Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,005,207 T→G S116A (TCC→GCC)  pldA → outer membrane phospholipase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,005,2070TG100.0% 216.7 / NA 59S116A (TCC→GCC) pldAouter membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/22);  total (37/22)

GATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCT  >  NC_000913/4005060‑4005353
                                                                                                                                                   |                                                                                                                                                  
gATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCt                                                                                                                                                 >  1:38846/1‑151 (MQ=255)
       aTAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGc                                                                                                                                           >  1:532073/1‑150 (MQ=255)
         aaaGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCaa                                                                                                                                         >  2:409046/1‑150 (MQ=255)
          aagaagCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACt                                                                                                                                       <  2:707576/151‑1 (MQ=255)
               gCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATGTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCa                                                                                                                                  <  2:606947/151‑1 (MQ=255)
                   ttGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCAGTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCaa                                                                                                                                 >  1:574526/1‑148 (MQ=255)
                      ccAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGa                                                                                                                           <  1:644485/151‑1 (MQ=255)
                       cAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGa                                                                                                                           >  1:382249/1‑150 (MQ=255)
                           tACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGt                                                                                                                      >  1:143761/1‑151 (MQ=255)
                                     cGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGtt                                                                                                            >  1:784688/1‑151 (MQ=255)
                                      ggAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGttt                                                                                                           <  2:118776/151‑1 (MQ=255)
                                       gAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGCGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTAACCGTTTc                                                                                                          <  2:230453/151‑1 (MQ=255)
                                       gAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTc                                                                                                          <  2:312837/151‑1 (MQ=255)
                                         aaaTGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGt                                                                                                        >  2:215244/1‑151 (MQ=255)
                                                     ggATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTAc                                                                                            <  2:82023/151‑1 (MQ=255)
                                                      gATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACg                                                                                           <  2:29229/151‑1 (MQ=255)
                                                       aTGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACg                                                                                           >  2:836309/1‑150 (MQ=255)
                                                       aTGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACg                                                                                           >  1:687211/1‑150 (MQ=255)
                                                         gAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGaa                                                                                         <  2:336740/150‑1 (MQ=255)
                                                         gAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAAc                                                                                        >  1:4644/1‑151 (MQ=255)
                                                         gAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAAc                                                                                        <  1:146238/151‑1 (MQ=255)
                                                           aGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAAcc                                                                                       >  2:35296/1‑150 (MQ=255)
                                                           aGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCg                                                                                      >  1:396983/1‑151 (MQ=255)
                                                           aGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCg                                                                                      <  1:714676/151‑1 (MQ=255)
                                                                 gTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTg                                                                                <  2:154029/151‑1 (MQ=255)
                                                                   ttCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGt                                                                               <  1:332306/150‑1 (MQ=255)
                                                                      aGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCt                                                                           >  2:701982/1‑151 (MQ=255)
                                                                       gTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTc                                                                          <  2:352809/151‑1 (MQ=255)
                                                                         tGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCgg                                                                        >  1:240074/1‑151 (MQ=255)
                                                                          gAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGt                                                                       >  2:156108/1‑151 (MQ=255)
                                                                          gAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGt                                                                       <  2:477571/151‑1 (MQ=255)
                                                                           aGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGtt                                                                      >  1:611163/1‑151 (MQ=255)
                                                                                 gCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCa                                                                <  2:354957/151‑1 (MQ=255)
                                                                                  cATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCAc                                                               <  2:123452/151‑1 (MQ=255)
                                                                                     ttCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCg                                                             >  1:104278/1‑150 (MQ=255)
                                                                                     ttCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCg                                                             >  1:649483/1‑150 (MQ=255)
                                                                                           tgtgGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTAcc                                                       >  2:48931/1‑150 (MQ=255)
                                                                                                cGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTg                                                 >  1:222675/1‑151 (MQ=255)
                                                                                                cGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTg                                                 >  1:604068/1‑151 (MQ=255)
                                                                                                     gATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGt                                            <  1:832297/151‑1 (MQ=255)
                                                                                                         ttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTgg                                         >  2:152950/1‑150 (MQ=255)
                                                                                                          tAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGAc                                       >  2:670984/1‑151 (MQ=255)
                                                                                                           aGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGAc                                       <  2:546295/150‑1 (MQ=255)
                                                                                                           aGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACg                                      <  1:393017/151‑1 (MQ=255)
                                                                                                                   aCTCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGcgcg                               >  2:271731/1‑150 (MQ=255)
                                                                                                                     tCGGTGTTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtg                            >  2:815638/1‑151 (MQ=255)
                                                                                                                         tgtTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                         >  2:720045/1‑150 (MQ=255)
                                                                                                                          gtTGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGgag                       <  1:827683/151‑1 (MQ=255)
                                                                                                                           ttGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                         >  1:499567/1‑148 (MQ=255)
                                                                                                                            tGGGTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                         >  2:434801/1‑147 (MQ=255)
                                                                                                                               gTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATggg                  >  1:5883/1‑151 (MQ=255)
                                                                                                                               gTGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGGTTTGCCACCGATTACCGTTTTGCAGGATGGACGCTGCGCGATGTGGAGATgcg                  >  2:879387/1‑149 (MQ=255)
                                                                                                                                tGCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgggg                 >  2:621378/1‑151 (MQ=255)
                                                                                                                                 gCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgggg                 <  2:93752/150‑1 (MQ=255)
                                                                                                                                 gCGTCTTATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGt                >  1:376465/1‑151 (MQ=255)
                                                                                                                                      ttATACGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGCTGGACGCTGCGCGATGTGGAGATGGGGTATAAc           >  1:836726/1‑151 (MQ=255)
                                                                                                                                        ataCGCAAAAAGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAAcc          >  2:657189/1‑150 (MQ=255)
                                                                                                                                              aaaaaGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGActc   >  2:478494/1‑151 (MQ=255)
                                                                                                                                                aaaGCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGActct  >  2:521007/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAAAATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCT  >  NC_000913/4005060‑4005353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: