Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,766,728	A→C	E131A (GAA→GCA)	yibA →	putative immunity protein for polymorphic toxin RhsA; HEAT‑domain protein; lethality reduction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,766,728	0	A	C	100.0%	251.1 / NA	65	E131A (GAA→GCA)	yibA	putative immunity protein for polymorphic toxin RhsA; HEAT‑domain protein; lethality reduction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/26);  total (39/26)

AAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGCCG  >  NC_000913/3766580‑3766874                                                                                                                                                     |                                                                                                                                                   aaaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCAc                                                                                                                                                  >  1:599013/1‑151 (MQ=255)      ttGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAAtct                                                                                                                                             >  2:253337/1‑151 (MQ=255)          aaaaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCaaa                                                                                                                                         <  1:607379/151‑1 (MQ=255)           aaaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAAt                                                                                                                                        >  2:101119/1‑151 (MQ=255)           aaaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAAt                                                                                                                                        <  1:397762/151‑1 (MQ=255)            aaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAAtt                                                                                                                                       >  1:155766/1‑151 (MQ=255)                 gCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGc                                                                                                                                  <  2:689911/151‑1 (MQ=255)                            gTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAAt                                                                                                                       <  1:636400/151‑1 (MQ=255)                               tttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCg                                                                                                                     <  2:504038/150‑1 (MQ=255)                               tttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCg                                                                                                                     <  2:374428/150‑1 (MQ=255)                                ttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGAc                                                                                                                   >  2:800123/1‑151 (MQ=255)                                  aaTATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACt                                                                                                                  <  2:322619/150‑1 (MQ=255)                                     atTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATg                                                                                                              >  1:798402/1‑151 (MQ=255)                                      ttttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGt                                                                                                             >  1:766883/1‑151 (MQ=255)                                        ttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTc                                                                                                            <  2:666989/150‑1 (MQ=255)                                         tGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCa                                                                                                           <  1:542527/150‑1 (MQ=255)                                           aaCAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGa                                                                                                         >  2:224926/1‑150 (MQ=255)                                           aaCAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGAc                                                                                                        >  1:214979/1‑151 (MQ=255)                                           aaCAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGAc                                                                                                        >  1:841681/1‑151 (MQ=255)                                                   ggCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTAc                                                                                                 >  1:291058/1‑150 (MQ=255)                                                    gCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTAc                                                                                                 >  1:791260/1‑149 (MQ=255)                                                    gCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAg                                                                                               <  1:628307/151‑1 (MQ=255)                                                      aTTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCa                                                                                             <  1:138162/151‑1 (MQ=255)                                                       ttGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCAt                                                                                            >  1:910177/1‑151 (MQ=255)                                                        tGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCAtt                                                                                           >  2:240200/1‑151 (MQ=255)                                                        tGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCAtt                                                                                           >  1:822703/1‑151 (MQ=255)                                                                aaGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTAttt                                                                                   <  2:485114/151‑1 (MQ=255)                                                                    gcgcTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGGAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGt                                                                               >  1:603366/1‑151 (MQ=255)                                                                      gcTTGCGTTCGAGCTACGGCAATCGAGTCCACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAACTAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGtt                                                                              >  2:776415/1‑150 (MQ=255)                                                                      gcTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGtt                                                                              >  2:565420/1‑150 (MQ=255)                                                                           cGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCACGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCaa                                                                         >  2:735708/1‑150 (MQ=255)                                                                             ttCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATg                                                                       >  2:70379/1‑150 (MQ=255)                                                                                    tACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGc                                                                >  1:361902/1‑150 (MQ=255)                                                                                      cGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGcaac                                                             >  1:817933/1‑151 (MQ=255)                                                                                         cAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAAt                                                          <  1:633042/151‑1 (MQ=255)                                                                                           aTCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTc                                                        <  1:884569/151‑1 (MQ=255)                                                                                                  cAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTAtt                                                 <  1:608689/151‑1 (MQ=255)                                                                                                   aaCGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTg                                                <  2:876960/151‑1 (MQ=255)                                                                                                     cGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGAt                                              >  2:82634/1‑151 (MQ=255)                                                                                                      ggCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGAtt                                             >  1:17150/1‑151 (MQ=255)                                                                                                       gCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGAtt                                             >  1:373602/1‑150 (MQ=255)                                                                                                       gCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGAtt                                             >  1:376533/1‑150 (MQ=255)                                                                                                        cTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTa                                            >  2:678194/1‑150 (MQ=255)                                                                                                          cAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATc                                         <  2:671630/151‑1 (MQ=255)                                                                                                            gCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTg                                       <  1:221122/151‑1 (MQ=255)                                                                                                              gATGCAAGAAAAACCCAATTCATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGtt                                     <  1:686284/151‑1 (MQ=255)                                                                                                               aTGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGtt                                     >  1:912284/1‑150 (MQ=255)                                                                                                               aTGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGtt                                     >  2:327890/1‑150 (MQ=255)                                                                                                                  cAAGAAAAACCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTaaa                                  >  2:872318/1‑150 (MQ=255)                                                                                                                        aaaCCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCa                           <  2:47905/151‑1 (MQ=255)                                                                                                                         aaCCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCaa                          <  1:225375/151‑1 (MQ=255)                                                                                                                          aCCCAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCaaa                         <  1:540811/151‑1 (MQ=255)                                                                                                                           cccAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAAt                        <  2:159273/151‑1 (MQ=255)                                                                                                                            ccAATTTATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATg                       >  1:82070/1‑151 (MQ=255)                                                                                                                                tttATTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATgg                      >  1:409520/1‑148 (MQ=255)                                                                                                                                   aTTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGt                >  2:575885/1‑151 (MQ=255)                                                                                                                                   aTTCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGt                >  2:123283/1‑151 (MQ=255)                                                                                                                                     tCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCaaa                         >  1:925268/1‑140 (MQ=255)                                                                                                                                     tCACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCaaa                         <  2:925248/140‑1 (MQ=255)                                                                                                                                      cACCTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCa              <  2:841663/150‑1 (MQ=255)                                                                                                                                        ccTAAAATAGTAGCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGa            >  2:705858/1‑150 (MQ=255)                                                                                                                                               tagtagCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGc    >  2:271477/1‑151 (MQ=255)                                                                                                                                               tagtagCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGc    >  2:658072/1‑151 (MQ=255)                                                                                                                                                agtagCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGcc   >  2:847444/1‑151 (MQ=255)                                                                                                                                                 gtagCACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGCCg  <  1:727251/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTGATAAATCGACTAATGTCAGACGTGCTACAGCATTTGCTATTTCTGTTATCAATGATAAAGCAACAATTCCACTATTGATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGCCG  >  NC_000913/3766580‑3766874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑