Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,062,456	A→C	E536A (GAA→GCA)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,062,456	0	A	C	100.0%	155.7 / NA	44	E536A (GAA→GCA)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/23);  total (21/23)

CCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCC  >  NC_000913/3062333‑3062603                                                                                                                            |                                                                                                                                                    ccgccgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTAt                                                                                                                                  <  1:247783/143‑1 (MQ=255) ccgccgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGcc                                                                                                                          >  2:158088/1‑151 (MQ=255)   gccgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCa                                                                                                                         >  1:245644/1‑150 (MQ=255)     cgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGc                                                                                                                      <  1:360475/151‑1 (MQ=255)          ccgccgCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAgt                                                                                                                  <  2:227477/150‑1 (MQ=255)                      aCGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGt                                                                                                     >  1:757375/1‑151 (MQ=255)                          ccTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGAtt                                                                                                 >  2:606358/1‑151 (MQ=255)                          ccTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGAtt                                                                                                 <  1:861826/151‑1 (MQ=255)                                  aCGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCaa                                                                                           >  2:410173/1‑149 (MQ=255)                                    gccgcACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCt                                                                                       >  2:518888/1‑151 (MQ=255)                                     ccgcACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTa                                                                                      <  1:390447/151‑1 (MQ=255)                                           aGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTAtcatca                                                                                <  2:906039/151‑1 (MQ=255)                                             cATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAg                                                                               >  1:701119/1‑150 (MQ=255)                                                aaCGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCt                                                                            <  1:594246/150‑1 (MQ=255)                                                 aCGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTga                                                                          <  1:31924/151‑1 (MQ=255)                                                    aaaaCCTGCTGGCTGCCGCTCTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGGAAAGCTATCATCAGTCTgaga                                                                        <  2:728723/150‑1 (MQ=255)                                                     aaaCCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGaaa                                                                      <  1:214107/151‑1 (MQ=255)                                                      aaCCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTgaga                                                                        >  2:61451/1‑148 (MQ=255)                                                        ccTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCg                                                                   <  2:187653/151‑1 (MQ=255)                                                        ccTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCg                                                                   <  2:405108/151‑1 (MQ=255)                                                              ggctgCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCt                                                               <  1:358616/149‑1 (MQ=255)                                                                ctgCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCg                                                            >  2:537555/1‑150 (MQ=255)                                                                 tgCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCg                                                            >  2:454002/1‑149 (MQ=255)                                                                  gCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGa                                                           <  1:662807/149‑1 (MQ=255)                                                                  gCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGt                                                         <  2:742283/151‑1 (MQ=255)                                                                   ccGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGtt                                                        >  1:837500/1‑151 (MQ=255)                                                                   ccGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGtt                                                        <  1:611812/151‑1 (MQ=255)                                                                             tGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCAtt                                              >  2:702742/1‑151 (MQ=255)                                                                                cGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCAttgtt                                           >  1:677505/1‑151 (MQ=255)                                                                                cGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCAttgtt                                           <  2:688276/151‑1 (MQ=255)                                                                                    cgcgTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTgc                                         <  1:821473/149‑1 (MQ=255)                                                                                          cgcgCCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACg                                  <  1:248963/150‑1 (MQ=255)                                                                                           gcgcCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACgg                                 >  1:570329/1‑150 (MQ=255)                                                                                           gcgcCACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACgg                                 >  1:544660/1‑150 (MQ=255)                                                                                               cACCCTGGGTGAAATTTCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGc                              >  1:865247/1‑149 (MQ=255)                                                                                                             tttCCGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGAc                >  2:663382/1‑149 (MQ=255)                                                                                                                ccGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATgg           <  2:120877/151‑1 (MQ=255)                                                                                                                 cGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATgg           >  2:272029/1‑150 (MQ=255)                                                                                                                 cGATGCGCTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGGGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGgt          <  1:250206/151‑1 (MQ=255)                                                                                                                     gcgcTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGgtcgt       <  2:552801/150‑1 (MQ=255)                                                                                                                      cgcTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGgtcgtc      >  1:562998/1‑150 (MQ=255)                                                                                                                      cgcTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGtcgtcg     <  2:291924/151‑1 (MQ=255)                                                                                                                       gcTGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGGAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCGGTTCCTTGCCGACAATGGTGGTTGc    >  1:382795/1‑151 (MQ=255)                                                                                                                         tGGCAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGccc  >  2:854463/1‑151 (MQ=255)                                                                                                                            |                                                                                                                                                    CCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCC  >  NC_000913/3062333‑3062603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑