Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,468,693 T→G T387T (ACA→ACC)  chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,468,6930TG98.3% 212.3 / ‑4.6 60T387T (ACA→ACC) chiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (30/29);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCAC  >  NC_000913/3468547‑3468837
                                                                                                                                                  |                                                                                                                                                
gTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTAc                                                                                                                                              <  2:94292/151‑1 (MQ=255)
gTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTAc                                                                                                                                              >  1:436184/1‑151 (MQ=255)
       aaTGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCgag                                                                                                                                       <  1:242670/151‑1 (MQ=255)
        aTGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCgaga                                                                                                                                      <  2:79238/151‑1 (MQ=255)
         tgtgCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGAc                                                                                                                                     >  1:522787/1‑151 (MQ=255)
              aTCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCAt                                                                                                                                >  1:36266/1‑151 (MQ=255)
                cGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATgg                                                                                                                              >  2:639432/1‑151 (MQ=255)
                cGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATg                                                                                                                               <  1:833454/150‑1 (MQ=255)
                     taATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTAt                                                                                                                         >  2:735146/1‑151 (MQ=255)
                        tCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTgg                                                                                                                      >  1:522916/1‑151 (MQ=255)
                              aTGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGtttt                                                                                                                >  2:725171/1‑151 (MQ=255)
                                 cTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCt                                                                                                                                           <  2:161823/121‑1 (MQ=255)
                                 cTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCt                                                                                                                                           >  1:161823/1‑121 (MQ=255)
                                  tGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCa                                                                                                              >  2:609722/1‑149 (MQ=255)
                                  tGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAgc                                                                                                            >  1:503802/1‑151 (MQ=255)
                                  tGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAgc                                                                                                            >  1:729541/1‑151 (MQ=255)
                                      cgcgCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTg                                                                                                        <  1:407802/151‑1 (MQ=255)
                                         gcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACACTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGt                                                                                                      >  2:848865/1‑150 (MQ=255)
                                            gTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTAcc                                                                                                   <  2:539467/150‑1 (MQ=255)
                                              aCGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCg                                                                                                <  1:39822/151‑1 (MQ=255)
                                                      cTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAAt                                                                                        >  1:750033/1‑151 (MQ=255)
                                                       tGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAAt                                                                                        >  1:837038/1‑150 (MQ=255)
                                                       tGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATc                                                                                       >  2:631262/1‑151 (MQ=255)
                                                       tGGCATGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGCTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGCGGGCAATc                                                                                       >  2:801407/1‑151 (MQ=255)
                                                        ggCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATc                                                                                       <  1:796653/150‑1 (MQ=255)
                                                        ggCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCt                                                                                      <  2:256611/151‑1 (MQ=255)
                                                          acTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCttt                                                                                    <  1:613555/150‑1 (MQ=255)
                                                              gAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTGCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTTCCCCGTGGGCAATCTTTAg                                                                                  <  2:504790/149‑1 (MQ=255)
                                                               aaaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGacc                                                                               >  1:733626/1‑151 (MQ=255)
                                                                aaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGaccc                                                                              <  2:608939/151‑1 (MQ=255)
                                                                   cATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGacccaac                                                                           <  1:782733/151‑1 (MQ=255)
                                                                        gCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACccacc                                                                      <  2:745814/151‑1 (MQ=255)
                                                                            caaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGc                                                                  <  1:124422/151‑1 (MQ=255)
                                                                            caaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGCTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGc                                                                  >  2:528760/1‑151 (MQ=255)
                                                                                gACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGTCTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGtt                                                               <  1:795557/150‑1 (MQ=255)
                                                                                gACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTc                                                              <  1:251198/151‑1 (MQ=255)
                                                                                 aCGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCt                                                             >  1:716694/1‑151 (MQ=255)
                                                                                     cgccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGt                                                          >  1:176291/1‑150 (MQ=255)
                                                                                      gccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCCGGCGTTCTTGTa                                                         >  1:521390/1‑150 (MQ=255)
                                                                                           tATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACAt                                                    <  2:714310/150‑1 (MQ=255)
                                                                                            aTCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACAtt                                                   <  2:226856/150‑1 (MQ=255)
                                                                                            aTCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTa                                                  <  1:387724/151‑1 (MQ=255)
                                                                                             tCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTAc                                                 >  1:116933/1‑151 (MQ=255)
                                                                                                      caGGTAGTCGGGTTACCGTACTGACTCAATTCTGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGaaaa                                        <  1:888299/151‑1 (MQ=255)
                                                                                                         gTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATaa                                     <  2:697827/151‑1 (MQ=255)
                                                                                                                   tACCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAt                           >  1:288258/1‑151 (MQ=255)
                                                                                                                    aCCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAtt                          >  1:715942/1‑151 (MQ=255)
                                                                                                                    aCCGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGAAGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTGCCATTGAAAATAGCATGGTAAAt                           <  2:810747/150‑1 (MQ=255)
                                                                                                                     ccGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                         >  1:45890/1‑151 (MQ=255)
                                                                                                                     ccGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                         <  2:433727/151‑1 (MQ=255)
                                                                                                                     ccGTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAtt                          <  1:633661/150‑1 (MQ=255)
                                                                                                                       gTACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                         >  1:434410/1‑149 (MQ=255)
                                                                                                                        tACTGACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGc                      >  1:916175/1‑151 (MQ=255)
                                                                                                                            gACTCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTAcc                  <  2:369127/151‑1 (MQ=255)
                                                                                                                               tCAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTc               >  1:854208/1‑151 (MQ=255)
                                                                                                                                cAATTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTcc              >  1:770545/1‑151 (MQ=255)
                                                                                                                                  aTTCCGCAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTg            >  2:123907/1‑151 (MQ=255)
                                                                                                                                        cAGCGGTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCt      <  1:460782/151‑1 (MQ=255)
                                                                                                                                            ggTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCAc  <  2:593736/151‑1 (MQ=255)
                                                                                                                                            ggTAGCGGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCAc  >  1:249546/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCAC  >  NC_000913/3468547‑3468837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑