Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,753,923	A→C	K107Q (AAA→CAA)	bamE →	lipoprotein component of BamABCDE OM biogenesis complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,753,923	0	A	C	100.0%	221.4 / NA	56	K107Q (AAA→CAA)	bamE	lipoprotein component of BamABCDE OM biogenesis complex
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/33);  total (23/33)

CGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCG  >  NC_000913/2753775‑2754059                                                                                                                                                     |                                                                                                                                         cGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACcaa                                                                                                                                        <  1:542365/151‑1 (MQ=255)      tGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTg                                                                                                                                    <  2:708812/150‑1 (MQ=255)       gggTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTgcg                                                                                                                                  <  2:201491/151‑1 (MQ=255)       gggTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTgcg                                                                                                                                  <  1:580660/151‑1 (MQ=255)       gggTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTgc                                                                                                                                   <  2:922552/150‑1 (MQ=255)           acacCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGa                                                                                                                              <  2:339721/151‑1 (MQ=255)            cacCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTg                                                                                                                               >  1:83550/1‑149 (MQ=255)            cacCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGa                                                                                                                              <  2:341848/150‑1 (MQ=255)              ccGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTg                                                                                                                           >  1:82758/1‑151 (MQ=255)                gCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTgg                                                                                                                          >  2:8208/1‑150 (MQ=255)                gCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGt                                                                                                                         >  2:296553/1‑151 (MQ=255)                 cTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGt                                                                                                                         >  2:204397/1‑150 (MQ=255)                  tGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTa                                                                                                                        >  1:333070/1‑150 (MQ=255)                   gATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTa                                                                                                                        <  2:283352/149‑1 (MQ=255)                   gATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAAc                                                                                                                      >  2:676730/1‑151 (MQ=255)                    aTGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACt                                                                                                                     >  2:795273/1‑151 (MQ=255)                            tCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACtaataata                                                                                                              >  2:315112/1‑150 (MQ=255)                             ccATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATaaa                                                                                                            <  2:230742/151‑1 (MQ=255)                             ccATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATaaa                                                                                                            <  1:324280/151‑1 (MQ=255)                                    gTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGGCCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTg                                                                                                        <  1:695676/148‑1 (MQ=255)                                      aCGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCtctc                                                                                                   >  1:619110/1‑151 (MQ=255)                                      aCGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCtctc                                                                                                   <  1:626010/151‑1 (MQ=255)                                         aaTACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCaaa                                                                                                <  2:838029/151‑1 (MQ=255)                                         aaTACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCaaa                                                                                                <  2:6756/151‑1 (MQ=255)                                          aTACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAg                                                                                               <  1:552755/151‑1 (MQ=255)                                          aTACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAg                                                                                               <  2:91194/151‑1 (MQ=255)                                                ggTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGtt                                                                                          <  1:467581/150‑1 (MQ=255)                                                ggTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGtt                                                                                          <  1:852190/150‑1 (MQ=255)                                                ggTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTa                                                                                         <  1:18443/151‑1 (MQ=255)                                                 gTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTaa                                                                                        <  2:44382/151‑1 (MQ=255)                                                 gTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTa                                                                                         >  2:910750/1‑150 (MQ=255)                                                      aTGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTaaaaaa                                                                                    <  1:727263/150‑1 (MQ=255)                                                               gCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAAt                                                                          >  1:547741/1‑151 (MQ=255)                                                                    cAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGc                                                                      >  1:562375/1‑150 (MQ=255)                                                                          ggTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCtttt                                                               <  2:275473/151‑1 (MQ=255)                                                                          ggTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCtttt                                                               >  1:206318/1‑151 (MQ=255)                                                                             cATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtc                                                            <  2:26841/151‑1 (MQ=255)                                                                             cATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtc                                                            <  1:138869/151‑1 (MQ=255)                                                                                gAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgt                                                         >  2:838839/1‑151 (MQ=255)                                                                                 aaGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtc                                                        <  2:97428/151‑1 (MQ=255)                                                                                         aCTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttattt                                                <  2:416212/151‑1 (MQ=255)                                                                                                       gacgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAttttt                                  >  1:586927/1‑151 (MQ=255)                                                                                                       gacgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAtttt                                   <  2:397943/150‑1 (MQ=255)                                                                                                       gacgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAttt                                    <  1:676746/149‑1 (MQ=255)                                                                                                        acgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAttttt                                  >  1:614940/1‑150 (MQ=255)                                                                                                           ctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGc                              >  2:593138/1‑151 (MQ=255)                                                                                                                  ttAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGtt                         <  1:569037/149‑1 (MQ=255)                                                                                                                   tAACAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGcc                      >  2:168080/1‑151 (MQ=255)                                                                                                                      cAGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGc                   <  1:437268/151‑1 (MQ=255)                                                                                                                       aGTAGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCg                  <  2:335767/151‑1 (MQ=255)                                                                                                                          aGCGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGaa                <  2:70231/150‑1 (MQ=255)                                                                                                                            cGGTGTGTTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGct             >  1:35108/1‑151 (MQ=255)                                                                                                                                  gtTGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCg       >  2:685158/1‑151 (MQ=255)                                                                                                                                   ttGACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCgg      >  2:208135/1‑151 (MQ=255)                                                                                                                                     gACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt    <  1:487096/151‑1 (MQ=255)                                                                                                                                     gACCAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt    <  1:795291/151‑1 (MQ=255)                                                                                                                                       ccAATATTGATAACCAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCg  >  2:444513/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                         CGCATTGGGTACACCGCTGATGTCCGATCCATTTGGTACGAATACCTGGTTCTATGTCTTCCGCCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCG  >  NC_000913/2753775‑2754059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑