Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,392,795	A→C	I1155S (ATT→AGT)	yhdP ←	DUF3971‑AsmA2 domains protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,392,795	0	A	C	100.0%	209.9 / NA	55	I1155S (ATT→AGT)	yhdP	DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/28);  total (27/28)

AGAAATCTCTGGTGCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGT  >  NC_000913/3392647‑3392940                                                                                                                                                     |                                                                                                                                                  aGAAATCTCTGGTGCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTg                                                                                                                                                 >  2:457409/1‑151 (MQ=255)   aaaTCTCTGGTGCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGa                                                                                                                                               <  1:263592/151‑1 (MQ=255)           ggTGCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAAt                                                                                                                                       <  2:26630/151‑1 (MQ=255)            gTGCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATa                                                                                                                                      <  2:147032/151‑1 (MQ=255)              gCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAAt                                                                                                                                       >  1:97277/1‑148 (MQ=255)              gCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGa                                                                                                                                    <  1:328565/151‑1 (MQ=255)                gACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAAc                                                                                                                                  >  2:227836/1‑151 (MQ=255)                gACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAAc                                                                                                                                  <  1:532875/151‑1 (MQ=255)                    aCCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCtt                                                                                                                              <  2:848093/151‑1 (MQ=255)                       gCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGc                                                                                                                           >  1:447296/1‑151 (MQ=255)                           ccATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCaaa                                                                                                                       <  1:48272/151‑1 (MQ=255)                             atatTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAg                                                                                                                     <  2:737448/151‑1 (MQ=255)                              tatTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGCGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAACgt                                                                                                                    >  2:46268/1‑151 (MQ=255)                               atTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAgtg                                                                                                                   >  2:666435/1‑151 (MQ=255)                                    ggTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAACATAGAACCCTTCGCCAAAAGTGtctc                                                                                                               <  2:87689/150‑1 (MQ=255)                                     gTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTg                                                                                                             <  2:580838/151‑1 (MQ=255)                                           aCGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATc                                                                                                        >  2:195/1‑150 (MQ=255)                                             gTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCaa                                                                                                      >  1:445536/1‑150 (MQ=255)                                               aCCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAAc                                                                                                   >  1:75742/1‑151 (MQ=255)                                               aCCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAAc                                                                                                   >  2:71245/1‑151 (MQ=255)                                                   gATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAg                                                                                               <  2:47440/151‑1 (MQ=255)                                                       tACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCtt                                                                                            >  2:596940/1‑150 (MQ=255)                                                        aCCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTAc                                                                                          >  2:351574/1‑151 (MQ=255)                                                          cGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGc                                                                                        >  2:239752/1‑151 (MQ=255)                                                          cGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTATAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGc                                                                                        <  1:804411/151‑1 (MQ=255)                                                           gACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCa                                                                                       <  1:712829/151‑1 (MQ=255)                                                            aCCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCAt                                                                                      >  2:492622/1‑151 (MQ=255)                                                                 ttCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAgg                                                                                  <  1:878954/150‑1 (MQ=255)                                                                              ccGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCa                                                                     <  2:836689/150‑1 (MQ=255)                                                                              ccGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCa                                                                     <  1:226034/150‑1 (MQ=255)                                                                                    ccAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAgc                                                               <  2:300915/150‑1 (MQ=255)                                                                                          cacccaccaGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCAACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCgcagca                                                         >  1:646704/4‑150 (MQ=255)                                                                                          cATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCgcagca                                                         >  1:646718/1‑150 (MQ=255)                                                                                           aTCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAAt                                                       >  2:130576/1‑151 (MQ=255)                                                                                             ccaccaGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTg                                                     <  1:162567/151‑1 (MQ=255)                                                                                                     gtgtCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCAt                                             >  2:266032/1‑151 (MQ=255)                                                                                                           tcgGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCgg                                       >  2:345961/1‑151 (MQ=255)                                                                                                             ggTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTa                                     <  2:204495/151‑1 (MQ=255)                                                                                                                gtgCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTg                                  <  2:284206/151‑1 (MQ=255)                                                                                                                gtgCATAACGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTg                                  >  1:219341/1‑151 (MQ=255)                                                                                                                      aaCGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTc                             <  2:359246/150‑1 (MQ=255)                                                                                                                       aCGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCgg                           >  2:22856/1‑151 (MQ=255)                                                                                                                        cGCCGTCTTTAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGt                          <  2:781257/151‑1 (MQ=255)                                                                                                                                ttAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                     <  1:224300/148‑1 (MQ=255)                                                                                                                                 tAATCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGcc                 >  1:866099/1‑151 (MQ=255)                                                                                                                                   aTCCACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCtt               >  1:147471/1‑151 (MQ=255)                                                                                                                                     ccACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCttt              <  2:61135/150‑1 (MQ=255)                                                                                                                                      cACGCGGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTAc            <  2:331814/151‑1 (MQ=255)                                                                                                                                        cgcgGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTAccc          <  2:646703/151‑1 (MQ=255)                                                                                                                                        cgcgGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTAccc          <  2:646689/151‑1 (MQ=255)                                                                                                                                        cgcgGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTAccc          >  2:133008/1‑151 (MQ=255)                                                                                                                                          cgGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTAccc          >  1:179947/1‑149 (MQ=255)                                                                                                                                          cgGTGCTGCGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAg        <  2:487821/151‑1 (MQ=255)                                                                                                                                                 gcGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGt  >  1:482362/1‑150 (MQ=255)                                                                                                                                                 gcGACTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGt  >  1:395851/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AGAAATCTCTGGTGCGACAACCGCTTCCATATTCAGGTCGCGACGTACCAGATTTACCGACCCTTTCATGGCGATATCCGCCTCCAGGCCATCCACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGT  >  NC_000913/3392647‑3392940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑