Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,027,187	A→C	K23Q (AAA→CAA)	ghxQ →	guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,027,187	0	A	C	100.0%	213.9 / NA	56	K23Q (AAA→CAA)	ghxQ	guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/25);  total (31/25)

CTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTG  >  NC_000913/3027040‑3027332                                                                                                                                                    |                                                                                                                                                  cTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTcaa                                                                                                                                                 >  1:165875/1‑150 (MQ=255)      gCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTAcc                                                                                                                                           >  1:115391/1‑151 (MQ=255)       cACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCg                                                                                                                                          <  1:235351/151‑1 (MQ=255)         ccTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCt                                                                                                                                        <  2:576817/151‑1 (MQ=255)         ccTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCt                                                                                                                                        <  1:374713/151‑1 (MQ=255)            aCGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCACGt                                                                                                                                     >  1:709792/1‑151 (MQ=255)              gTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTgg                                                                                                                                   >  2:817873/1‑151 (MQ=255)                    ggTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTa                                                                                                                              <  1:917984/150‑1 (MQ=255)                    ggTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTAc                                                                                                                             >  1:337998/1‑151 (MQ=255)                        gtcTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGtt                                                                                                                         >  2:902361/1‑151 (MQ=255)                        gtcTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGtt                                                                                                                         <  2:664713/151‑1 (MQ=255)                             gtgtACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCa                                                                                                                    <  2:231273/151‑1 (MQ=255)                              tgtACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTc                                                                                                                     >  2:246878/1‑149 (MQ=255)                                 aCGAACGCAACTAATAATAAAACTTTAACATTCTCGTGAGGACATCATTATGTCTGGCGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTTGCAGTACCGTTCGTCAg                                                                                                                   >  2:384511/1‑148 (MQ=255)                                   gAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTGCGTCAGGAAGt                                                                                                              <  2:837693/151‑1 (MQ=255)                                     aCGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTAc                                                                                                            >  2:831445/1‑151 (MQ=255)                                             aataataaAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTActggctg                                                                                                      <  1:436986/149‑1 (MQ=255)                                                aataaAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCtt                                                                                                  >  2:368646/1‑150 (MQ=255)                                                 ataaAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCtt                                                                                                  >  2:65253/1‑149 (MQ=255)                                                 ataaAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTa                                                                                                 >  1:504010/1‑150 (MQ=255)                                                    aaaCTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCGGTACCGTTCGTCAGGAAGTACTGGCTGGCTTaa                                                                                                >  1:271420/1‑148 (MQ=255)                                                    aaaCTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGa                                                                                             <  2:451259/151‑1 (MQ=255)                                                         ttAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCtt                                                                                         <  1:879880/150‑1 (MQ=255)                                                             cATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTgg                                                                                    <  1:652448/151‑1 (MQ=255)                                                                 cTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCAt                                                                                >  1:408854/1‑151 (MQ=255)                                                                    gTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGt                                                                             >  1:736304/1‑151 (MQ=255)                                                                    gTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGt                                                                             <  2:453685/151‑1 (MQ=255)                                                                       aGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTa                                                                          <  1:157657/151‑1 (MQ=255)                                                                          aCATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTc                                                                       <  1:497607/151‑1 (MQ=255)                                                                              catTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGtt                                                                   >  1:852727/1‑151 (MQ=255)                                                                                  aTGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcg                                                               <  2:150725/151‑1 (MQ=255)                                                                                  aTGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcg                                                               <  1:809608/151‑1 (MQ=255)                                                                                    gTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcgtc                                                             <  1:698924/151‑1 (MQ=255)                                                                                    gTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcgt                                                              <  2:266782/150‑1 (MQ=255)                                                                                          agaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCg                                                       >  1:50364/1‑151 (MQ=255)                                                                                                       acacCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCa                                          <  2:163361/151‑1 (MQ=255)                                                                                                        cacCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCa                                          >  2:208111/1‑150 (MQ=255)                                                                                                         acCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCaa                                         >  1:828244/1‑150 (MQ=255)                                                                                                            ggACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAg                                       >  2:70714/1‑149 (MQ=255)                                                                                                            ggACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCCCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAg                                       >  1:389177/1‑149 (MQ=255)                                                                                                              aCGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAgg                                   >  1:91077/1‑151 (MQ=255)                                                                                                              aCGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAgg                                   <  1:887313/151‑1 (MQ=255)                                                                                                                   ccAAAGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTtc                              <  2:855706/151‑1 (MQ=255)                                                                                                                     aaaGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTtcct                            <  1:902381/151‑1 (MQ=255)                                                                                                                      aaGCCACCGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGAACTTTCTGGCCATGGTTTATTCAGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGtttttc                             >  2:280664/1‑147 (MQ=255)                                                                                                                       aGCCACAGGGCGCGCTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTtcctcc                          >  2:709890/1‑151 (MQ=255)                                                                                                                                gcgcgcTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCtgt                 >  1:341121/1‑151 (MQ=255)                                                                                                                                 cgcgcTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCtgtg                >  2:685206/1‑151 (MQ=255)                                                                                                                                     cTGGATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgtttg            >  1:787372/1‑151 (MQ=255)                                                                                                                                       ggATAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgtttg            <  2:709774/149‑1 (MQ=255)                                                                                                                                         aTAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGc        >  1:154611/1‑151 (MQ=255)                                                                                                                                          tAATTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGcc       >  1:905167/1‑151 (MQ=255)                                                                                                                                           aaTTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGcc       >  2:363162/1‑150 (MQ=255)                                                                                                                                           aaTTATTTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGcc       >  2:238656/1‑150 (MQ=255)                                                                                                                                            attattTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCAc     <  1:375302/151‑1 (MQ=255)                                                                                                                                             ttattTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCAcc    >  2:681282/1‑151 (MQ=255)                                                                                                                                               attTTCAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCTCCTg  <  2:334503/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTG  >  NC_000913/3027040‑3027332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑