Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	351,204	A→C	intergenic (+23/‑11)	prpC → / → prpD	2‑methylcitrate synthase/2‑methylcitrate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	351,204	0	A	C	100.0%	206.4 / NA	54	intergenic (+23/‑11)	prpC/prpD	2‑methylcitrate synthase/2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/32);  total (22/32)

CCGCGTCACCGGCTGGGCGGCGCACATTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGAAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTC  >  NC_000913/351058‑351349                                                                                                                                                   |                                                                                                                                                  ccGCGTCACCGGCTGGGCGGCGCACATTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACg                                                                                                                                               <  1:638195/151‑1 (MQ=255)              tGGGCGGCGCACATTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTc                                                                                                                                    >  1:267726/1‑149 (MQ=255)               gggCGGCGCACATTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCa                                                                                                                                   <  2:392742/149‑1 (MQ=255)                    gcgcACATTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCaa                                                                                                                             >  2:379421/1‑150 (MQ=255)                       cacaTTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATc                                                                                                                          >  2:848507/1‑150 (MQ=255)                               cGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACAt                                                                                                                  <  1:870232/150‑1 (MQ=255)                               cGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATc                                                                                                                 <  2:630013/151‑1 (MQ=255)                                 aacaacGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCg                                                                                                               <  1:249958/151‑1 (MQ=255)                                    aacGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGcc                                                                                                             >  1:271184/1‑150 (MQ=255)                                     acGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCg                                                                                                           <  2:174083/151‑1 (MQ=255)                                      cGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCgg                                                                                                          >  1:799324/1‑151 (MQ=255)                                       gTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGa                                                                                                         <  1:228714/151‑1 (MQ=255)                                          aGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAAtt                                                                                                      <  1:703241/151‑1 (MQ=255)                                              caacaaAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGAt                                                                                                  <  2:406752/151‑1 (MQ=255)                                               aacaaAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATc                                                                                                 >  2:161673/1‑151 (MQ=255)                                                acaaAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCg                                                                                                >  1:804146/1‑151 (MQ=255)                                                  aaaaTTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTg                                                                                              >  1:128644/1‑151 (MQ=255)                                                   aaaTTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGa                                                                                             <  2:130760/151‑1 (MQ=255)                                                    aaTTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGaa                                                                                            <  2:318398/151‑1 (MQ=255)                                                     aTTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGaa                                                                                            >  2:899577/1‑150 (MQ=255)                                                          ccGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGtt                                                                                      <  2:184602/151‑1 (MQ=255)                                                           cGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTg                                                                                     >  1:729572/1‑151 (MQ=255)                                                                 tCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATAtc                                                                                <  2:759134/150‑1 (MQ=255)                                                                  ccGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATAtcgt                                                                              <  2:640110/151‑1 (MQ=255)                                                                   cGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATAtcgtc                                                                             <  2:519932/151‑1 (MQ=255)                                                                   cGCCAATTATGTTGGACCAGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATAtcgtc                                                                             <  2:427675/151‑1 (MQ=255)                                                                     ccAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATAtcgtcg                                                                            >  2:594461/1‑150 (MQ=255)                                                                       aaTTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGAtt                                                                         <  1:203940/151‑1 (MQ=255)                                                                          tATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTAc                                                                       <  2:184165/150‑1 (MQ=255)                                                                                ggACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATg                                                                 >  1:623537/1‑150 (MQ=255)                                                                                ggACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGa                                                                <  1:549936/151‑1 (MQ=255)                                                                                     ggAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTAc                                                           >  1:479478/1‑151 (MQ=255)                                                                                             gCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAg                                                   <  1:664199/151‑1 (MQ=255)                                                                                                  tttGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGctct                                               >  2:448065/1‑150 (MQ=255)                                                                                                        gcgcTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTgg                                        <  2:1240/151‑1 (MQ=255)                                                                                                        gcgcTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTgg                                        <  2:570031/151‑1 (MQ=255)                                                                                                           cTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCt                                     >  2:830422/1‑151 (MQ=255)                                                                                                           cTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCt                                     >  2:414206/1‑151 (MQ=255)                                                                                                             ggATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCt                                     <  1:132442/149‑1 (MQ=255)                                                                                                                 aaGCGCCAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGac                                >  2:863973/1‑150 (MQ=255)                                                                                                                      ccAGTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGca                          <  2:159743/151‑1 (MQ=255)                                                                                                                         gTAAACCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACAt                       >  1:324770/1‑151 (MQ=255)                                                                                                                            aaCCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTAc                    <  2:689515/151‑1 (MQ=255)                                                                                                                            aaCCTCTACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTAc                    <  2:75689/151‑1 (MQ=255)                                                                                                                                 ctACGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTActgc                 <  1:375130/149‑1 (MQ=255)                                                                                                                                   aCGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTActgcctgc             <  1:31941/151‑1 (MQ=255)                                                                                                                                    cGAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCt            >  1:780427/1‑151 (MQ=255)                                                                                                                                     gAATAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTc           <  2:826208/151‑1 (MQ=255)                                                                                                                                       aTAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCg          >  1:591510/1‑150 (MQ=255)                                                                                                                                        tAACAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGac        >  2:565206/1‑151 (MQ=255)                                                                                                                                           cAATAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACg     <  1:83619/151‑1 (MQ=255)                                                                                                                                            aaTAAGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGc    >  1:577190/1‑151 (MQ=255)                                                                                                                                               aaGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTc  <  1:414215/150‑1 (MQ=255)                                                                                                                                               aaGGCAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTc  <  1:420017/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CCGCGTCACCGGCTGGGCGGCGCACATTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAAACCTCTACGAATAACAATAAGGAAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTCGATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTC  >  NC_000913/351058‑351349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑