| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,724,576 | A→C | Y391D (TAC→GAC) | kgtP ← | alpha‑ketoglutarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,724,576 | 0 | A | C | 98.2% | 202.0 / ‑4.6 | 56 | Y391D (TAC→GAC) | kgtP | alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (26/29); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACC > NC_000913/2724430‑2724721 | cTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTc < 2:512022/150‑1 (MQ=255) ggCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGtcctcc < 2:47170/149‑1 (MQ=255) aaCTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTccgccg > 2:712048/1‑150 (MQ=255) cTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAac > 1:730161/1‑151 (MQ=255) cGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCaa < 1:163201/151‑1 (MQ=255) gTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCaaa < 2:431015/151‑1 (MQ=255) cTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAatat > 2:309548/1‑150 (MQ=255) cTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAatat > 1:485364/1‑150 (MQ=255) aaaGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAAtata < 2:149703/149‑1 (MQ=255) aaaGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGc > 1:918384/1‑151 (MQ=255) gACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCAtt < 2:182868/151‑1 (MQ=255) aCGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCAtt > 1:700776/1‑150 (MQ=255) ccccTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGAcc < 2:776503/151‑1 (MQ=255) ccccTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGAcc > 1:562050/1‑151 (MQ=255) cccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCatat < 1:371706/151‑1 (MQ=255) cccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCata < 2:899339/150‑1 (MQ=255) ccttTGCGATGTATCAGCAAAGAAACCAAAAAGTCTCTCACGGCCATAAAAGTCCCATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACCCCGCTACGTCCTACGCCGAACCTCAAAATATAGCATTAGc < 2:587300/140‑1 (MQ=255) ccttTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATg < 2:672380/151‑1 (MQ=255) ttGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGAc < 2:643612/150‑1 (MQ=255) tGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGAc > 2:156323/1‑149 (MQ=255) gATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGAcc > 1:546305/1‑151 (MQ=255) gTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCaa < 2:549359/150‑1 (MQ=255) gTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCaa < 1:184426/150‑1 (MQ=255) cAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAAt > 1:855151/1‑151 (MQ=255) aGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATgcg > 1:738244/1‑151 (MQ=255) aGAAACCAGAAACGCCACCACGGCCATCAAGGGCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGAGAACGCATATGACAGACCAACGACTAATgcg > 2:538365/1‑151 (MQ=255) aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGcgcg > 1:568440/1‑151 (MQ=255) aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGcgcg > 1:755706/1‑151 (MQ=255) aaaCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGcgcg > 2:148044/1‑151 (MQ=255) gAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCtg < 2:600953/151‑1 (MQ=255) aaCGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCtgtg < 2:670957/151‑1 (MQ=255) ccaccaCGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCA‑TAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCgg < 2:184477/150‑1 (MQ=255) gcatacaGGTCAGATCCCAGGAGAAGGCTGTTTCCATTCCTATTGATTTCTTCGAGATCCCTACGTACTCCGCCGATTTACCAGGTATAGTTTTAGCGACAGCCTATGACAGACCATCGTCTCGTGCGCGACCGTCTGCCGGGAACAtct > 2:113638/7‑150 (MQ=255) aTCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACAtctc > 1:151492/1‑149 (MQ=255) aGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGTGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCtt < 1:479860/151‑1 (MQ=255) aGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCtt > 1:325259/1‑151 (MQ=255) ggTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTc < 1:866939/151‑1 (MQ=255) cacaTACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAg < 2:812569/150‑1 (MQ=255) gaagaaGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtg > 1:834389/1‑151 (MQ=255) agaagGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACtgat < 2:332848/151‑1 (MQ=255) ttCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGtata < 2:187995/150‑1 (MQ=255) tCCATTCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATaa < 2:676812/150‑1 (MQ=255) ttCCTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACt > 1:276166/1‑151 (MQ=255) ccTATTGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTc > 2:852828/1‑150 (MQ=255) tGATTTCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTAt < 2:502167/150‑1 (MQ=255) tttCAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagc > 2:152458/1‑151 (MQ=255) cAGCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATcagcag < 1:112828/150‑1 (MQ=255) aGCGACAACGCCACGTCCTCCGCCGAACCACCAAATATAGCATCACCGACCGCATACGACAGACCAACCCCTAATCCGCGAACCTGTGCCGGGAACATCCCACCCTTCAGCACCCCACTGCTTGACGTATAAAAACTCACTATcagc > 2:184447/1‑147 (MQ=255) gCGACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGc < 2:532640/151‑1 (MQ=255) gACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac > 1:739806/1‑151 (MQ=255) gACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac > 1:85032/1‑151 (MQ=255) gACAACGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcac > 2:310494/1‑151 (MQ=255) aaCGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGcacaca < 2:169372/151‑1 (MQ=255) aCGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACAt < 1:325807/151‑1 (MQ=255) cGCTACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATc < 2:596745/151‑1 (MQ=255) tACGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCATACATCAcc > 1:265563/1‑151 (MQ=255) aCGTCCTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCAcc < 1:861168/150‑1 (MQ=255) | CTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGCCGAACCACCAAATATAGCATTAGCGACCGCATATGACAGACCAACGCCTAATGCGCGAACCTGTGCCGGGAACATCTCAGCCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACC > NC_000913/2724430‑2724721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |