Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,702,600	T→G	K263Q (AAA→CAA)	era ←	membrane‑associated, 16S rRNA‑binding GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,702,600	0	T	G	100.0%	216.5 / NA	57	K263Q (AAA→CAA)	era	membrane‑associated, 16S rRNA‑binding GTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/29);  total (28/29)

GCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTTACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAACGTTCGAT  >  NC_000913/2702452‑2702735                                                                                                                                                     |                                                                                                                                        gcgcTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGAc                                                                                                                                       >  2:23285/1‑151 (MQ=255)   gcTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGAcgc                                                                                                                                     >  2:641018/1‑151 (MQ=255)   gcTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGAcgc                                                                                                                                     <  2:498195/151‑1 (MQ=255)    cTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGAcgcg                                                                                                                                    >  1:271698/1‑151 (MQ=255)      gCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGAcgcg                                                                                                                                    <  2:192438/149‑1 (MQ=255)      gCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACgcgc                                                                                                                                   <  2:621201/150‑1 (MQ=255)       ccAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCtt                                                                                                                                 <  1:589430/151‑1 (MQ=255)               ccATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCg                                                                                                                         >  2:757318/1‑151 (MQ=255)                 aTCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGAt                                                                                                                       <  1:402036/151‑1 (MQ=255)                  tCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATg                                                                                                                      >  2:650890/1‑151 (MQ=255)                     gAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGtt                                                                                                                   >  1:87049/1‑151 (MQ=255)                       gTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGtttt                                                                                                                 <  2:576764/151‑1 (MQ=255)                          aCTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGa                                                                                                               <  1:854913/150‑1 (MQ=255)                            tctTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCt                                                                                                            >  1:26099/1‑151 (MQ=255)                                 aaGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGcc                                                                                                       >  1:318482/1‑151 (MQ=255)                                    aTCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCt                                                                                                    <  1:881509/151‑1 (MQ=255)                                        tCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTttgt                                                                                                >  1:250904/1‑151 (MQ=255)                                         cAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTttgtt                                                                                               <  2:638822/151‑1 (MQ=255)                                            cGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGcc                                                                                            >  1:558864/1‑151 (MQ=255)                                            cGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGcc                                                                                            >  2:382927/1‑151 (MQ=255)                                                 ccGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGa                                                                                       <  1:134050/151‑1 (MQ=255)                                                    agaCTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCa                                                                                    <  2:878024/151‑1 (MQ=255)                                                      aCTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCAtc                                                                                  <  1:114307/151‑1 (MQ=255)                                                        tGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCAtctt                                                                                >  1:423983/1‑151 (MQ=255)                                                           gcAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCAtcttctt                                                                             <  1:405113/151‑1 (MQ=255)                                                            cAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCAtcttcttc                                                                            <  1:170370/151‑1 (MQ=255)                                                             aGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCAtcttcttct                                                                           >  1:582053/1‑151 (MQ=255)                                                               tGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGc                                                                         >  1:887959/1‑151 (MQ=255)                                                                    gTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTc                                                                    >  1:732219/1‑151 (MQ=255)                                                                     ttCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTc                                                                    >  2:694136/1‑150 (MQ=255)                                                                     ttCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTc                                                                    >  2:85754/1‑150 (MQ=255)                                                                          cgtcgGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCt                                                               <  2:263388/150‑1 (MQ=255)                                                                             cgGCCCAACCGGATTTCACTTTTACCAACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCaa                                                            <  2:773305/150‑1 (MQ=255)                                                                              ggCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACg                                                          <  1:172434/151‑1 (MQ=255)                                                                                 ccAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACg                                                          >  1:100928/1‑148 (MQ=255)                                                                                 ccAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACg                                                          >  2:905784/1‑148 (MQ=255)                                                                                 ccAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACg                                                          >  1:40456/1‑148 (MQ=255)                                                                                   aaCCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAAt                                                     <  2:797835/151‑1 (MQ=255)                                                                                      cGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCaa                                                  <  1:679748/151‑1 (MQ=255)                                                                                      cGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCa                                                   >  2:729745/1‑150 (MQ=255)                                                                                        gATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAAc                                                >  1:896829/1‑151 (MQ=255)                                                                                            tCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGt                                             <  1:624940/150‑1 (MQ=255)                                                                                                 tttACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATg                                        <  2:366028/150‑1 (MQ=255)                                                                                                 tttACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGt                                       >  2:784748/1‑151 (MQ=255)                                                                                                  ttACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAACCCCGATGTTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATTTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGt                                       <  2:866343/150‑1 (MQ=255)                                                                                                    aCCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCa                                     <  1:654314/150‑1 (MQ=255)                                                                                                       cacaGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAac                                 <  2:632722/151‑1 (MQ=255)                                                                                                            ttCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACcg                            >  1:770490/1‑151 (MQ=255)                                                                                                                     aaCAGGCGCTTCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGcgttcgtt                    >  2:797909/1‑150 (MQ=255)                                                                                                                               tCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGaaa          <  1:904032/150‑1 (MQ=255)                                                                                                                               tCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGaa           <  2:835950/149‑1 (MQ=255)                                                                                                                               tCGAACATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAAc         >  1:254639/1‑151 (MQ=255)                                                                                                                                  aaCATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAACGtt      >  1:833999/1‑151 (MQ=255)                                                                                                                                  aaCATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAACGtt      <  2:465337/151‑1 (MQ=255)                                                                                                                                  aaCATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAACGtt      <  1:12074/151‑1 (MQ=255)                                                                                                                                    cATTTCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAACGTTCg    >  2:594638/1‑151 (MQ=255)                                                                                                                                       ttCCTGCATGTCTTGACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAACGTTCGAt  <  2:271693/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                        GCGCTGCCAGCCTTCCATCGGAGTTACTCTTAAAGATCGTCAACGTAACCGAGACTGCGCAGTGCGCGTTCGTCGTCGGCCCAACCGGATTTCACTTTTACCCACAGTTCAAGGTGAACAGGCGCTTCGAACATTTCCTGCATGTCTTTACGCGCTTCAATCCCGATGGTTTTGATCTTGGCCCCTTTGTTGCCAATGACCATCTTCTTCTGCCCTTCACGCTCAACGAGAATCAAACCGTTGATGTCATAACCACCGCGTTCGTTAGAGACGAAACGTTCGAT  >  NC_000913/2702452‑2702735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑