Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,674	T→G	L287* (TTA→TGA)	proV →	glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,674	0	T	G	100.0%	216.9 / NA	57	L287* (TTA→TGA)	proV	glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/26);  total (31/26)

TGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGC  >  NC_000913/2805527‑2805817                                                                                                                                                    |                                                                                                                                                tGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAAt                                                                                                                                              >  1:471584/1‑151 (MQ=255)   cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTc                                                                                                                                            <  2:679437/151‑1 (MQ=255)   cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTc                                                                                                                                            >  1:366248/1‑151 (MQ=255)   cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTc                                                                                                                                            >  1:381952/1‑151 (MQ=255)      aaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGt                                                                                                                                          >  1:792290/1‑150 (MQ=255)      aaTGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGt                                                                                                                                          >  2:527439/1‑150 (MQ=255)        tGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTaa                                                                                                                                        >  2:639520/1‑150 (MQ=255)            gAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAAccc                                                                                                                                   <  1:573983/151‑1 (MQ=255)            gAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAAccc                                                                                                                                   <  1:548885/151‑1 (MQ=255)             aaGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAAcccc                                                                                                                                  <  1:267903/151‑1 (MQ=255)              aGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCt                                                                                                                                 <  2:537227/151‑1 (MQ=255)                 ggTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGc                                                                                                                              >  1:657723/1‑151 (MQ=255)                          cGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCa                                                                                                                     <  2:5324/151‑1 (MQ=255)                            gCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACg                                                                                                                   <  2:884221/151‑1 (MQ=255)                                      tGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTg                                                                                                         >  1:808782/1‑151 (MQ=255)                                       gAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTg                                                                                                         <  1:78779/150‑1 (MQ=255)                                        aaaTTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGaa                                                                                                       <  1:50475/151‑1 (MQ=255)                                        aaaTTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGa                                                                                                        >  1:27510/1‑150 (MQ=255)                                            tctcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAatt                                                                                                    >  2:504711/1‑150 (MQ=255)                                               cAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAattatt                                                                                                 <  1:461213/150‑1 (MQ=255)                                               cAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAattat                                                                                                  <  1:877462/149‑1 (MQ=255)                                                aataatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGc                                                                                               <  1:262260/151‑1 (MQ=255)                                                 ataatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTg                                                                                                >  2:844667/1‑149 (MQ=255)                                                  taatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGc                                                                                               >  2:660440/1‑149 (MQ=255)                                                   aatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAg                                                                                             <  2:394995/150‑1 (MQ=255)                                                       cGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGa                                                                                        <  1:229959/151‑1 (MQ=255)                                                         gCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGa                                                                                        >  1:344035/1‑149 (MQ=255)                                                          cGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGa                                                                                     <  1:20923/151‑1 (MQ=255)                                                                aTTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATcgcg                                                                                >  2:332943/1‑150 (MQ=255)                                                                aTTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGa                                                                               <  2:875532/151‑1 (MQ=255)                                                                aTTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGa                                                                               <  2:277388/151‑1 (MQ=255)                                                                   aTGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAat                                                                             <  1:492789/150‑1 (MQ=255)                                                                        cGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCt                                                                       <  2:891580/151‑1 (MQ=255)                                                                           aCCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACg                                                                    <  1:290911/151‑1 (MQ=255)                                                                           aCCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACg                                                                    >  2:52403/1‑151 (MQ=255)                                                                                  tcCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCg                                                              >  1:54990/1‑150 (MQ=255)                                                                                   ccGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGa                                                             >  2:593196/1‑150 (MQ=255)                                                                                    cGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                           >  1:494370/1‑151 (MQ=255)                                                                                        gCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCgg                                                       <  1:284674/151‑1 (MQ=255)                                                                                              atatTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGtaata                                                  >  1:631740/1‑150 (MQ=255)                                                                                                   aGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTg                                            >  2:669802/1‑151 (MQ=255)                                                                                                    gTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAATCCCCTGTCTTCGGCCCACGTTCTGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCTGGAATAAGTTTg                                            <  2:698796/150‑1 (MQ=255)                                                                                                       aGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCgg                                        <  1:275498/151‑1 (MQ=255)                                                                                                        ggTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGgc                                       <  2:377032/151‑1 (MQ=255)                                                                                                              cAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGtc                                 >  1:809363/1‑151 (MQ=255)                                                                                                                  gCGAAAGATGTTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCa                             <  1:608195/151‑1 (MQ=255)                                                                                                                  gCGAAAGATATTGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTcc                              >  1:733630/1‑150 (MQ=255)                                                                                                                            ttGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCt                   >  1:203462/1‑151 (MQ=255)                                                                                                                             tGCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACCGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCtt                  >  2:298715/1‑151 (MQ=255)                                                                                                                              gCCCGCCGGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCtt                  >  1:297542/1‑150 (MQ=255)                                                                                                                                ccgccgGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTaa                >  1:919329/1‑150 (MQ=255)                                                                                                                                  gccgGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAAc             <  1:588368/151‑1 (MQ=255)                                                                                                                                    cgGACACCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACcg           >  1:242393/1‑151 (MQ=255)                                                                                                                                         acCGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGtt       >  1:493748/1‑150 (MQ=255)                                                                                                                                           cGAATGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAAc    >  2:203305/1‑151 (MQ=255)                                                                                                                                              aTGGCTGAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACgc  >  1:584781/1‑150 (MQ=255)                                                                                                                                               tGGCTGGATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACgc  >  1:473980/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                TGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGC  >  NC_000913/2805527‑2805817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑