Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,343,061	T→G	S76A (TCG→GCG)	lptC →	periplasmic membrane‑anchored LPS‑binding protein; LPS export protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,343,061	0	T	G	100.0%	198.8 / NA	54	S76A (TCG→GCG)	lptC	periplasmic membrane‑anchored LPS‑binding protein; LPS export protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/27);  total (27/27)

AAAAGACGATACCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGA  >  NC_000913/3342913‑3343203                                                                                                                                                     |                                                                                                                                               aaaaGACGATACCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCg                                                                                                                                              >  1:831959/1‑151 (MQ=255)  aaaGACGATACCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGt                                                                                                                                             >  1:849756/1‑151 (MQ=255)      aCGATACCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGtt                                                                                                                                         >  2:330438/1‑151 (MQ=255)       cGATACCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGttt                                                                                                                                        >  1:759625/1‑151 (MQ=255)       cGATACCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGttt                                                                                                                                        >  1:65537/1‑151 (MQ=255)           aCCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGc                                                                                                                                    <  1:807410/151‑1 (MQ=255)                     tggtCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACt                                                                                                                          >  2:438156/1‑151 (MQ=255)                      ggtCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACt                                                                                                                          <  1:161503/150‑1 (MQ=255)                         cgtcAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTAcc                                                                                                                      >  1:181052/1‑151 (MQ=255)                         cgtcAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTAcc                                                                                                                      >  1:399076/1‑151 (MQ=255)                            caacaacaaTGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCAc                                                                                                                    <  1:659357/150‑1 (MQ=255)                            caacaacaaTGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACg                                                                                                                   <  1:885071/151‑1 (MQ=255)                                   aaTGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATa                                                                                                            <  2:223526/151‑1 (MQ=255)                                                ataAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATcc                                                                                                <  2:302841/150‑1 (MQ=255)                                                 taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCg                                                                                              >  1:803633/1‑151 (MQ=255)                                                   aaaGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATACAATCCCg                                                                                              >  1:600516/1‑149 (MQ=255)                                                    aaGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGAc                                                                                            >  2:48782/1‑150 (MQ=255)                                                       cGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATgg                                                                                        >  2:639571/1‑151 (MQ=255)                                                         aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATg                                                                                         <  1:116797/148‑1 (MQ=255)                                                         aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTc                                                                                      <  1:262562/151‑1 (MQ=255)                                                         aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTc                                                                                      <  1:699559/151‑1 (MQ=255)                                                          gCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTcc                                                                                     <  1:537017/151‑1 (MQ=255)                                                               cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTaaa                                                                                <  1:477309/151‑1 (MQ=255)                                                                ggACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTaaa                                                                                >  2:327307/1‑150 (MQ=255)                                                                   cacGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCa                                                                            <  1:710664/151‑1 (MQ=255)                                                                    acGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAg                                                                           <  1:404808/151‑1 (MQ=255)                                                                     cGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGc                                                                             >  1:299605/1‑148 (MQ=255)                                                                           tCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGCGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGCCCGTAAAAGCAGATaaa                                                                      >  1:245029/1‑149 (MQ=255)                                                                              ataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGcc                                                                   <  1:26550/149‑1 (MQ=255)                                                                                  cccAGAAGGGGCGCTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCt                                                              <  2:219874/150‑1 (MQ=255)                                                                                  cccAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTg                                                             <  1:767343/151‑1 (MQ=255)                                                                                      gAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCa                                                         <  2:298001/151‑1 (MQ=255)                                                                                        aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAAt                                                       <  1:571676/151‑1 (MQ=255)                                                                                             cACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCg                                                  <  2:659159/151‑1 (MQ=255)                                                                                                tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGAt                                               >  1:521895/1‑151 (MQ=255)                                                                                                 aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGAt                                               <  1:690693/150‑1 (MQ=255)                                                                                                            tgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTtat                                    <  2:537795/150‑1 (MQ=255)                                                                                                             gattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTatat                                  <  2:124149/151‑1 (MQ=255)                                                                                                             gattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTatat                                  <  2:260411/151‑1 (MQ=255)                                                                                                             gattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTatat                                  >  2:465828/1‑151 (MQ=255)                                                                                                                      acacGTTGAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGtt                         >  1:417166/1‑151 (MQ=255)                                                                                                                             gAATATTATTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTc                   >  1:780709/1‑150 (MQ=255)                                                                                                                                tattatTCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACg               >  2:779565/1‑151 (MQ=255)                                                                                                                                   tattCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCa             <  2:279823/150‑1 (MQ=255)                                                                                                                                    attCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACt           >  1:281131/1‑151 (MQ=255)                                                                                                                                      tCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACtt          <  2:399067/150‑1 (MQ=255)                                                                                                                                      tCCGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTtg         >  1:414041/1‑151 (MQ=255)                                                                                                                                       ccGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTtgt        <  2:481130/151‑1 (MQ=255)                                                                                                                                        cGATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTtgtg       >  2:537425/1‑151 (MQ=255)                                                                                                                                         gATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGc      >  2:410350/1‑151 (MQ=255)                                                                                                                                         gATCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGc      >  1:778866/1‑151 (MQ=255)                                                                                                                                           tCAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGcc     >  1:625538/1‑150 (MQ=255)                                                                                                                                            cAGGCCGTTGCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAg   <  2:750734/151‑1 (MQ=255)                                                                                                                                               gCCGTTGCGTGGATTACGCAGCCGGTACTTACCACGGTTGATAAGGATAAAATCCCGACATGGTCCGTAACAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGa  >  2:342239/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                               AAAAGACGATACCGCCCAGGTGGTCGTCAACAACAATGATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCACGTTTGATAAGGATAAAATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGA  >  NC_000913/3342913‑3343203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑