Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,704,662 T→G E216D (GAA→GAC)  lepB ← leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,704,6620TG100.0% 231.8 / NA 62E216D (GAA→GAC) lepBleader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/28);  total (34/28)

GAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCG  >  NC_000913/2704514‑2704804
                                                                                                                                                    |                                                                                                                                              
gaggaACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTc                                                                                                                                              >  1:616062/1‑151 (MQ=255)
  ggaACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGt                                                                                                                                            <  1:893189/151‑1 (MQ=255)
    aaCAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGttt                                                                                                                                          <  2:516393/151‑1 (MQ=255)
       aaTCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTc                                                                                                                                       <  2:854944/151‑1 (MQ=255)
          ccAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGc                                                                                                                                    <  2:367969/151‑1 (MQ=255)
           cAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCa                                                                                                                                   <  1:747911/151‑1 (MQ=255)
            aGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCAc                                                                                                                                  >  1:774248/1‑151 (MQ=255)
            aGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCAc                                                                                                                                  >  2:590629/1‑151 (MQ=255)
                  ccAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCaaa                                                                                                                            <  2:224340/151‑1 (MQ=255)
                     gttgttGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGaa                                                                                                                         <  2:461597/151‑1 (MQ=255)
                        gttgCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATc                                                                                                                       <  2:412321/150‑1 (MQ=255)
                          tgCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCg                                                                                                                     <  2:918746/150‑1 (MQ=255)
                            cccTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTg                                                                                                                  >  2:794029/1‑151 (MQ=255)
                             ccTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTgg                                                                                                                 <  1:43955/151‑1 (MQ=255)
                             ccTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTgg                                                                                                                 >  1:216952/1‑151 (MQ=255)
                              cTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGt                                                                                                                >  1:866055/1‑151 (MQ=255)
                                ggCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTc                                                                                                               <  2:848912/150‑1 (MQ=255)
                                   tgctgGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCtt                                                                                                           >  2:802013/1‑151 (MQ=255)
                                     ctgGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTcc                                                                                                         >  1:87665/1‑151 (MQ=255)
                                           aTACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCccacc                                                                                                    >  1:380291/1‑150 (MQ=255)
                                             aCATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCAtt                                                                                                 >  2:798875/1‑151 (MQ=255)
                                                      ccTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTg                                                                                         >  1:17810/1‑150 (MQ=255)
                                                      ccTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTg                                                                                         <  2:428739/150‑1 (MQ=255)
                                                         gATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTgaga                                                                                      >  2:59384/1‑150 (MQ=255)
                                                              tGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCt                                                                                >  2:511278/1‑151 (MQ=255)
                                                               gcgcAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACTGCGTGAGAAGGTCt                                                                                <  2:15546/150‑1 (MQ=255)
                                                               gcgcAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCt                                                                                <  1:590525/150‑1 (MQ=255)
                                                                cgcAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTg                                                                               >  1:755363/1‑150 (MQ=255)
                                                                 gcAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGa                                                                              >  1:360037/1‑150 (MQ=255)
                                                                  cAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAAc                                                                            <  1:287104/151‑1 (MQ=255)
                                                                              tCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCgg                                                                >  1:3948/1‑151 (MQ=255)
                                                                               cAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGt                                                               <  1:116659/151‑1 (MQ=255)
                                                                                     gCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACg                                                         >  2:176630/1‑151 (MQ=255)
                                                                                       ggTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGtt                                                       <  2:774543/151‑1 (MQ=255)
                                                                                             tCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGt                                                  >  1:523672/1‑150 (MQ=255)
                                                                                             tCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGt                                                  >  1:18911/1‑150 (MQ=255)
                                                                                              cacaTCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAg                                                <  2:685198/151‑1 (MQ=255)
                                                                                               acaTCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAgg                                               >  2:348061/1‑151 (MQ=255)
                                                                                                 aTCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGCGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTg                                             <  2:46711/151‑1 (MQ=255)
                                                                                                       cAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCCCGGTTCCACGTTTGAGTAGGTGACCgg                                        >  2:606295/1‑150 (MQ=255)
                                                                                                               ctTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGcgcg                                 <  2:752529/149‑1 (MQ=255)
                                                                                                                tttACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGttt                              >  2:522518/1‑151 (MQ=255)
                                                                                                                 ttACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGtttt                             >  2:390371/1‑151 (MQ=255)
                                                                                                                 ttACGCTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGtt                               <  1:591879/149‑1 (MQ=255)
                                                                                                                      cTCGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCacac                        >  1:425040/1‑151 (MQ=255)
                                                                                                                        cGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCacac                        <  1:612122/149‑1 (MQ=255)
                                                                                                                        cGGAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACg                       <  2:468060/150‑1 (MQ=255)
                                                                                                                         ggAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGcc                     <  1:590644/151‑1 (MQ=255)
                                                                                                                         ggAAAGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGcc                     >  2:875020/1‑151 (MQ=255)
                                                                                                                             aGACGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGc                 <  1:9449/151‑1 (MQ=255)
                                                                                                                               aCGAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGcc                <  2:665727/150‑1 (MQ=255)
                                                                                                                                 gAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATGGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGcc                >  1:795124/1‑148 (MQ=255)
                                                                                                                                 gAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCgg              >  2:908948/1‑150 (MQ=255)
                                                                                                                                 gAATTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGa             <  2:786961/151‑1 (MQ=255)
                                                                                                                                   aTTCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGaa            >  2:780588/1‑150 (MQ=255)
                                                                                                                                    ttCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACt          >  1:222997/1‑151 (MQ=255)
                                                                                                                                    ttCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACt          >  2:369244/1‑151 (MQ=255)
                                                                                                                                     tCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTg         >  1:188672/1‑151 (MQ=255)
                                                                                                                                     tCCATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTg         >  1:142758/1‑151 (MQ=255)
                                                                                                                                       cATTTTCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCa       <  1:526066/151‑1 (MQ=255)
                                                                                                                                          tttCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATcc    >  1:630789/1‑151 (MQ=255)
                                                                                                                                            tCTTTGGTGTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCg  >  1:35833/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCCCCACCTGATCCTGCGCAATCGGCACTGTCAGAATGCGGTGCGTCACATCACCCAGTGTCTCTTTACGCTCGGAAAGACGAATTCCATTTTCTTTGGTTTCGTTTTTCGGCACTTCAAAGAATCCGCTGGTCGCTTCCCCACCATTACGGCGTGAGAAGGTCTGAACGAAATCGCTCGGTTCCACGTTTGAGTAGGTGACCGGCAGCGCGTTTTCACACGCCTGGCCGGAACTGCATCCCG  >  NC_000913/2704514‑2704804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑