Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,615,642	T→G	I80I (ATA→ATC)	yfgO ←	putative UPF0118 family inner membrane permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,615,642	0	T	G	100.0%	199.2 / NA	52	I80I (ATA→ATC)	yfgO	putative UPF0118 family inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/23);  total (29/23)

AATGCCCGCGTCCATTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAG  >  NC_000913/2615495‑2615786                                                                                                                                                    |                                                                                                                                                 aatGCCCGCGTCCATTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAagat                                                                                                                                                >  2:216042/1‑150 (MQ=255)   tGCCCGCGTCCATTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATAcc                                                                                                                                             >  2:302365/1‑151 (MQ=255)    gCCCGCGTCCATTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACcg                                                                                                                                            <  1:154648/151‑1 (MQ=255)       cgcgTCCATTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACcgacg                                                                                                                                         >  1:800548/1‑151 (MQ=255)            ccATTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAac                                                                                                                                    >  1:447344/1‑151 (MQ=255)              aTTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAacca                                                                                                                                  <  1:695553/151‑1 (MQ=255)               ttAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAaccac                                                                                                                                 >  1:116516/1‑151 (MQ=255)                   cGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCaa                                                                                                                              <  2:723808/150‑1 (MQ=255)                    gCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAAt                                                                                                                             >  1:688043/1‑150 (MQ=255)                      cGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATAc                                                                                                                           >  1:456208/1‑150 (MQ=255)                          tAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCg                                                                                                                      >  1:797210/1‑151 (MQ=255)                                cgcggcAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCg                                                                                                                >  2:357856/1‑151 (MQ=255)                                  cggcAACGAGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGcc                                                                                                              >  2:216706/1‑151 (MQ=255)                                     cAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGccc                                                                                                             >  2:357247/1‑149 (MQ=255)                                      aaCGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAgc                                                                                                          <  1:669722/151‑1 (MQ=255)                                         gTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAgcggc                                                                                                       <  2:104417/151‑1 (MQ=255)                                                cAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAgcggcg                                                                                                      >  2:193534/1‑145 (MQ=255)                                                cAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGag                                                                                                  >  2:672527/1‑149 (MQ=255)                                                      cAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGcc                                                                                             >  1:824780/1‑148 (MQ=255)                                                          aaGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGcc                                                                                             >  1:799632/1‑144 (MQ=255)                                                            gCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGGGCAGCCCATAAAtt                                                                                     >  1:705406/1‑150 (MQ=255)                                                             cTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTATCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAAAAGATTg                                                                                    >  2:863953/1‑150 (MQ=255)                                                              ttATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGc                                                                                   >  2:420895/1‑150 (MQ=255)                                                               tATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCa                                                                                  <  1:589145/150‑1 (MQ=255)                                                                  tGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGAc                                                                               >  1:337602/1‑150 (MQ=255)                                                                     gCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCAc                                                                           <  2:171823/151‑1 (MQ=255)                                                                       aTCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACgg                                                                         <  2:259651/151‑1 (MQ=255)                                                                         ccccGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGtt                                                                       <  1:349273/151‑1 (MQ=255)                                                                              gCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCa                                                                  <  2:364086/151‑1 (MQ=255)                                                                                  atCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTcc                                                              >  1:751691/1‑151 (MQ=255)                                                                                    cgcgGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTcc                                                              >  1:357844/1‑149 (MQ=255)                                                                                     gcgGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCCGCCAAAAGATTGCAGACGCACGGTTGGCCCTTCCAGc                                                           >  1:785835/1‑151 (MQ=255)                                                                                     gcgGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAg                                                            >  1:169479/1‑150 (MQ=255)                                                                                     gcgGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGc                                                           <  1:845941/151‑1 (MQ=255)                                                                                       ggATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGc                                                           >  1:279372/1‑149 (MQ=255)                                                                                           tAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGAATGCAAACGCACGGTTGGCCATTCCAGCaaa                                                        >  1:428880/1‑148 (MQ=255)                                                                                                aGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGGTGGCCCTTCCAGCAAAAAGGcc                                                  >  2:295876/1‑149 (MQ=255)                                                                                                  aTGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGca                                              <  2:236539/151‑1 (MQ=255)                                                                                                  aTGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGca                                              <  2:228260/151‑1 (MQ=255)                                                                                                         gTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGCCGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAg                                        <  1:217109/150‑1 (MQ=255)                                                                                                          ttGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAAAAAATTGCAGACGAACGGGTGGCCATTTCAGGAAAAAGGCCAGGACaaa                                          >  2:725187/1‑146 (MQ=255)                                                                                                            gCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGcca                                     <  1:64964/150‑1 (MQ=255)                                                                                                              cAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAGAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAACCACAAGATTGCAGACGCACGGCTGGCCCTCCCCTCAAAAAGGCCCGCCCAAAAGcc                                      >  2:414303/1‑147 (MQ=255)                                                                                                              cAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGGTGGCCATTCCAGCAAATAGGCCAGCACAAAAGccacc                                   >  1:446640/1‑150 (MQ=255)                                                                                                                          aGTACCACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGagcaa                      <  2:456196/151‑1 (MQ=255)                                                                                                                              ccACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGagcaagca                   <  1:403097/150‑1 (MQ=255)                                                                                                                              ccACGAACGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAg                  <  1:193372/151‑1 (MQ=255)                                                                                                                                   aaCGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGGAGCCAAAAGAATGCAGACCCACGGTTGGCCATTCCAGGAAATAAGCCAGCACAATAGACACCAGCAAAGGGGCAAGCAGGCCAc             >  1:276519/1‑151 (MQ=255)                                                                                                                                   aaCGCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCAc             <  1:16002/151‑1 (MQ=255)                                                                                                                                      gCCATCAGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTaa          <  1:672543/151‑1 (MQ=255)                                                                                                                                         aTCAATAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAg        <  2:540535/150‑1 (MQ=255)                                                                                                                                            aGTAGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGaa      <  1:334520/149‑1 (MQ=255)                                                                                                                                               aGCAAGATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAg  <  2:797192/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 AATGCCCGCGTCCATTAACGCCGGATAGCGGCGCGGCAACGTGGCGGCAAAGTCAGAAAGCTTATTGAGCATCCCCGGCATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAG  >  NC_000913/2615495‑2615786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑