Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 348,568 A→G intergenic (‑125/‑114) prpR ← / → prpB propionate catabolism operon regulatory protein/2‑methylisocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913348,5680AG100.0% 255.4 / NA 67intergenic (‑125/‑114)prpR/prpBpropionate catabolism operon regulatory protein/2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/40);  total (27/40)

TTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCG  >  NC_000913/348421‑348713
                                                                                                                                                   |                                                                                                                                                 
ttAAGCCGTGCTGGATGTGCCATAGCGCACCGCACAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGAGTAGTTCATGGCtt                                                                                                                                                >  2:175231/1‑151 (MQ=255)
 tAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCtt                                                                                                                                                <  1:527141/150‑1 (MQ=255)
  aaGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCttt                                                                                                                                               >  2:392490/1‑150 (MQ=255)
  aaGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTa                                                                                                                                              <  1:142773/151‑1 (MQ=255)
     ccGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTAtct                                                                                                                                           <  1:46077/151‑1 (MQ=255)
           tggATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAAc                                                                                                                                      >  1:636481/1‑150 (MQ=255)
             gATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACaaa                                                                                                                                   <  2:457710/151‑1 (MQ=255)
               tgtgCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAAtt                                                                                                                                 >  1:767415/1‑151 (MQ=255)
                         cgcACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTaaa                                                                                                                        <  2:314465/150‑1 (MQ=255)
                         cgcACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAAc                                                                                                                       >  2:777838/1‑151 (MQ=255)
                          gcACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACa                                                                                                                      <  1:297611/151‑1 (MQ=255)
                          gcACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACa                                                                                                                      <  2:816789/151‑1 (MQ=255)
                            aCCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACAtt                                                                                                                    <  1:557193/151‑1 (MQ=255)
                             ccGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACAttt                                                                                                                   <  1:241558/151‑1 (MQ=255)
                             ccGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACAttt                                                                                                                   <  1:306740/151‑1 (MQ=255)
                              cGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTa                                                                                                                  <  2:924250/151‑1 (MQ=255)
                               gCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTaa                                                                                                                 <  1:575493/151‑1 (MQ=255)
                               gCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTa                                                                                                                  >  2:71596/1‑150 (MQ=255)
                                cAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAAt                                                                                                                <  2:244355/151‑1 (MQ=255)
                                 aaaGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAAtt                                                                                                               <  2:654677/151‑1 (MQ=255)
                                  aaGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAAttt                                                                                                              >  2:137011/1‑151 (MQ=255)
                                    gTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAAtttt                                                                                                             >  1:88416/1‑150 (MQ=255)
                                    gTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTtta                                                                                                            <  2:850407/151‑1 (MQ=255)
                                     ttAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTtta                                                                                                            <  2:587688/150‑1 (MQ=255)
                                        aGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTttatta                                                                                                         >  2:639642/1‑150 (MQ=255)
                                          aaaCCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAgg                                                                                                      >  1:805604/1‑151 (MQ=255)
                                          aaaCCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAgg                                                                                                      <  2:864257/151‑1 (MQ=255)
                                           aaCCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGc                                                                                                     <  1:427297/151‑1 (MQ=255)
                                              cGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAAt                                                                                                  <  1:134365/151‑1 (MQ=255)
                                              cGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAAt                                                                                                  >  2:297082/1‑151 (MQ=255)
                                                 atatTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATtgt                                                                                               <  1:135454/151‑1 (MQ=255)
                                                 atatTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATtgt                                                                                               >  2:606329/1‑151 (MQ=255)
                                                  tatTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATtgtg                                                                                              >  1:489335/1‑151 (MQ=255)
                                                    ttGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGc                                                                                            >  1:505179/1‑151 (MQ=255)
                                                        gTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCacac                                                                                        >  2:512098/1‑151 (MQ=255)
                                                                 tGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTTAAACGCATATTTGCGGATTAGTTCATGGCTTTAACTCTTACAAAATGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTg                                                                                  <  2:788188/148‑1 (MQ=255)
                                                                 tGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTg                                                                                  <  1:354413/148‑1 (MQ=255)
                                                                   tttCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCttt                                                                              <  1:657881/150‑1 (MQ=255)
                                                                          aTTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTATGGCAATTGTGGCACACCCCTTGCTTTGTCTTTaa                                                                      <  2:405594/151‑2 (MQ=255)
                                                                                gCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGc                                                                >  1:591788/1‑151 (MQ=255)
                                                                                  aaTGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCaa                                                              <  2:466330/151‑1 (MQ=255)
                                                                                   aTGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCaaa                                                             <  2:797425/151‑1 (MQ=255)
                                                                                        aCGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACa                                                        <  1:583242/151‑1 (MQ=255)
                                                                                          gcgGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACaa                                                       <  2:690830/150‑1 (MQ=255)
                                                                                                aaaCATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGAt                                                 >  2:838179/1‑150 (MQ=255)
                                                                                                aaaCATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATa                                                <  2:36983/151‑1 (MQ=255)
                                                                                                  aCATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAAc                                              <  1:23446/151‑1 (MQ=255)
                                                                                                   cATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACa                                             <  1:333906/151‑1 (MQ=255)
                                                                                                        ccTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTAATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAgg                                         >  2:273153/1‑150 (MQ=255)
                                                                                                         cTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGAt                                       >  2:590866/1‑151 (MQ=255)
                                                                                                            aaaCGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATggg                                    >  2:438953/1‑151 (MQ=255)
                                                                                                             aaCGTTAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGc                                   <  2:747864/151‑1 (MQ=255)
                                                                                                                 ttAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTAt                                <  2:767397/150‑1 (MQ=255)
                                                                                                                 ttAACTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATg                               >  2:123338/1‑151 (MQ=255)
                                                                                                                   aaCTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGtc                             >  2:538534/1‑151 (MQ=255)
                                                                                                                    aCTGAAACGCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGtct                            <  1:86024/151‑1 (MQ=255)
                                                                                                                            gCATATTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACActc                    >  1:454231/1‑151 (MQ=255)
                                                                                                                                atTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTcc                 >  2:337270/1‑150 (MQ=255)
                                                                                                                                atTTGCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCa                <  2:865763/151‑1 (MQ=255)
                                                                                                                                    gCGGATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGt             >  2:828879/1‑150 (MQ=255)
                                                                                                                                       gATTAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTaa           >  2:21653/1‑149 (MQ=255)
                                                                                                                                          tAGTTCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCg       <  1:92554/150‑1 (MQ=255)
                                                                                                                                             tttaTGGCTTTATCTCTAACAAATTGTAATTAAACATTTACTTTTATTAAGGTATTTGTTGCACCCCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGTGCTATGTCTCTTCATTCTCCAGGTACAGCGTTTc   >  2:913858/4‑151 (MQ=255)
                                                                                                                                              tCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTc   <  2:813376/150‑1 (MQ=255)
                                                                                                                                              tCATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTc   <  2:898195/150‑1 (MQ=255)
                                                                                                                                               cATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTcg  <  2:287302/150‑1 (MQ=255)
                                                                                                                                               cATGGCTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTcg  <  2:337747/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAAAGTTAAGAAACCGAATATTGGGTTTAGTCTTGTTTCATAATTGTTGCAATGAAACGCGGTGAAACATTGCCTGAAACGTTAACTGAAACGCATATTTGCGGATTAGTTCATGACTTTATCTCTAACAAATTGAAATTAAACATTTAATTTTATTAAGGCAATTGTGGCACACCCCTTGCTTTGTCTTTATCAACGCAAATAACAAGTTGATAACAAAGGATGGGCTATGTCTCTACACTCTCCAGGTAAAGCGTTTCG  >  NC_000913/348421‑348713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: