Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,565,832	A→C	G250G (GGT→GGG)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,832	0	A	C	100.0%	205.1 / NA	55	G250G (GGT→GGG)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/25);  total (30/25)

GGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTT  >  NC_000913/3565684‑3565979                                                                                                                                                     |                                                                                                                                                    ggcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTcccc                                                                                                                                                   <  2:257382/151‑1 (MQ=255)      aaaaTGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGt                                                                                                                                              >  1:84699/1‑151 (MQ=255)       aaaTGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGtt                                                                                                                                             <  2:161181/151‑1 (MQ=255)           gTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGaa                                                                                                                                          <  1:135055/150‑1 (MQ=255)                 aaTGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCg                                                                                                                                   <  2:116276/151‑1 (MQ=255)                  aTGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCg                                                                                                                                   >  1:573744/1‑150 (MQ=255)                  aTGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCg                                                                                                                                   >  2:202398/1‑150 (MQ=255)                    ggTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAg                                                                                                                                 <  2:651699/150‑1 (MQ=255)                        gAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTaa                                                                                                                            <  2:764287/151‑1 (MQ=255)                          ggAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAAtt                                                                                                                          >  2:768422/1‑151 (MQ=255)                            aaaCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTc                                                                                                                        <  2:603265/151‑1 (MQ=255)                                  ggAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACt                                                                                                                  <  2:37410/151‑1 (MQ=255)                                    aaGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTgg                                                                                                                >  1:671615/1‑151 (MQ=255)                                       tATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCtt                                                                                                             >  1:254100/1‑151 (MQ=255)                                        aTTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCtt                                                                                                             <  1:38312/150‑1 (MQ=255)                                          tCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTgc                                                                                                           <  2:910006/150‑1 (MQ=255)                                              gACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCAc                                                                                                       >  2:733481/1‑150 (MQ=255)                                               aCGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTc                                                                                                     <  1:42157/151‑1 (MQ=255)                                                     gcgcAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACaaa                                                                                               <  2:74511/151‑1 (MQ=255)                                                       gcAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACg                                                                                             >  1:832796/1‑151 (MQ=255)                                                         aGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCa                                                                                           <  1:176133/151‑1 (MQ=255)                                                             cACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCg                                                                                        <  1:550179/150‑1 (MQ=255)                                                                gCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGtt                                                                                    >  2:748459/1‑151 (MQ=255)                                                                gCCCGGAGGAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGTCCCCTCCACAAAAGCAGCgaggt                                                                                    >  2:113945/1‑147 (MQ=255)                                                                               aGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTc                                                                     <  2:201643/151‑1 (MQ=255)                                                                                  cATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTTCGCACTCCACAACCGCAGCGGGCTGGTCGCGTCCGTGGCGt                                                                   >  1:39880/1‑150 (MQ=255)                                                                                  cATCCGGATACAGTACACGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGt                                                                   >  2:898296/1‑150 (MQ=255)                                                                                     ccGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAcc                                                               <  2:254095/151‑1 (MQ=255)                                                                                        gATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAgg                                                            <  2:917187/151‑1 (MQ=255)                                                                                        gATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAgg                                                            >  2:706473/1‑151 (MQ=255)                                                                                        gATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAgg                                                            >  1:747258/1‑151 (MQ=255)                                                                                            cAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGAtt                                                        >  2:866191/1‑151 (MQ=255)                                                                                                aCGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATc                                                     >  2:269832/1‑150 (MQ=255)                                                                                                 cgcgAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTg                                                   <  1:255098/151‑1 (MQ=255)                                                                                                  gcgAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACCAACGCAGCGTGTTGGTAGCGACGGTGTCGTAACCAGGGATTATCTg                                                   >  2:746596/1‑150 (MQ=255)                                                                                                   cgAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGAt                                                 >  1:152647/1‑151 (MQ=255)                                                                                                         aCGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACCAACGCAGCGAGTTGGTCGCGTCGGCGTCGTAACCAGGGATTATCTGATCGTa                                             >  1:669041/1‑149 (MQ=255)                                                                                                         aCGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTa                                             >  1:559961/1‑149 (MQ=255)                                                                                                          cGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGc                                          <  2:547134/151‑1 (MQ=255)                                                                                                             tctcGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGa                                        >  1:355072/1‑150 (MQ=255)                                                                                                                   aGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTccc                                   >  1:759192/1‑149 (MQ=255)                                                                                                                    gTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTccc                                   >  1:627298/1‑148 (MQ=255)                                                                                                                    gTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGa                                <  2:781997/151‑1 (MQ=255)                                                                                                                      ggTTTTTATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGACGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAAt                              <  1:485308/151‑1 (MQ=255)                                                                                                                         ttttATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATATCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGGGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAAtctc                           >  2:543931/1‑151 (MQ=255)                                                                                                                          tttATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAAtctc                           <  1:471778/150‑1 (MQ=255)                                                                                                                            tATCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTc                        >  1:810210/1‑151 (MQ=255)                                                                                                                             aTCTTCCACAGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCg                       >  1:490956/1‑151 (MQ=255)                                                                                                                              tCTTCCACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCgg                      >  1:414680/1‑151 (MQ=255)                                                                                                                                   cACTGCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGttt                   >  1:821565/1‑149 (MQ=255)                                                                                                                                      ttccGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCCGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATc              >  1:783303/3‑151 (MQ=255)                                                                                                                                       gCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATcc             <  2:889898/151‑1 (MQ=255)                                                                                                                                       gCCGCGAAGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATc              <  2:832778/150‑1 (MQ=255)                                                                                                                                             aaGTAGTCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGcgcg       >  1:791506/1‑151 (MQ=255)                                                                                                                                              agtagtCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGt      <  1:22711/151‑1 (MQ=255)                                                                                                                                                  gtCCCCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGttttt  <  1:161068/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAATCTCTTCGGTTTCAATCCAGCGCGTTTTT  >  NC_000913/3565684‑3565979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑