Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,648,982	A→C	I1102M (ATT→ATG)	yfhM ←	bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,648,982	0	A	C	100.0%	196.4 / NA	52	I1102M (ATT→ATG)	yfhM	bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/27);  total (25/27)

AATCCATCCGCCGGAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTAATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGCGGTCAGGGCA  >  NC_000913/2648836‑2649123                                                                                                                                                   |                                                                                                                                              aaTCCATCCGCCGGAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCAtgg                                                                                                                                           >  1:639781/1‑151 (MQ=255)    ccatccGCCGGAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCAtggtgg                                                                                                                                        >  2:903479/1‑151 (MQ=255)         cgccgGAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGa                                                                                                                                   <  2:516995/151‑1 (MQ=255)            cgGAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAAtt                                                                                                                                >  2:261383/1‑151 (MQ=255)             ggAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAAttt                                                                                                                               <  1:232367/151‑1 (MQ=255)              gAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTtc                                                                                                                              >  1:217488/1‑151 (MQ=255)               aaTCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTtct                                                                                                                             <  2:604881/151‑1 (MQ=255)               aaTCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTtct                                                                                                                             <  2:42846/151‑1 (MQ=255)                  cGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCa                                                                                                                          <  1:69148/151‑1 (MQ=255)                   gCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCAt                                                                                                                         <  2:579640/151‑1 (MQ=255)                     ccATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCg                                                                                                                       <  1:560693/151‑1 (MQ=255)                             cACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAAcc                                                                                                               <  1:278968/151‑1 (MQ=255)                                cAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCgg                                                                                                            >  1:331824/1‑151 (MQ=255)                                 aGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGc                                                                                                           >  1:878964/1‑151 (MQ=255)                                      gTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAAt                                                                                                        <  1:32413/149‑1 (MQ=255)                                       tAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATg                                                                                                       >  1:261764/1‑149 (MQ=255)                                         aCGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCg                                                                                                   <  1:385657/151‑1 (MQ=255)                                          cGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGc                                                                                                  <  1:329148/151‑1 (MQ=255)                                            ccgtaccgtaATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCACCGCCATAACCCGGCAATGCTCGc                                                                                                  <  2:658507/149‑1 (MQ=255)                                                accgtaATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCgg                                                                                             <  1:164634/150‑1 (MQ=255)                                                        tAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAAc                                                                                      >  2:562373/1‑149 (MQ=255)                                                          aCCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCg                                                                                  <  2:655880/151‑1 (MQ=255)                                                                    ccGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGcac                                                                        >  1:293249/1‑151 (MQ=255)                                                                    ccGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGca                                                                         <  2:772537/150‑1 (MQ=255)                                                                         aaCGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTg                                                                   >  1:201557/1‑151 (MQ=255)                                                                         aaCGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTg                                                                   >  1:501756/1‑151 (MQ=255)                                                                             ccGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGgcgc                                                               >  1:359537/1‑151 (MQ=255)                                                                               ggacggacGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTa                                                             <  2:72939/151‑1 (MQ=255)                                                                                   ggacgCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCGTCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTc                                                         <  1:716177/151‑1 (MQ=255)                                                                                   ggacgCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTc                                                         >  1:278763/1‑151 (MQ=255)                                                                                   ggacgCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTc                                                         <  2:119311/151‑1 (MQ=255)                                                                                        ccGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACgg                                                    >  2:862061/1‑151 (MQ=255)                                                                                            ttttCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGc                                                >  1:423509/1‑151 (MQ=255)                                                                                             tttCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCg                                               >  2:575075/1‑151 (MQ=255)                                                                                             tttCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCg                                               >  1:476870/1‑151 (MQ=255)                                                                                              ttCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCgg                                              >  2:394978/1‑151 (MQ=255)                                                                                              ttCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCg                                               <  1:899740/150‑1 (MQ=255)                                                                                                      cTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATc                                       <  2:623057/150‑1 (MQ=255)                                                                                                      cTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCg                                      >  2:327115/1‑151 (MQ=255)                                                                                                       ttATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGc                                     >  1:515703/1‑151 (MQ=255)                                                                                                               aTCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGt                             >  2:527645/1‑151 (MQ=255)                                                                                                                 cGGCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTc                           <  2:584111/151‑1 (MQ=255)                                                                                                                   gCAACGGTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCaa                         <  2:878944/151‑1 (MQ=255)                                                                                                                         gTTTCACCCGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAAc                   >  1:796212/1‑151 (MQ=255)                                                                                                                               cccGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTgg             <  2:895614/151‑1 (MQ=255)                                                                                                                                ccGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGc            <  2:608128/151‑1 (MQ=255)                                                                                                                                ccGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGc            <  1:352484/151‑1 (MQ=255)                                                                                                                                 cGGTAACGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGCg           >  1:689838/1‑151 (MQ=255)                                                                                                                                      aCGCTAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGCGGTCa       <  1:667742/150‑1 (MQ=255)                                                                                                                                          tAACCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGCGGTCAGGGc   >  1:808093/1‑150 (MQ=255)                                                                                                                                           aaCCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGCGGTCAGGGCa  <  2:201360/150‑1 (MQ=255)                                                                                                                                           aaCCCGCTCATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGCGGTCAGGGCa  >  1:556737/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                              AATCCATCCGCCGGAATCGCCCATGTCTCACCAGGCTGTAACGCCGTACCGTAATTAACCGTTTGTGCCGGGAACGCCGGACGGACGCCGATTTTCCACTGCTTATGCTGATCGGCAACGGTTTCACCCGGTAACGCTAACCCGCTAATGGTGGCCTGAATTTCTCCATCGCCATAACCCGGCAATGCTCGCACCGGGATAAACAGCGTAGTACGCACACCTGGCGCTAATTCAACGGCTGCGGGTGAATCGCTGACCAGTTCAAGCAAACCACTGGCGGTCAGGGCA  >  NC_000913/2648836‑2649123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑