Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,780,450 | A→C | S208S (TCA→TCC) | lldD → | L‑lactate dehydrogenase, FMN‑linked |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,780,450 | 0 | A | C | 98.4% | 224.1 / NA | 62 | S208S (TCA→TCC) | lldD | L‑lactate dehydrogenase, FMN‑linked |
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (31/30); total (31/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CACCGGGCGCACGCTACCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCAGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGATCCGGA > NC_000913/3780305‑3780596 | cacCGGGCGCACGCTACCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCtt > 2:268437/1‑150 (MQ=255) gggCGCACGCTACCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTAtctc < 1:135777/151‑1 (MQ=255) aCGCTACCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCaa > 2:634104/1‑150 (MQ=255) gCTACCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAacc < 2:406843/151‑1 (MQ=255) tACCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAaccg > 1:742956/1‑150 (MQ=255) aCCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAaccg < 2:536965/149‑1 (MQ=255) gTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCgg < 2:555061/151‑1 (MQ=255) tGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCgg > 1:584715/1‑150 (MQ=255) aTTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGa < 1:841459/151‑1 (MQ=255) aGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTAc < 1:661177/151‑1 (MQ=255) aTGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCg > 1:636981/1‑151 (MQ=255) aGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCggct < 2:676165/151‑1 (MQ=255) cgcgGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAAt > 1:530424/1‑151 (MQ=255) cgGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATa > 1:711538/1‑150 (MQ=255) cAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACtt < 1:434617/151‑1 (MQ=255) aaTGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTc < 2:633668/151‑1 (MQ=255) aTGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCg > 1:591438/1‑151 (MQ=255) tGCGCCGCTACTTGCAAGCGGGGAGACATCCGCAATGGGCGGGGGATGTGGGCCTGAACGGTCGTCAACATGATTTAGGCAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGACGATTACATCGGCTGGCTGGGGAATAACtt > 2:360067/1‑148 (MQ=255) ccgcTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGGGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTGATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATc > 2:369515/1‑149 (MQ=255) gcTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTc < 1:9434/151‑1 (MQ=255) gcTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTc < 2:334726/151‑1 (MQ=255) tACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTcc > 1:624343/1‑150 (MQ=255) aaGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATg < 2:712066/151‑1 (MQ=255) gTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAg < 2:683611/151‑1 (MQ=255) tGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGaa > 2:555130/1‑148 (MQ=255) acaTCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCtt < 1:104247/151‑1 (MQ=255) cagccGCAATGGGCGTGAGATGTGGGCCTGAGCGGTCGTCCACATGATTTAGGTAATATCTCAGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTACGATCCGTCCATCTCATGGTAAGACCTTg < 2:489888/148‑1 (MQ=255) tCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGa > 2:613955/1‑150 (MQ=255) tCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGa > 2:69863/1‑150 (MQ=255) ccGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAAt < 2:533871/151‑1 (MQ=255) ccGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAAt > 2:151006/1‑151 (MQ=255) gCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATgg > 1:696029/1‑151 (MQ=255) gggCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATcc > 2:857293/1‑150 (MQ=255) gggCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCcg > 1:481932/1‑151 (MQ=255) gCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCcgcg < 1:421757/151‑1 (MQ=255) gggATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGAttt < 2:501439/151‑1 (MQ=255) tgtgGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTgg > 2:687425/1‑151 (MQ=255) gtgGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTggg < 1:640685/151‑1 (MQ=255) gtgGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTgg < 1:308652/150‑1 (MQ=255) tgGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTggg > 2:676864/1‑150 (MQ=255) gggCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGa < 2:610640/150‑1 (MQ=255) gggCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGAt < 1:309822/151‑1 (MQ=255) ggCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATg < 1:351871/151‑1 (MQ=255) tGAACGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGccc > 2:106196/1‑151 (MQ=255) aaCGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCg > 2:724247/1‑150 (MQ=255) aaCGGTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGa < 2:163416/151‑1 (MQ=255) ggTCGTCCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATgg > 1:762633/1‑151 (MQ=255) gtcgtcCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGt > 1:539078/1‑151 (MQ=255) gtcgtcCACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGt < 2:412640/151‑1 (MQ=255) ccACATGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATc > 2:155893/1‑150 (MQ=255) caTGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAg < 2:200031/151‑1 (MQ=255) aTGATTTAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAgg < 1:561566/151‑1 (MQ=255) ttAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATc < 1:395101/150‑1 (MQ=255) ttAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATc < 2:665094/150‑1 (MQ=255) tAGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCt > 2:437362/1‑151 (MQ=255) aGGTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCt < 2:614318/150‑1 (MQ=255) gTAATATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCg > 2:276577/1‑150 (MQ=255) aaTATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGAt < 1:501365/150‑1 (MQ=255) aaTATCTCCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGATc > 2:174323/1‑151 (MQ=255) tctcCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGATCCgg > 1:874774/1‑150 (MQ=255) ctcCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGATCCGGa > 2:395510/1‑150 (MQ=255) ctcCGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGATCCGGa > 1:13356/1‑150 (MQ=255) | CACCGGGCGCACGCTACCGTGATGCGCATTCAGGTATGAGCGGCCCGAACGCGGCAATGCGCCGCTACTTGCAAGCGGTGACACATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCAGCTTATCTCGGCAAACCGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAATGGATCCGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGATCCGGA > NC_000913/3780305‑3780596 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |