Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	368,831	A→C	D74A (GAT→GCT)	mhpA →	3‑(3‑hydroxyphenyl)propionate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	368,831	0	A	C	100.0%	170.5 / NA	45	D74A (GAT→GCT)	mhpA	3‑(3‑hydroxyphenyl)propionate hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/23);  total (22/23)

GTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTG  >  NC_000913/368686‑368976                                                                                                                                                  |                                                                                                                                                  gTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGctgatg                                                                                                                                              <  2:336904/151‑1 (MQ=255)         gatgatGGCGAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCg                                                                                                                                      >  1:697829/1‑151 (MQ=255)          atgatgGCGAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGc                                                                                                                                     <  1:749200/151‑1 (MQ=255)                gCGAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTa                                                                                                                               <  2:65665/151‑1 (MQ=255)                       aTCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTg                                                                                                                        >  1:461396/1‑151 (MQ=255)                        tctcGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTgg                                                                                                                       >  1:66221/1‑151 (MQ=255)                          tcGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGc                                                                                                                      >  1:864827/1‑150 (MQ=255)                          tcGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGc                                                                                                                      >  1:469037/1‑150 (MQ=255)                           cGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCa                                                                                                                     <  2:641765/150‑1 (MQ=255)                           cGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCAc                                                                                                                    <  2:420284/151‑1 (MQ=255)                                 gATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATg                                                                                                              >  2:172895/1‑151 (MQ=255)                                 gATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATg                                                                                                              >  1:436945/1‑151 (MQ=255)                                  aTGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGc                                                                                                             <  1:701584/151‑1 (MQ=255)                                      gCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGttt                                                                                                         >  2:447699/1‑151 (MQ=255)                                        aTTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGtttt                                                                                                        >  2:476351/1‑150 (MQ=255)                                        aTTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGtttt                                                                                                        <  1:665323/150‑1 (MQ=255)                                           gACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTtctc                                                                                                     >  2:150629/1‑150 (MQ=255)                                                gCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTAGCACGCGATGCGTTTTCTCAcccc                                                                                                <  1:240456/150‑1 (MQ=255)                                                   ggtggtggAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGaaa                                                                                            <  2:171393/151‑1 (MQ=255)                                                     tggtggAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAgg                                                                                          >  2:917674/1‑151 (MQ=255)                                                       gtggAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGcc                                                                                        >  1:573311/1‑151 (MQ=255)                                                             aaaCTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGtt                                                                                  <  1:79715/151‑1 (MQ=255)                                                                  ccaTAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCt                                                                             <  1:475271/149‑1 (MQ=255)                                                                      aaGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGata                                                                         <  1:586224/151‑1 (MQ=255)                                                                         ttGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTc                                                                      >  2:471984/1‑151 (MQ=255)                                                                              cGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGcc                                                                  >  2:845526/1‑150 (MQ=255)                                                                                 cTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATg                                                              >  2:77205/1‑151 (MQ=255)                                                                                  tACCCGCGTGCGATTGGTATTGATGCCGAGGAGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATg                                                              >  2:741999/1‑150 (MQ=255)                                                                                             gATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAAttt                                                  >  1:701529/1‑151 (MQ=255)                                                                                                  gTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTtggctg                                             <  2:732529/151‑1 (MQ=255)                                                                                                    aTTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTtggctggc                                           <  1:635435/151‑1 (MQ=255)                                                                                                     ttGCTGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGcc                                          >  2:232421/1‑151 (MQ=255)                                                                                                      tgatgaCGAGGCGCTGCTCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCGGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCGCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAACCAATGACCGATGAATTTGGCTGGcc                                          <  2:162486/150‑1 (MQ=255)                                                                                                       gatgaCGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCcgc                                        <  1:723273/151‑1 (MQ=255)                                                                                                                 cgcTGCGCACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGc                              >  2:456283/1‑151 (MQ=255)                                                                                                                     gcgcACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCttt                          <  2:168977/151‑1 (MQ=255)                                                                                                                     gcgcACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCtt                           <  2:381179/150‑1 (MQ=255)                                                                                                                      cacACCATGCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCttt                          >  1:587387/3‑150 (MQ=255)                                                                                                                              gCAGTCGGTCGGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCg                 >  2:786128/1‑151 (MQ=255)                                                                                                                                 gtcggtcgGCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAg              <  1:655951/151‑1 (MQ=255)                                                                                                                                    ggtcggCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTc           <  2:123939/151‑1 (MQ=255)                                                                                                                                     gtcggCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCg          <  2:214214/151‑1 (MQ=255)                                                                                                                                       cggCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGAt        <  1:636806/151‑1 (MQ=255)                                                                                                                                       cggCCTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGAt        <  2:813868/151‑1 (MQ=255)                                                                                                                                           cTGGTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCgg    >  2:629923/1‑151 (MQ=255)                                                                                                                                             ggTCGCTGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTg  <  2:135466/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GTTGGGCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACCCGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTG  >  NC_000913/368686‑368976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑