Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,478,998	T→G	H537P (CAT→CCT)	kefB ←	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,478,998	0	T	G	100.0%	217.5 / NA	58	H537P (CAT→CCT)	kefB	potassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/27);  total (31/27)

CACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCT  >  NC_000913/3478852‑3479148                                                                                                                                                   |                                                                                                                                                       cACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGaggca                                                                                                                                                     <  1:630751/150‑1 (MQ=255)   cGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGaggcatgc                                                                                                                                                  >  1:573568/1‑151 (MQ=255)         aaaaTCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCg                                                                                                                                            <  1:690658/151‑1 (MQ=255)          aaaTCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCg                                                                                                                                            >  2:453118/1‑150 (MQ=255)          aaaTCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGt                                                                                                                                           >  1:718404/1‑151 (MQ=255)                ctTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGAcc                                                                                                                                       >  1:619677/1‑149 (MQ=255)                ctTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGAcc                                                                                                                                       >  1:410944/1‑149 (MQ=255)                 ttCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGc                                                                                                                                    >  2:702426/1‑151 (MQ=255)                  tCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCa                                                                                                                                      <  1:824564/148‑1 (MQ=255)                  tCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCg                                                                                                                                   >  2:847056/1‑151 (MQ=255)                        tCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTg                                                                                                                              >  1:273407/1‑150 (MQ=255)                           cgTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGcc                                                                                                                          >  1:514028/1‑151 (MQ=255)                            gTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGccc                                                                                                                         >  2:49262/1‑151 (MQ=255)                            gTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGccc                                                                                                                         >  2:851313/1‑151 (MQ=255)                              cGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCa                                                                                                                       <  2:787965/151‑1 (MQ=255)                                ggCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGc                                                                                                                     <  1:669193/151‑1 (MQ=255)                                ggCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGc                                                                                                                     >  1:171797/1‑151 (MQ=255)                                ggCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGc                                                                                                                     <  1:456066/151‑1 (MQ=255)                                      ccTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAAc                                                                                                               >  1:223637/1‑151 (MQ=255)                                         ggCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACg                                                                                                              <  1:476514/149‑1 (MQ=255)                                          gcgcGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCAc                                                                                                           >  1:653287/1‑151 (MQ=255)                                             cgAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTgg                                                                                                        <  2:194099/151‑1 (MQ=255)                                              gagaAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGga                                                                                                       <  1:614168/151‑1 (MQ=255)                                                      tGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGtt                                                                                                <  2:373704/150‑1 (MQ=255)                                                        tACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTc                                                                                              >  2:289914/1‑150 (MQ=255)                                                          cGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACg                                                                                           >  2:890337/1‑151 (MQ=255)                                                           ggTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACgg                                                                                          <  2:491794/151‑1 (MQ=255)                                                            gTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACggg                                                                                         >  1:427121/1‑151 (MQ=255)                                                              aTCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGaa                                                                                       <  1:634710/151‑1 (MQ=255)                                                                cGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGaaaa                                                                                     <  1:635225/151‑1 (MQ=255)                                                                cGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGaaaa                                                                                     <  2:671712/151‑1 (MQ=255)                                                                  gcatgcatTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACt                                                                                   >  1:44027/1‑151 (MQ=255)                                                                    atgcatTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGc                                                                                 >  1:872991/1‑151 (MQ=255)                                                                      gcatTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCg                                                                                <  1:360554/150‑1 (MQ=255)                                                                       catTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTc                                                                              >  2:903509/1‑151 (MQ=255)                                                                        atTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCa                                                                             <  1:896433/151‑1 (MQ=255)                                                                               tGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCcctgc                                                                      >  2:613483/1‑151 (MQ=255)                                                                                     ctcGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGt                                                                  <  1:435334/149‑1 (MQ=255)                                                                                           gcaTCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTc                                                          <  1:275694/151‑1 (MQ=255)                                                                                                  cATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCt                                                   >  1:571674/1‑151 (MQ=255)                                                                                                   atatCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCtt                                                  >  2:458812/1‑151 (MQ=255)                                                                                                    tatCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTc                                                 >  2:171776/1‑151 (MQ=255)                                                                                                      tCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAATTCATGCGCTTcc                                                >  1:43159/1‑150 (MQ=255)                                                                                                       ccAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCcac                                              <  1:66877/151‑1 (MQ=255)                                                                                                        cAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCcac                                              >  2:309931/1‑150 (MQ=255)                                                                                                          ggcggcGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACg                                           <  1:251715/151‑1 (MQ=255)                                                                                                            cggcgAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTc                                         >  1:525819/1‑151 (MQ=255)                                                                                                            cggcgAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTc                                         <  1:833600/151‑1 (MQ=255)                                                                                                              gcgAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCcg                                       <  2:527691/151‑1 (MQ=255)                                                                                                              gcgAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCcg                                       >  2:616287/1‑151 (MQ=255)                                                                                                               cgAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTcc                                        >  2:77901/1‑149 (MQ=255)                                                                                                                gAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCcgcg                                     >  1:483393/1‑151 (MQ=255)                                                                                                                    aTGCAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCt                                 <  1:110905/151‑1 (MQ=255)                                                                                                                       cAGTTGCGCTCGCTGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTcg                               >  2:896993/1‑150 (MQ=255)                                                                                                                                 cgctGTGCCTGATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAatat                    <  1:209349/151‑1 (MQ=255)                                                                                                                                           gATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGc            <  2:515744/149‑1 (MQ=255)                                                                                                                                           gATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGc            <  2:791498/149‑1 (MQ=255)                                                                                                                                           gATGCGGAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCgg          <  1:637592/151‑1 (MQ=255)                                                                                                                                                ggAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGt     <  2:538456/151‑1 (MQ=255)                                                                                                                                                ggAGGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGt     >  2:126270/1‑151 (MQ=255)                                                                                                                                                   tgcatgcCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCt  >  2:529806/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                       CACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCT  >  NC_000913/3478852‑3479148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑