Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,833,087	T→G	Y484S (TAC→TCC)	yicI ←	putative alpha‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,833,087	0	T	G	100.0%	192.4 / NA	52	Y484S (TAC→TCC)	yicI	putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/27);  total (25/27)

AAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGTAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGTTATGCATTT  >  NC_000913/3832943‑3833234                                                                                                                                                 |                                                                                                                                                    aaaccaaacGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAAc                                                                                                                                                  <  2:48875/148‑1 (MQ=255)       aacGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCAc                                                                                                                                         >  1:489898/1‑151 (MQ=255)         cgcacgccAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTAAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCGGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCcccc                                                                                                                                        <  2:654877/144‑1 (MQ=255)                    gTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCgg                                                                                                                            >  2:831812/1‑151 (MQ=255)                      ttGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCggg                                                                                                                           >  2:198530/1‑150 (MQ=255)                          aaaCGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAAtt                                                                                                                       <  1:752565/150‑1 (MQ=255)                             cGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCATGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCt                                                                                                                    <  1:757126/150‑1 (MQ=255)                              gTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTgc                                                                                                                  <  1:364214/151‑1 (MQ=255)                               tGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTgcg                                                                                                                 >  1:406828/1‑151 (MQ=255)                                  gcCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTgcgc                                                                                                                <  2:378925/149‑1 (MQ=255)                                     ggggCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTGTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCTGGAATTTCTGCGCGCCGa                                                                                                           <  2:532962/148‑1 (MQ=255)                                          ggTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGa                                                                                                       <  1:809619/150‑1 (MQ=255)                                               tttCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCg                                                                                                  >  2:541268/1‑150 (MQ=255)                                                   aaaGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCg                                                                                              <  1:220481/150‑1 (MQ=255)                                                       ccgccgATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCa                                                                                          >  1:563011/1‑150 (MQ=255)                                                         gccgATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAAc                                                                                       >  2:323018/1‑151 (MQ=255)                                                            gATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAg                                                                                    <  2:300811/151‑1 (MQ=255)                                                             atatCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGa                                                                                   <  1:94863/151‑1 (MQ=255)                                                              tatCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGAc                                                                                  >  2:587615/1‑151 (MQ=255)                                                               atCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGAc                                                                                  <  2:646927/150‑1 (MQ=255)                                                               atCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACa                                                                                 <  1:259309/151‑1 (MQ=255)                                                                   tGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGctt                                                                             >  2:734221/1‑151 (MQ=255)                                                                            aaGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCAc                                                                    >  2:260433/1‑151 (MQ=255)                                                                                        gAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTc                                                          <  1:883626/149‑1 (MQ=255)                                                                                        gAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCt                                                        >  1:485988/1‑151 (MQ=255)                                                                                           aGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGa                                                     <  2:851291/151‑1 (MQ=255)                                                                                            ggCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAg                                                    <  2:389662/151‑1 (MQ=255)                                                                                                aaTAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACg                                                >  1:325674/1‑151 (MQ=255)                                                                                                 aTAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACAGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGt                                               >  2:785393/1‑151 (MQ=255)                                                                                                   aGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGtt                                              <  2:198240/150‑1 (MQ=255)                                                                                                        aaCCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCacac                                        >  2:446561/1‑151 (MQ=255)                                                                                                           caccGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACAcc                                       >  2:564041/1‑149 (MQ=255)                                                                                                            accGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGt                                    >  2:290009/1‑151 (MQ=255)                                                                                                             ccGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGtt                                   <  2:193476/151‑1 (MQ=255)                                                                                                                cgcAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGt                                <  1:120262/151‑1 (MQ=255)                                                                                                                 gcAGGCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGtt                               >  2:845064/1‑151 (MQ=255)                                                                                                                    ggCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAATGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTa                            <  2:223432/151‑1 (MQ=255)                                                                                                                     gCTTTCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAg                           <  1:34692/151‑1 (MQ=255)                                                                                                                       tttCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGt                             >  1:496626/1‑147 (MQ=255)                                                                                                                        ttCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGa                          <  2:15726/149‑1 (MQ=255)                                                                                                                         tCCGCCATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGt                       >  1:614473/1‑151 (MQ=255)                                                                                                                             ccATTGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGc                   >  1:334139/1‑151 (MQ=255)                                                                                                                                 tGATTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATa                <  1:375972/150‑1 (MQ=255)                                                                                                                                   aTTCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTGCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATg             <  2:118273/151‑1 (MQ=255)                                                                                                                                    ttCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATgg            >  2:197250/1‑151 (MQ=255)                                                                                                                                     tCGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGt           >  2:703973/1‑151 (MQ=255)                                                                                                                                      cGTAGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGtt          >  1:728406/1‑151 (MQ=255)                                                                                                                                         aGTTAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGTTATg       <  2:118513/151‑1 (MQ=255)                                                                                                                                            tAGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGTTATGCa     <  1:66191/150‑1 (MQ=255)                                                                                                                                             aGCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGTTATGCAtt   <  1:222053/151‑1 (MQ=255)                                                                                                                                              gCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGTTATGCAttt  >  2:541105/1‑151 (MQ=255)                                                                                                                                              gCGGAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGTTATGCAttt  >  1:502563/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    AAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGTAACAATCGCCACCCCAGTGTACCGGGAATTTCTGCGCACCGACGGAGGCCGAGCGGGCAAACAAGACAGCTTCTTCCTCACCAACGGTGTCCTTGAGCACGTTCCACACCAGTTCGTTGTAGATGTACGCATAATGGTTATGCATTT  >  NC_000913/3832943‑3833234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑