Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,279	A→C	N289H (AAC→CAC)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,279	0	A	C	100.0%	202.7 / NA	53	N289H (AAC→CAC)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/23);  total (30/23)

CCTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACAT  >  NC_000913/3269135‑3269424                                                                                                                                                 |                                                                                                                                                  ccTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGaa                                                                                                                                             <  1:763943/151‑1 (MQ=255)   tGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGaatt                                                                                                                                           >  2:671633/1‑151 (MQ=255)   tGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGaatt                                                                                                                                           >  1:440776/1‑151 (MQ=255)     gagaCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGaattaa                                                                                                                                         <  1:865872/151‑1 (MQ=255)     gagaCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGaattaa                                                                                                                                         <  1:643609/151‑1 (MQ=255)     gagaCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGaatta                                                                                                                                          <  2:392594/150‑1 (MQ=255)        aCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGaattaaatt                                                                                                                                      >  2:481341/1‑151 (MQ=255)                  agaaCAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAAATAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATg                                                                                                                            >  2:712174/1‑151 (MQ=255)                    aaCAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGaa                                                                                                                          <  2:832666/151‑1 (MQ=255)                           aaaaaTTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATtc                                                                                                                   >  1:658608/1‑151 (MQ=255)                            aaaaTTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATtct                                                                                                                  >  2:607772/1‑151 (MQ=255)                             aaaTTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATtctc                                                                                                                 <  1:695867/151‑1 (MQ=255)                                  aTGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCtcatc                                                                                                              >  1:535601/1‑149 (MQ=255)                                    gagaTTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGaa                                                                                                          <  2:854122/151‑1 (MQ=255)                                    gagaTTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGaa                                                                                                          <  1:786613/151‑1 (MQ=255)                                    gagaTTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGa                                                                                                           >  1:587217/1‑150 (MQ=255)                                     agaTTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAGAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGaaa                                                                                                         <  2:43132/151‑1 (MQ=255)                                     agaTTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGaa                                                                                                          >  2:683536/1‑150 (MQ=255)                                            cAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAatat                                                                                                   >  2:306587/1‑150 (MQ=255)                                             aaTTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAatatat                                                                                                 <  2:415857/151‑1 (MQ=255)                                                  ttaaaTCTATTCCAAGGGCAACTCTTAAAGATAAAATAGAAAATGAAGTAAAAAAAGAAATATTTAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGAtt                                                                                             <  1:165747/148‑1 (MQ=255)                                                    aaaTCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTAc                                                                                           >  1:2434/1‑150 (MQ=255)                                                     aaTCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACAt                                                                                         <  2:197248/151‑1 (MQ=255)                                                         tataCCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGat                                                                                     >  1:857163/1‑151 (MQ=255)                                                          atatCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGata                                                                                    <  2:407846/151‑1 (MQ=255)                                                          ataCCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATc                                                                                        <  1:329580/147‑1 (MQ=255)                                                          ataCCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATc                                                                                        <  1:124234/147‑1 (MQ=255)                                                            aCCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGatat                                                                                   >  2:166034/1‑150 (MQ=255)                                                                aGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATc                                                                               >  1:254466/1‑150 (MQ=255)                                                                  ggCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCAtt                                                                            <  1:173994/151‑1 (MQ=255)                                                                     aaCTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGaa                                                                         <  2:735722/151‑1 (MQ=255)                                                                     aaCTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGa                                                                          >  2:592364/1‑150 (MQ=255)                                                                              aGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGGTTACATCGATATAATCATTGAAGATTATGa                                                                 >  2:475792/1‑150 (MQ=255)                                                                                aTAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGaaa                                                               >  2:419930/1‑150 (MQ=255)                                                                                     ttAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATAcc                                                          >  1:802504/1‑150 (MQ=255)                                                                                         aaaaTGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGa                                                     <  1:625366/151‑1 (MQ=255)                                                                                                aTTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCt                                              <  1:851167/151‑1 (MQ=255)                                                                                                  tAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCtt                                             >  1:122607/1‑150 (MQ=255)                                                                                                   aaCAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTAt                                           >  2:236920/1‑151 (MQ=255)                                                                                                   aaCAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTAt                                           <  2:224771/151‑1 (MQ=255)                                                                                                         aGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGgtc                                     <  2:791634/151‑1 (MQ=255)                                                                                                                 ataAAAGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTccccc                              >  1:68322/1‑150 (MQ=255)                                                                                                                   aaaaGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCaa                            >  1:901367/1‑150 (MQ=255)                                                                                                                    aaaGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAaat                          >  1:223687/1‑151 (MQ=255)                                                                                                                    aaaGCTCATCGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAaat                          >  1:863566/1‑151 (MQ=255)                                                                                                                             cGAAAATCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATAc                 <  2:434360/151‑1 (MQ=255)                                                                                                                                aaaTCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTa               >  2:624151/1‑150 (MQ=255)                                                                                                                                aaaTCATCAATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTAt              >  2:438826/1‑151 (MQ=255)                                                                                                                                       caATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAttt        >  1:564707/1‑150 (MQ=255)                                                                                                                                       caATTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAttt        >  1:607940/1‑150 (MQ=255)                                                                                                                                        aaTTGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTa       >  1:73077/1‑150 (MQ=255)                                                                                                                                           tGCTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAAc    <  1:758942/150‑1 (MQ=255)                                                                                                                                             cTTGCACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACAt  >  2:568244/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CCTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACAT  >  NC_000913/3269135‑3269424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑