Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,493,473 T→G A302A (GCT→GCG tsgA → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,493,4730TG98.3% 208.5 / ‑6.8 59A302A (GCT→GCGtsgAputative transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (28/30);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCTTTGTGGTTACCGGCC  >  NC_000913/3493326‑3493615
                                                                                                                                                   |                                                                                                                                              
aTACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTg                                                                                                                                             >  2:494098/1‑151 (MQ=255)
    aTGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGct                                                                                                                                         <  2:806652/151‑1 (MQ=255)
     tGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGctt                                                                                                                                        >  2:255699/1‑151 (MQ=255)
      ggTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGcttc                                                                                                                                       <  1:49143/151‑1 (MQ=255)
      ggTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGctt                                                                                                                                        <  2:626749/150‑1 (MQ=255)
          ggCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCttcttc                                                                                                                                    >  2:625708/1‑150 (MQ=255)
          ggCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCttcttc                                                                                                                                    >  2:785886/1‑150 (MQ=255)
           gCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTtctc                                                                                                                                  >  2:904171/1‑151 (MQ=255)
            cATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTcc                                                                                                                                 >  1:262397/1‑151 (MQ=255)
                 gggCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAgcgc                                                                                                                            <  1:195953/151‑1 (MQ=255)
                  ggCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGcgcg                                                                                                                           <  2:668674/151‑1 (MQ=255)
                     gTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGGCAATTTTCGCTCTGGGCTTCTTCTCCAGcgcggt                                                                                                                         >  2:770176/1‑148 (MQ=255)
                      ttCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCt                                                                                                                       >  1:826083/1‑151 (MQ=255)
                             ttATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATaccac                                                                                                                >  1:105006/1‑151 (MQ=255)
                              tATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATaccac                                                                                                                <  1:368348/150‑1 (MQ=255)
                               aTTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATAccacca                                                                                                              <  1:880869/151‑1 (MQ=255)
                                 tcttcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACcat                                                                                                             <  1:572156/150‑1 (MQ=255)
                                    tcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACcatcat                                                                                                          <  1:806346/150‑1 (MQ=255)
                                    tcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACcatca                                                                                                           <  2:61096/149‑1 (MQ=255)
                                        ttcttTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAccc                                                                                                     >  1:505939/1‑151 (MQ=255)
                                         tcttTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAccc                                                                                                     >  1:197058/1‑150 (MQ=255)
                                            ttGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTgg                                                                                                  <  2:387277/150‑1 (MQ=255)
                                            ttGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTg                                                                                                   <  1:868044/149‑1 (MQ=255)
                                                 ttGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTcac                                                                                            >  2:836423/1‑151 (MQ=255)
                                                   gCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTcaca                                                                                           <  2:748668/150‑1 (MQ=255)
                                                    cAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAcagc                                                                                         <  1:128349/151‑1 (MQ=255)
                                                    cAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCCGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAcagc                                                                                         >  2:56532/1‑151 (MQ=255)
                                                      aCGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAcagcag                                                                                       <  2:857491/151‑1 (MQ=255)
                                                      aCGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAcagcag                                                                                       <  1:682907/151‑1 (MQ=255)
                                                               gACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGt                                                                               >  2:203822/1‑150 (MQ=255)
                                                                 ccGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTAcc                                                                            <  2:121955/151‑1 (MQ=255)
                                                                     aCTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTc                                                                         <  2:368418/150‑1 (MQ=255)
                                                                      ctggctggTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGc                                                                       >  2:415049/1‑151 (MQ=255)
                                                                       tggctggTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTc                                                                         <  1:359983/148‑1 (MQ=255)
                                                                          ctggTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCaa                                                                    >  1:539427/1‑150 (MQ=255)
                                                                                  gCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTa                                                           <  2:352985/151‑1 (MQ=255)
                                                                                   cTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTa                                                           >  1:434105/1‑150 (MQ=255)
                                                                                     gCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACt                                                        <  1:778575/151‑1 (MQ=255)
                                                                                       gATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACttt                                                      <  1:481823/151‑1 (MQ=255)
                                                                                          tCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTc                                                   >  1:674066/1‑151 (MQ=255)
                                                                                              aTGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGa                                               <  1:494111/151‑1 (MQ=255)
                                                                                                    gTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCg                                         >  2:139531/1‑151 (MQ=255)
                                                                                                      cTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAACACTGGTTAACTTTGTCCTGACCTGCgg                                        >  1:766068/1‑150 (MQ=255)
                                                                                                      cTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCgg                                        >  1:454386/1‑150 (MQ=255)
                                                                                                              ccGGAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCgg                               >  1:641962/1‑151 (MQ=255)
                                                                                                                ggAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTa                             <  2:795445/151‑1 (MQ=255)
                                                                                                                 gAACACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTAc                            <  2:607288/151‑1 (MQ=255)
                                                                                                                  aaCACCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTAc                            >  2:631638/1‑150 (MQ=255)
                                                                                                                   acacCAGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACt                           >  1:308790/1‑150 (MQ=255)
                                                                                                                        aGCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTg                     <  1:785904/151‑1 (MQ=255)
                                                                                                                         gCACATATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGa                    <  2:500404/151‑1 (MQ=255)
                                                                                                                           acaTATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGAcc                  <  1:904191/151‑1 (MQ=255)
                                                                                                                           acaTATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGAcc                  >  1:654579/1‑151 (MQ=255)
                                                                                                                            caTATGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCt                 >  2:370687/1‑151 (MQ=255)
                                                                                                                             atatGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCtt                >  1:372366/1‑151 (MQ=255)
                                                                                                                               atGGCGTGGTCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCTCCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCttt               <  1:378208/150‑1 (MQ=255)
                                                                                                                                        tCAATTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCTTTGTGGTTACCg     >  2:634829/1‑151 (MQ=255)
                                                                                                                                          aaTTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCTTTGTGGTTACCGGc   <  2:127571/151‑1 (MQ=255)
                                                                                                                                           aTTCTCGCGCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCTTTGTGGTTACCGGcc  >  2:600172/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
ATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCACAGCAGACCAAAGTACCGTCGCCAAAACTGGTTAACTTTGTCCTGACCTGCGGAACCATCGGTACTATGTTGACCTTTGTGGTTACCGGCC  >  NC_000913/3493326‑3493615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: