Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,863,239	T→G	L302R (CTG→CGG)	ygbK →	FliA‑regulated DUF1537 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,863,239	0	T	G	100.0%	163.6 / NA	46	L302R (CTG→CGG)	ygbK	FliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/24);  total (22/24)

AGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCG  >  NC_000913/2863093‑2863372                                                                                                                                                   |                                                                                                                                      aGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGc                                                                                                                                   <  1:336055/151‑1 (MQ=255)     ctctCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAgg                                                                                                                               >  2:499763/1‑151 (MQ=255)       ctcCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAgg                                                                                                                               <  1:920067/149‑1 (MQ=255)        tctggTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGaa                                                                                                                             >  1:209404/4‑150 (MQ=255)         ccGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGaa                                                                                                                             <  2:548429/149‑1 (MQ=255)         ccGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGaa                                                                                                                             <  2:112133/149‑1 (MQ=255)           ggTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAgtg                                                                                                                         <  2:828786/151‑1 (MQ=255)                       cAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTcc                                                                                                               <  2:331945/149‑1 (MQ=255)                       cAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTc                                                                                                                <  2:715739/148‑1 (MQ=255)                       cAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCAc                                                                                                             >  2:128081/1‑151 (MQ=255)                        aaaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACt                                                                                                            >  2:90800/1‑151 (MQ=255)                         aaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTg                                                                                                           >  2:82088/1‑151 (MQ=255)                         aaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTg                                                                                                           <  1:737743/151‑1 (MQ=255)                         aaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTg                                                                                                           <  2:455426/151‑1 (MQ=255)                              ccAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGtttt                                                                                                      >  1:438531/1‑151 (MQ=255)                               cAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGttttt                                                                                                     <  2:662344/151‑1 (MQ=255)                               cAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGttttt                                                                                                     <  1:785019/151‑1 (MQ=255)                               cAACCGACAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGttttt                                                                                                     >  2:596879/1‑151 (MQ=255)                                aaCCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTg                                                                                                    >  1:110153/1‑151 (MQ=255)                                 aCCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGc                                                                                                   >  2:889218/1‑151 (MQ=255)                                         gTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGcc                                                                                            >  2:378610/1‑150 (MQ=255)                                         gTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCa                                                                                           <  2:385397/151‑1 (MQ=255)                                          tAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGcc                                                                                            >  2:171829/1‑149 (MQ=255)                                           aGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGc                                                                                         >  2:260333/1‑151 (MQ=255)                                              acaTTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACt                                                                                      >  1:709913/1‑151 (MQ=255)                                                  tACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACg                                                                                  >  2:611453/1‑151 (MQ=255)                                                    ccGTCAAATTGCACCGGCCCGTGATGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCGGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAGGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACg                                                                                  <  2:759111/149‑1 (MQ=255)                                                                 ccAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAAtt                                                                    <  1:742490/150‑1 (MQ=255)                                                                      ccGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTTCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCaa                                                                 >  2:54822/1‑148 (MQ=255)                                                                            aGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATAc                                                        >  1:799932/1‑151 (MQ=255)                                                                             gTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACg                                                       >  2:170110/1‑151 (MQ=255)                                                                              ttGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATAc                                                        <  1:868479/149‑1 (MQ=255)                                                                                 aTGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGc                                                   >  1:599881/1‑151 (MQ=255)                                                                                  tgtgGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGc                                                   <  1:710920/150‑1 (MQ=255)                                                                                       cACGCTGCCCCTCAATTGAACCTCTGGCCGCTTATGCACACGATCTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCACCGCTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACaaaa                                             <  2:658701/151‑1 (MQ=255)                                                                                          gCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGc                                          >  2:712279/1‑151 (MQ=255)                                                                                              ccTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCa                                        <  1:467257/149‑1 (MQ=255)                                                                                                    aaTTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGc                                 <  2:891555/150‑1 (MQ=255)                                                                                                         aaaCTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGt                              >  2:10934/1‑148 (MQ=255)                                                                                                         aaaCTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGACAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGt                              >  1:535527/1‑148 (MQ=255)                                                                                                          aaCTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGa                           >  1:225174/1‑150 (MQ=255)                                                                                                                  ccGCTTATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGtt                  <  2:228967/151‑1 (MQ=255)                                                                                                                       tATGCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTtct              <  1:123394/150‑1 (MQ=255)                                                                                                                          gCACACGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAAc          <  1:890614/151‑1 (MQ=255)                                                                                                                             cacGAACTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTa        <  2:871952/150‑1 (MQ=255)                                                                                                                                   cTGGCAGAGTGGGTTCGGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAgcggcg  <  2:207830/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                      AGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGTTTTTGCCACCGCCAGCACTGACGCATTGGCAGCAATTCAACAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCG  >  NC_000913/2863093‑2863372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑