Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,329	T→G	I159S (ATT→AGT)	yhaB →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,329	0	T	G	100.0%	207.9 / NA	54	I159S (ATT→AGT)	yhaB	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/33);  total (21/33)

CTTTTGGTGAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGATTTAGTCCGTCGAAAAATGAA  >  NC_000913/3268188‑3268476                                                                                                                                              |                                                                                                                                                    cTTTTGGTGAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTc                                                                                                                                               >  1:387201/1‑148 (MQ=255)  ttttGGTGAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTc                                                                                                                                               >  2:136663/1‑147 (MQ=255)    ttGGTGAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGAtt                                                                                                                                          <  1:136664/150‑1 (MQ=255)      ggTGAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGAttgtt                                                                                                                                       <  1:860961/151‑1 (MQ=255)       gTGAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGAttgttg                                                                                                                                      <  1:726052/151‑1 (MQ=255)         gAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTgg                                                                                                                                     <  2:276877/150‑1 (MQ=255)         gAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGc                                                                                                                                    >  1:184471/1‑151 (MQ=255)         gAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGc                                                                                                                                    >  1:124580/1‑151 (MQ=255)          aaCAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCa                                                                                                                                   <  1:331963/151‑1 (MQ=255)           aCAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCaa                                                                                                                                  <  1:671736/151‑1 (MQ=255)            cAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCaaa                                                                                                                                 <  1:23272/151‑1 (MQ=255)            cAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCaa                                                                                                                                  >  1:398540/1‑150 (MQ=255)             aaTGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAAt                                                                                                                                >  1:377494/1‑151 (MQ=255)             aaTGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAAt                                                                                                                                <  2:343181/151‑1 (MQ=255)                gTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATtc                                                                                                                              <  2:537750/150‑1 (MQ=255)                  aCTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATtctc                                                                                                                            <  2:142111/150‑1 (MQ=255)                  aCTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTctct                                                                                                                           <  2:179083/151‑1 (MQ=255)                     tGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCttt                                                                                                                         <  1:689510/150‑1 (MQ=255)                      gCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCtttt                                                                                                                        <  2:384519/150‑1 (MQ=255)                        tCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCttttttt                                                                                                                     >  2:865831/1‑151 (MQ=255)                             cGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCAccc                                                                                                                >  2:830783/1‑151 (MQ=255)                                  aTAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATc                                                                                                            >  2:602157/1‑150 (MQ=255)                                       aaaGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTta                                                                                                      <  2:214753/151‑1 (MQ=255)                                        aaGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTta                                                                                                      >  1:141059/1‑150 (MQ=255)                                                gAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGAtt                                                                                             <  2:435117/151‑1 (MQ=255)                                                  aTGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGAtttt                                                                                           <  2:834185/151‑1 (MQ=255)                                                    gTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGAttttt                                                                                          >  1:377508/1‑150 (MQ=255)                                                      aTTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTAt                                                                                        <  1:360066/150‑1 (MQ=255)                                                        tAAGGAGTCGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTg                                                                                      <  1:562315/150‑1 (MQ=255)                                                        tAAGGAGGCGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAAATCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTAt                                                                                        <  2:784192/148‑1 (MQ=255)                                                         aaGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGat                                                                                    <  1:660432/151‑1 (MQ=255)                                                          aGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGatt                                                                                   >  2:406378/1‑151 (MQ=255)                                                                cGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGAttatttat                                                                             <  2:742229/151‑1 (MQ=255)                                                                 gATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGAttatttat                                                                             >  2:827208/1‑150 (MQ=255)                                                                 gATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGAttatttat                                                                             >  2:738448/1‑150 (MQ=255)                                                                 gATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTtata                                                                            <  1:779380/151‑1 (MQ=255)                                                                             ttGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAaat                                                                 >  2:77058/1‑150 (MQ=255)                                                                              tgtgCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAaata                                                                >  2:468587/1‑150 (MQ=255)                                                                                      aTTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCt                                                       >  1:809411/1‑151 (MQ=255)                                                                                         cACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGt                                                     <  2:579660/150‑1 (MQ=255)                                                                                               aaaaGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTtcatca                                              <  2:168065/151‑1 (MQ=255)                                                                                               aaaaGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTtcatca                                              <  2:657146/151‑1 (MQ=255)                                                                                                  agaagCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAAtt                                           <  2:77456/151‑1 (MQ=255)                                                                                                     agCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTa                                       <  1:725948/151‑1 (MQ=255)                                                                                                      gCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGt                                        >  1:600555/1‑150 (MQ=255)                                                                                                       ccATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTaa                                      <  2:383670/151‑1 (MQ=255)                                                                                                           cTTAACCCCCCTTGTGCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGat                                  >  2:382283/1‑151 (MQ=255)                                                                                                                       tgtgCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTg                        <  2:903133/149‑1 (MQ=255)                                                                                                                       tgtgCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTGTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGa                       <  1:406387/150‑1 (MQ=255)                                                                                                                          gCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGAttt                    >  1:572993/1‑150 (MQ=255)                                                                                                                          gCACTTTAATAAAACCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGAttt                    <  1:187287/150‑1 (MQ=255)                                                                                                                                    aaaaCCAGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGATTTAGTCCGTCGa          <  2:540651/150‑1 (MQ=255)                                                                                                                                          aGCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGATTTAGTCCGTCGAAAaatg    >  2:828276/1‑150 (MQ=255)                                                                                                                                           gCAGTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGATTTAGTCCGTCGAAAaatgaa  <  2:66596/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    CTTTTGGTGAACAATGTACTTGCTCAGCCGTCAATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTTTAATAATGATTTTTATTGATTATTTATAAAGGATAAAATAATGTTCCCTGTTTCATCAATTGGTAATGATATTAGTAGTGATTTAGTCCGTCGAAAAATGAA  >  NC_000913/3268188‑3268476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑