Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,806,922	A→C	F49C (TTT→TGT)	waaG ←	UDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,806,922	0	A	C	100.0%	194.3 / NA	51	F49C (TTT→TGT)	waaG	UDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/30);  total (21/30)

GGCAAAATAAACATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATAAACAAAACGCCACGATC  >  NC_000913/3806774‑3807065                                                                                                                                                     |                                                                                                                                                ggCAAAATAAACATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACAt                                                                                                                                               <  1:339370/151‑1 (MQ=255) ggCAAAATAAACATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACAt                                                                                                                                               <  1:773410/151‑1 (MQ=255)  gCAAAATAAACATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATg                                                                                                                                              <  1:920498/151‑1 (MQ=255)      aaTAAACATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCttt                                                                                                                                          <  1:72600/151‑1 (MQ=255)         aaaCATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCgg                                                                                                                                       <  2:159642/151‑1 (MQ=255)          aaCATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCggg                                                                                                                                      <  2:235807/151‑1 (MQ=255)              tCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAAt                                                                                                                                  >  2:864140/1‑151 (MQ=255)                caggccaggcATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCg                                                                                                                                <  2:655629/151‑1 (MQ=255)                   gccaggcATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCt                                                                                                                             >  2:781775/1‑151 (MQ=255)                              tattaAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACtg                                                                                                                  >  1:30411/1‑151 (MQ=255)                               attaAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACtgt                                                                                                                 >  1:6409/1‑151 (MQ=255)                                      ccAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatata                                                                                                          <  1:554879/151‑1 (MQ=255)                                      ccAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGtatata                                                                                                          <  1:553853/151‑1 (MQ=255)                                           aacGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCg                                                                                                      >  1:306167/1‑150 (MQ=255)                                           aacGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGa                                                                                                     <  2:602585/151‑1 (MQ=255)                                           aacGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCACCTTCCCACGACTGTGTATATACCCg                                                                                                      >  2:8469/1‑150 (MQ=255)                                              gcgATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAAc                                                                                                   >  1:649590/1‑150 (MQ=255)                                                 aTCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtgg                                                                                               >  2:528334/1‑151 (MQ=255)                                                 aTCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACAtgg                                                                                               >  2:741487/1‑151 (MQ=255)                                                          aTGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGt                                                                                      >  2:496007/1‑151 (MQ=255)                                                            gCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTg                                                                                     <  1:56274/150‑1 (MQ=255)                                                            gCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGc                                                                                    <  2:519995/151‑1 (MQ=255)                                                                 ttGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCa                                                                                <  2:855890/150‑1 (MQ=255)                                                                   gagaTGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACt                                                                             <  1:737823/151‑1 (MQ=255)                                                                             tGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCa                                                                    <  2:773571/150‑1 (MQ=255)                                                                              gTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAAt                                                                  <  1:267323/151‑1 (MQ=255)                                                                              gTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAAt                                                                  >  1:743651/1‑151 (MQ=255)                                                                                   cAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCa                                                             <  2:439927/151‑1 (MQ=255)                                                                                    aGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCAt                                                            <  2:381130/151‑1 (MQ=255)                                                                                        ataataTTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATaa                                                          >  1:867609/1‑149 (MQ=255)                                                                                        ataataTTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATaa                                                          >  1:625047/1‑149 (MQ=255)                                                                                           atatTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGCTGCAATGCGCATAAAGTc                                                      >  2:843184/1‑150 (MQ=255)                                                                                               tCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGtt                                                 >  2:201904/1‑151 (MQ=255)                                                                                                    aTTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTGTATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGAATAAAGTCACGTTGAAg                                             <  2:369666/150‑1 (MQ=255)                                                                                                    aTTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGc                                            <  2:16743/151‑1 (MQ=255)                                                                                                          tCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCccacc                                       >  1:791756/1‑150 (MQ=255)                                                                                                           ccATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCccacca                                      <  2:671218/150‑1 (MQ=255)                                                                                                             aTGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAAt                                   <  2:859498/151‑1 (MQ=255)                                                                                                             aTGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAAt                                   <  1:502335/151‑1 (MQ=255)                                                                                                               ggttggtATGGGACTTAACTGGCACCTGAATAAGCTCACATGCGTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGTACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCGACCAAATg                                  <  2:613978/150‑1 (MQ=255)                                                                                                                   ggtATGGGACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGaaa                              <  2:900536/150‑1 (MQ=255)                                                                                                                         ggACTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATAttt                        >  1:255800/1‑150 (MQ=255)                                                                                                                           aCTTAACTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTtata                     <  2:743633/151‑1 (MQ=255)                                                                                                                               aaCTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATaa                  <  1:747386/150‑1 (MQ=255)                                                                                                                               aaCTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATaa                  <  1:738558/150‑1 (MQ=255)                                                                                                                               aaCTGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATaa                  >  1:677604/1‑150 (MQ=255)                                                                                                                                  tGGCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATAAACa               >  2:523418/1‑150 (MQ=255)                                                                                                                                   ggCACCTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATAAACa               <  2:306159/149‑1 (MQ=255)                                                                                                                                   ggCACCTGAATAAGCTCACATGCTTTCGGGCAATCACCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATAAACa               <  1:8469/149‑1 (MQ=255)                                                                                                                                        cTGAATAAGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATAAACAAAACGcc        >  2:617938/1‑151 (MQ=255)                                                                                                                                               aGCTCACATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATAAACAAAACGCCACGATc  >  1:99876/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                GGCAAAATAAACATCCAGGCCAGGCATCTTATTAAACCCAACAACGCGATCTGCGGGATGCTCTTTGAGATGATTTTGTACCCAGGCATAATATTCTGCATTGCGTCCATGGTTGGTATGGGACTTAACTGGCACCTGAATAAGCTCAAATGCTTTCGGGCAATCGCCTTCCCACGACTGTGTATATACCCGAACATGGTGGCCCCGTGCGGCAACTGTTGATGCAATGCGCATAAAGTCACGTTGAAGCCCACCAAATGGAAAATATTTATATAAACAAAACGCCACGATC  >  NC_000913/3806774‑3807065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑