Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,968,363	A→C	N150H (AAT→CAT)	wecA →	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,968,363	0	A	C	100.0%	236.4 / NA	62	N150H (AAT→CAT)	wecA	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/30);  total (32/30)

GCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTG  >  NC_000913/3968216‑3968506                                                                                                                                                    |                                                                                                                                                gCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATg                                                                                                                                              >  1:468281/1‑151 (MQ=255)       aTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTc                                                                                                                                         >  1:335630/1‑150 (MQ=255)           aGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACAt                                                                                                                                    <  2:813192/151‑1 (MQ=255)             gCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATgg                                                                                                                                  >  1:478229/1‑151 (MQ=255)              ccGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGt                                                                                                                                 >  1:694495/1‑151 (MQ=255)                gCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTg                                                                                                                               >  2:170570/1‑151 (MQ=255)                 cTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTg                                                                                                                               >  2:820774/1‑150 (MQ=255)                  tGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGAt                                                                                                                             >  1:749626/1‑151 (MQ=255)                      ggCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGc                                                                                                                          <  2:156138/150‑1 (MQ=255)                          ttGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTg                                                                                                                      <  1:224652/150‑1 (MQ=255)                            gTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGAt                                                                                                                    <  2:560565/150‑1 (MQ=255)                                     gtgtTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTgg                                                                                                          <  2:365262/151‑1 (MQ=255)                                        ttCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCg                                                                                                       >  1:94714/1‑151 (MQ=255)                                          cGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCggg                                                                                                     <  1:440523/151‑1 (MQ=255)                                            gCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGtt                                                                                                   >  2:789700/1‑151 (MQ=255)                                                  tttATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTg                                                                                             >  2:373222/1‑151 (MQ=255)                                                   ttATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCt                                                                                              <  1:880319/149‑1 (MQ=255)                                                   ttATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCt                                                                                              <  1:256365/149‑1 (MQ=255)                                                   ttATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGc                                                                                            >  2:132060/1‑151 (MQ=255)                                                    tATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCg                                                                                           >  2:640107/1‑151 (MQ=255)                                                         cAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtct                                                                                        <  1:886289/149‑1 (MQ=255)                                                         cAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtc                                                                                         <  2:656539/148‑1 (MQ=255)                                                           gTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGtt                                                                                    >  1:840555/1‑151 (MQ=255)                                                            tAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCTACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGttt                                                                                   >  2:675246/1‑151 (MQ=255)                                                             aGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGttt                                                                                   <  1:155026/150‑1 (MQ=255)                                                             aGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGttt                                                                                   <  2:422371/150‑1 (MQ=255)                                                             aGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGttt                                                                                   <  1:654378/150‑1 (MQ=255)                                                             aGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTg                                                                                  >  1:4683/1‑151 (MQ=255)                                                              gCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgc                                                                                 >  1:222114/1‑151 (MQ=255)                                                               ccTGGGATATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGttt                                                                                   >  2:520775/1‑148 (MQ=255)                                                                 tGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgcagc                                                                              >  2:450983/1‑151 (MQ=255)                                                                      tataTCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATc                                                                          <  2:516045/150‑1 (MQ=255)                                                                        tatCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGt                                                                       >  1:432843/1‑151 (MQ=255)                                                                            tttAGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGAAGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCggaa                                                                      >  2:707166/1‑146 (MQ=255)                                                                             ttGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGa                                                                   <  2:4011/150‑1 (MQ=255)                                                                                gCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAtttt                                                               >  1:856193/1‑151 (MQ=255)                                                                                 cTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTtg                                                              >  2:721345/1‑151 (MQ=255)                                                                                   ccTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTtgtg                                                            <  2:694478/151‑1 (MQ=255)                                                                                     tGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGt                                                          >  1:762144/1‑151 (MQ=255)                                                                                      gggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTgg                                                           <  2:834670/149‑1 (MQ=255)                                                                                      gggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTgg                                                           <  1:231523/149‑1 (MQ=255)                                                                                      gggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTgg                                                           <  2:627734/149‑1 (MQ=255)                                                                                      gggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGtt                                                         >  1:320380/1‑151 (MQ=255)                                                                                        gagaTGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCg                                                       >  1:908648/1‑151 (MQ=255)                                                                                         agaTGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGa                                                      <  2:918704/151‑1 (MQ=255)                                                                                           aTGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACg                                                    <  1:132061/151‑1 (MQ=255)                                                                                           aTGGTGCTCGGACCGCTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGAc                                                     >  2:787049/1‑150 (MQ=255)                                                                                              gTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGc                                                 >  2:226997/1‑151 (MQ=255)                                                                                                gCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCaa                                               <  1:332455/151‑1 (MQ=255)                                                                                                  tCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAAc                                             >  1:807952/1‑151 (MQ=255)                                                                                                   cGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAAcc                                            <  2:330726/151‑1 (MQ=255)                                                                                                    ggACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCa                                           <  2:243945/151‑1 (MQ=255)                                                                                                              gTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAAt                                 <  2:100045/151‑1 (MQ=255)                                                                                                                 aCTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTg                              >  2:641178/1‑151 (MQ=255)                                                                                                                  cTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTgg                             >  2:117445/1‑151 (MQ=255)                                                                                                                        gACGCTATTTGCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCt                         <  1:541175/149‑1 (MQ=255)                                                                                                                                  gCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATcg             <  1:26178/151‑1 (MQ=255)                                                                                                                                  gCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATcg             >  2:237432/1‑151 (MQ=255)                                                                                                                                  gCCGTCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATcg             <  2:8241/151‑1 (MQ=255)                                                                                                                                         gggCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCgccgcc        <  2:676973/149‑1 (MQ=255)                                                                                                                                            cggcCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCt   <  2:429749/151‑1 (MQ=255)                                                                                                                                             ggcCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTg  >  1:447391/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                GCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTG  >  NC_000913/3968216‑3968506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑