Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,383,818	T→G	I151L (ATC→CTC)	mdh ←	malate dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,383,818	0	T	G	100.0%	231.5 / NA	60	I151L (ATC→CTC)	mdh	malate dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/25);  total (35/25)

GCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGT  >  NC_000913/3383670‑3383963                                                                                                                                                     |                                                                                                                                                  gCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGagat                                                                                                                                                 <  1:824984/151‑1 (MQ=255)   cACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATcc                                                                                                                                               >  1:179591/1‑151 (MQ=255)   cACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATc                                                                                                                                                >  1:725742/1‑150 (MQ=255)     cTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATcc                                                                                                                                               >  1:880483/1‑149 (MQ=255)         cTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTg                                                                                                                                         >  1:29095/1‑151 (MQ=255)                gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGc                                                                                                                                  >  1:451235/1‑151 (MQ=255)                       tAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAAc                                                                                                                             >  1:310429/1‑149 (MQ=255)                         aCGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAgtt                                                                                                                         >  1:558797/1‑151 (MQ=255)                             cAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAgtttgtt                                                                                                                     <  1:522939/151‑1 (MQ=255)                              aGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAgtttgtt                                                                                                                     <  2:205418/150‑1 (MQ=255)                              aGGAACCTGTGACAGCAGCGGCAGAATGGCAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAgtttgttt                                                                                                                    >  1:809717/1‑151 (MQ=255)                                gAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGttttt                                                                                                                  >  1:111055/1‑151 (MQ=255)                                 aaCCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTg                                                                                                                 <  1:889302/151‑1 (MQ=255)                                   ccTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTc                                                                                                               <  1:474408/151‑1 (MQ=255)                                   ccTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTc                                                                                                               >  2:345525/1‑151 (MQ=255)                                    cTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTc                                                                                                               >  2:36188/1‑150 (MQ=255)                                     tgtgACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCAt                                                                                                             >  1:755432/1‑151 (MQ=255)                                      gtgACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCAt                                                                                                             <  1:624887/150‑1 (MQ=255)                                         aCAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAac                                                                                                         <  1:452025/151‑1 (MQ=255)                                            gcagcGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACAcc                                                                                                      <  2:293031/151‑1 (MQ=255)                                               gcGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGc                                                                                                   <  2:419318/151‑1 (MQ=255)                                                cGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCt                                                                                                  >  1:179436/1‑151 (MQ=255)                                                    aGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCttttt                                                                                              <  1:566475/151‑1 (MQ=255)                                                    aGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCttttt                                                                                              >  2:613063/1‑151 (MQ=255)                                                     gAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCtttttt                                                                                             <  1:614979/151‑1 (MQ=255)                                                      aaTGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGGACAGTTTGTTTTTGTCATAAACACCGGCtttttt                                                                                             <  2:611671/150‑1 (MQ=255)                                                      aaTGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTc                                                                                            >  1:49770/1‑151 (MQ=255)                                                          gTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGc                                                                                         >  2:847241/1‑150 (MQ=255)                                                            aaCACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCAc                                                                                       <  1:668341/150‑1 (MQ=255)                                                            aaCACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACt                                                                                      >  1:63452/1‑151 (MQ=255)                                                              cacCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTc                                                                                    >  2:769745/1‑151 (MQ=255)                                                               acCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTc                                                                                    >  1:25021/1‑150 (MQ=255)                                                                  agagTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTcagca                                                                                <  1:176203/151‑1 (MQ=255)                                                                    agTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCagcagc                                                                              >  1:313893/1‑151 (MQ=255)                                                                       gACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAAt                                                                           >  2:531624/1‑151 (MQ=255)                                                                       gACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAAt                                                                           <  1:322059/151‑1 (MQ=255)                                                                             cAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAAc                                                                     <  1:470193/151‑1 (MQ=255)                                                                                 aaCCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtgtg                                                                 >  2:109317/1‑151 (MQ=255)                                                                                   ccGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTgtggt                                                               <  2:450205/151‑1 (MQ=255)                                                                                       cACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGtt                                                            >  2:671829/1‑150 (MQ=255)                                                                                            cAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCgg                                                      <  2:179433/151‑1 (MQ=255)                                                                                             aaCTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCggg                                                     >  2:555621/1‑151 (MQ=255)                                                                                             aaCTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCggg                                                     >  2:577568/1‑151 (MQ=255)                                                                                             aaCTTCACCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCggg                                                     >  2:226660/1‑151 (MQ=255)                                                                                                   gCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTg                                               >  1:199165/1‑151 (MQ=255)                                                                                                       ggCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGataa                                           <  2:302635/151‑1 (MQ=255)                                                                                                         cTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGataat                                          >  1:158789/1‑150 (MQ=255)                                                                                                            tttGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATAc                                        <  2:23914/149‑1 (MQ=255)                                                                                                              tGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGCGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCaa                                     >  2:561858/1‑150 (MQ=255)                                                                                                                   ttCAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCa                                 <  2:73726/149‑1 (MQ=255)                                                                                                                     cAGTTCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCt                             <  2:212856/151‑1 (MQ=255)                                                                                                                        ttCCGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCttt                           >  2:872777/1‑150 (MQ=255)                                                                                                                          ccGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATTCCAATGCACGCTTTct                         <  2:448984/150‑2 (MQ=255)                                                                                                                           cGCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCggg                       >  1:316460/1‑151 (MQ=255)                                                                                                                            gCAACAAAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGc                      <  2:89121/151‑1 (MQ=255)                                                                                                                               acaaAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAgg                   >  1:859891/1‑151 (MQ=255)                                                                                                                                caaAGGTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGt                  >  1:338672/1‑151 (MQ=255)                                                                                                                                    ggTGTTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTc              <  2:208288/151‑1 (MQ=255)                                                                                                                                       gtTGGAACGAATGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGc            >  1:441547/1‑150 (MQ=255)                                                                                                                                                aaTGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGt  <  2:725724/151‑1 (MQ=255)                                                                                                                                                 aTGAGATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTg   >  2:10053/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGT  >  NC_000913/3383670‑3383963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑