCAt                                                                                                                                      >  2:514447/1‑151 (MQ=255)     cATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCAt                                                                                                                                      <  2:525621/151‑1 (MQ=255)        tGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATga                                                                                                                                    <  2:510444/150‑1 (MQ=255)          ccACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGAt                                                                                                                                 <  1:207414/151‑1 (MQ=255)            aCCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATaa                                                                                                                               <  1:79170/151‑1 (MQ=255)             ccGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATaa                                                                                                                               >  2:22645/1‑150 (MQ=255)              cGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATaaa                                                                                                                              >  2:785823/1‑150 (MQ=255)                 gTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTg                                                                                                                          <  1:114772/151‑1 (MQ=255)                   tATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGa                                                                                                                         >  2:294421/1‑150 (MQ=255)                     tCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAg                                                                                                                       <  2:371754/150‑1 (MQ=255)                      ccccGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAgg                                                                                                                      <  2:881274/150‑1 (MQ=255)                                  ggAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAaat                                                                                                          >  2:418719/1‑150 (MQ=255)                                   gAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAaataa                                                                                                        <  1:153675/151‑1 (MQ=255)                                     aaaGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTGACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCGGTGAAATAAtt                                                                                                      <  1:83576/151‑1 (MQ=255)                                     aaaGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAAtt                                                                                                      <  1:47073/151‑1 (MQ=255)                                      aaGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTg                                                                                                     >  1:714961/1‑151 (MQ=255)                                             aacaacTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTg                                                                                               <  1:811635/150‑1 (MQ=255)                                                    gTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATg                                                                                        <  2:208686/150‑1 (MQ=255)                                                          cAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATcgc                                                                                 <  2:847878/151‑1 (MQ=255)                                                              tttATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCgcgc                                                                               >  1:767763/1‑149 (MQ=255)                                                                 aTCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACg                                                                          <  2:598811/151‑1 (MQ=255)                                                                  tCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGc                                                                         >  1:75778/1‑151 (MQ=255)                                                                     gggCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTc                                                                       >  2:116413/1‑150 (MQ=255)                                                                       gCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGt                                                                    >  2:341071/1‑151 (MQ=255)                                                                        cTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTg                                                                   >  1:884952/1‑151 (MQ=255)                                                                          ttGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTg                                                                   <  1:800778/149‑1 (MQ=255)                                                                          ttGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGc                                                                  <  2:779805/150‑1 (MQ=255)                                                                          ttGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGc                                                                  <  2:771620/150‑1 (MQ=255)                                                                          ttGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGc                                                                  <  2:624384/150‑1 (MQ=255)                                                                             aacaacAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAAc                                                              <  2:175012/151‑1 (MQ=255)                                                                              acaacaAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAAca                                                             <  2:921161/151‑1 (MQ=255)                                                                               caacaaAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAAcat                                                            <  2:445324/151‑1 (MQ=255)                                                                                aacaaAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAAcatc                                                           >  1:459038/1‑151 (MQ=255)                                                                                aacaaAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAAcatc                                                           <  1:514461/151‑1 (MQ=255)                                                                                   aaaaTGGTCATGTTTCATGGCGAACTCCTTAACTGATGACATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAAcatca                                                          >  1:344334/1‑149 (MQ=255)                                                                                     aaTGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTa                                                       >  1:313755/1‑150 (MQ=255)                                                                                      aTGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTa                                                       >  2:237246/1‑149 (MQ=255)                                                                                      aTGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAg                                                     <  1:418728/151‑1 (MQ=255)                                                                                         gTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGc                                                  <  1:796789/151‑1 (MQ=255)                                                                                            aTGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGa                                                >  1:29319/1‑150 (MQ=255)                                                                                              gTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACa                                             <  1:601882/151‑1 (MQ=255)                                                                                               tttCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAAc                                              >  1:277718/1‑149 (MQ=255)                                                                                                 tCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAgc                                           <  1:797817/150‑1 (MQ=255)                                                                                                     ggCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTg                                      >  2:542008/1‑151 (MQ=255)                                                                                                        gAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCGTTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTgcgc                                   <  1:727343/151‑1 (MQ=255)                                                                                                             ccTTAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGcc                               <  1:240321/150‑1 (MQ=255)                                                                                                                tAACTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCctc                             <  1:345535/149‑1 (MQ=255)                                                                                                                 aaCTGATGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCtt                           <  1:629689/150‑1 (MQ=255)                                                                                                                      aTGTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCt                     <  1:365735/151‑1 (MQ=255)                                                                                                                        gTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTg                    >  1:486750/1‑150 (MQ=255)                                                                                                                        gTCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTg                    <  2:693324/150‑1 (MQ=255)                                                                                                                         tCATTAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGAc                  >  1:589639/1‑151 (MQ=255)                                                                                                                            ttAATTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACgg                >  1:687125/1‑150 (MQ=255)                                                                                                                              aaTTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGAc              <  1:244530/150‑1 (MQ=255)                                                                                                                               aTTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGAcc             >  2:697273/1‑150 (MQ=255)                                                                                                                               aTTTTACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCa            <  2:767638/151‑1 (MQ=255)                                                                                                                                  ttACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGt          <  1:876167/150‑1 (MQ=255)                                                                                                                                   tACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa         >  2:463163/1‑150 (MQ=255)                                                                                                                                   tACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa         <  1:760524/150‑1 (MQ=255)                                                                                                                                   tACGCCGCTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa         >  2:814068/1‑150 (MQ=255)                                                                                                                                     cgccgcTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACa      <  1:339567/151‑1 (MQ=255)                                                                                                                                      gccgcTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACAt     >  2:23758/1‑151 (MQ=255)                                                                                                                                       ccgcTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATc    >  1:918629/1‑151 (MQ=255)                                                                                                                                        cgcTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCt   <  2:714033/151‑1 (MQ=255)                                                                                                                                         gcTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCtt  >  1:468399/1‑151 (MQ=255)                                                                                                                                         gcTGCCTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCtt  >  1:328928/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                   CCCCCATTGCCACCGGGTTATCCCCGACACCGTGGAAAAGCAGTAACAACTGTTGCGCAGGTTTATCCGGGCTTTGAACAACAAAATGGTCATGTTTCATGGCGAACTCCTTAACTGATGTCATTAATTTTACGCCGCTGACTCACAATGACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTT  >  NC_000913/2593671‑2593957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑