Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,833	T→G	E360A (GAA→GCA)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,833	0	T	G	100.0%	190.8 / NA	52	E360A (GAA→GCA)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/33);  total (19/33)

CATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCC  >  NC_000913/3084688‑3084973                                                                                                                                                  |                                                                                                                                             cATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGt                                                                                                                                          <  1:896636/150‑1 (MQ=255) cATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTg                                                                                                                                         <  1:197544/151‑1 (MQ=255)       cTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTAcc                                                                                                                                   >  1:206512/1‑151 (MQ=255)         gCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTAcc                                                                                                                                   <  1:290523/149‑1 (MQ=255)            aGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCgg                                                                                                                              <  1:437935/151‑1 (MQ=255)            aGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCgg                                                                                                                              >  2:195302/1‑151 (MQ=255)            aGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCgg                                                                                                                              <  1:695997/151‑1 (MQ=255)             ggTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCgg                                                                                                                              <  1:19250/150‑1 (MQ=255)               tttCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATg                                                                                                                           >  1:105472/1‑151 (MQ=255)                ttCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATg                                                                                                                           <  2:320665/150‑1 (MQ=255)                    cacaTGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCa                                                                                                                      <  1:75428/151‑1 (MQ=255)                     acaTGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAg                                                                                                                     <  1:310568/151‑1 (MQ=255)                         gCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCg                                                                                                                 <  2:166156/151‑1 (MQ=255)                            ttGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGttt                                                                                                              >  1:815516/1‑151 (MQ=255)                            ttGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGt                                                                                                                <  1:356915/149‑1 (MQ=255)                             tGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGttt                                                                                                              <  1:626763/150‑1 (MQ=255)                              gCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGttt                                                                                                              <  2:910967/149‑1 (MQ=255)                               cAGCGCGCGGGGCGCATCTTCTGCAGGCGCGGTCGGCCCCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTttct                                                                                                           <  1:702061/151‑1 (MQ=255)                               cAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTttct                                                                                                           <  2:632339/151‑1 (MQ=255)                                  cgcgcgcgGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTttcttc                                                                                                         >  1:123200/1‑150 (MQ=255)                                   gcgcgcgGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTcac                                                                                                       >  2:511159/1‑151 (MQ=255)                                       gcggcgCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACg                                                                                                    <  2:788580/150‑1 (MQ=255)                                          gcgcATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATc                                                                                                <  1:221488/151‑1 (MQ=255)                                          gcgcATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGCGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATc                                                                                                <  1:119216/151‑1 (MQ=255)                                            gcATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGc                                                                                              <  1:415091/151‑1 (MQ=255)                                                     gCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg                                                                                        <  2:352650/148‑1 (MQ=255)                                                     gCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGccc                                                                                     >  2:864961/1‑151 (MQ=255)                                                      cAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGccc                                                                                     >  1:567117/1‑150 (MQ=255)                                                              gTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAAtgt                                                                            <  1:501373/151‑1 (MQ=255)                                                                     ccGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGc                                                                     >  1:569177/1‑151 (MQ=255)                                                                         gtATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTAt                                                                 >  1:711834/1‑151 (MQ=255)                                                                              cGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCAtt                                                            <  1:71405/151‑1 (MQ=255)                                                                              cGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCAt                                                             <  1:275750/150‑1 (MQ=255)                                                                                   cGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGc                                                       >  2:38168/1‑151 (MQ=255)                                                                                    gTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGcc                                                      <  1:344736/151‑1 (MQ=255)                                                                                       ccACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGt                                                    <  2:835699/150‑1 (MQ=255)                                                                                       ccACGCCGATGATATTAGACACCAGCACGCTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGCTAATGTTGTTGGCGTATTCATTGAGGCCGTa                                                   <  2:438261/151‑1 (MQ=255)                                                                                        cACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGt                                                    >  1:261861/1‑149 (MQ=255)                                                                                        cACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAg                                                  <  2:651939/151‑1 (MQ=255)                                                                                            ccGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCa                                              <  2:599510/151‑1 (MQ=255)                                                                                                tgatATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCg                                           >  2:142789/1‑150 (MQ=255)                                                                                                    atTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACa                                       <  1:265804/150‑1 (MQ=255)                                                                                                        gACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCgg                                  >  2:740804/1‑151 (MQ=255)                                                                                                                cACGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGaa                          <  1:311449/151‑1 (MQ=255)                                                                                                                  cGGTGTGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg                        <  1:450438/151‑1 (MQ=255)                                                                                                                    gtgtGATGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg                        >  1:357366/1‑149 (MQ=255)                                                                                                                          tGCGCAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCtt                >  1:538205/1‑151 (MQ=255)                                                                                                                              cAGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCAt             >  1:403783/1‑150 (MQ=255)                                                                                                                               aGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa           <  2:450018/151‑1 (MQ=255)                                                                                                                               aGTCACCGCACGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa            >  2:907760/1‑150 (MQ=255)                                                                                                                                        aCGACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGccc  <  2:847661/151‑1 (MQ=255)                                                                                                                                          gACCCGATGCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGccc  >  2:75487/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                             CATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCC  >  NC_000913/3084688‑3084973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑