Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,350,729	T→G	E766A (GAA→GCA)	arcB ←	aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,350,729	0	T	G	100.0%	161.6 / NA	43	E766A (GAA→GCA)	arcB	aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/19);  total (24/19)

GGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTAT  >  NC_000913/3350581‑3350876                                                                                                                                                     |                                                                                                                                                    ggCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCt                                                                                                                                                   <  2:695599/151‑1 (MQ=255)        aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTAcgtcgt                                                                                                                                            <  1:465978/151‑1 (MQ=255)           aCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGAc                                                                                                                                         >  2:916340/1‑151 (MQ=255)                  tgtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCActc                                                                                                                                  >  2:402897/1‑151 (MQ=255)                       ccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTcttctt                                                                                                                             <  1:751970/151‑1 (MQ=255)                       ccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTcttctt                                                                                                                             <  1:519541/151‑1 (MQ=255)                           gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCAt                                                                                                                         >  1:697400/1‑151 (MQ=255)                             aaCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCAtct                                                                                                                       >  2:515181/1‑151 (MQ=255)                             aaCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCACTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCAtct                                                                                                                       >  2:807964/1‑151 (MQ=255)                              aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCAtctc                                                                                                                      <  2:270215/151‑1 (MQ=255)                                  atcatAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTc                                                                                                                   >  1:472216/1‑150 (MQ=255)                                              tACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCg                                                                                                      >  1:330261/1‑151 (MQ=255)                                               aCGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCg                                                                                                      <  1:877739/150‑1 (MQ=255)                                                 ggCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCC‑GGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGt                                                                                                  <  1:644434/151‑1 (MQ=255)                                                         cAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTc                                                                                           >  2:112130/1‑151 (MQ=255)                                                                      gtgtTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAgg                                                                              >  1:714430/1‑151 (MQ=255)                                                                       tgtTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGt                                                                             >  1:90034/1‑151 (MQ=255)                                                                              gcAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAgg                                                                         >  1:467494/1‑148 (MQ=255)                                                                                aGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACt                                                                    >  1:856518/1‑151 (MQ=255)                                                                                      cgcgCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAAttt                                                              >  1:584819/1‑151 (MQ=255)                                                                                       gcgcACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTg                                                             <  1:477995/151‑1 (MQ=255)                                                                                            ccccGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGCCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGAc                                                        >  1:403976/1‑151 (MQ=255)                                                                                                     aGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCt                                                  <  1:601303/148‑1 (MQ=255)                                                                                                      gCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTg                                              >  1:871757/1‑151 (MQ=255)                                                                                                       ccGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGc                                             <  1:451146/151‑1 (MQ=255)                                                                                                       ccGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGc                                             >  1:100388/1‑151 (MQ=255)                                                                                                       ccGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGc                                             >  2:138916/1‑151 (MQ=255)                                                                                                                 tttttAGTGGCTTTTGTCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGt                                    <  2:27936/150‑1 (MQ=255)                                                                                                                  ttttAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTa                                   >  2:214699/1‑150 (MQ=255)                                                                                                                    ttAGTGGCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAAc                                 <  2:918754/150‑1 (MQ=255)                                                                                                                         ggCTTTTGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCAc                            <  1:364683/150‑1 (MQ=255)                                                                                                                            ttttGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAc                        >  1:579677/1‑151 (MQ=255)                                                                                                                             tttGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcc                       >  1:432327/1‑151 (MQ=255)                                                                                                                              ttGCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcc                       <  2:108838/150‑1 (MQ=255)                                                                                                                                gCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                     <  2:472204/150‑1 (MQ=255)                                                                                                                                gCCACCCACGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                     <  1:515195/150‑1 (MQ=255)                                                                                                                                 ccacccacGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgc                   >  2:145472/1‑151 (MQ=255)                                                                                                                                 ccacccacGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgc                   <  1:807982/151‑1 (MQ=255)                                                                                                                                  cacccacGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccacc                  <  2:425346/151‑1 (MQ=255)                                                                                                                                       acGCTTTCAGCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCAcc             >  1:563095/1‑151 (MQ=255)                                                                                                                                           tttCAGCACTGCTCCGTCGTGCCGCCATTCTTCTTCCATCTCTTCAACCCTTCCCCCGACTTTATCTCCCCAGGCGGGAAGGTCGGGAGACTAAATTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCtt           <  1:630894/149‑1 (MQ=255)                                                                                                                                                gCACTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAAtttt    >  1:738033/1‑151 (MQ=255)                                                                                                                                                  aCTGCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTAt  >  1:548490/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTAT  >  NC_000913/3350581‑3350876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑