Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,511	T→G	I238L (ATT→CTT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,511	0	T	G	100.0%	206.9 / NA	55	I238L (ATT→CTT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/27);  total (28/27)

GCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACG  >  NC_000913/4116365‑4116658                                                                                                                                                   |                                                                                                                                                    gCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGAcgc                                                                                                                                                 <  2:597431/151‑1 (MQ=255)   cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGt                                                                                                                                               >  2:220769/1‑151 (MQ=255)     ttttCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTgc                                                                                                                                             >  1:763575/1‑151 (MQ=255)           gATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCtt                                                                                                                                       <  2:545863/151‑1 (MQ=255)                 aCCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTgccgc                                                                                                                                 >  1:502392/1‑151 (MQ=255)                 aCCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTgccg                                                                                                                                  <  2:353365/150‑1 (MQ=255)                  ccGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTgccgcc                                                                                                                                >  1:486598/1‑151 (MQ=255)                   cGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCGCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTgccg                                                                                                                                  <  1:65163/148‑1 (MQ=255)                   cGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTgcc                                                                                                                                   >  2:65163/1‑147 (MQ=255)                   cGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCa                                                                                                                               <  1:769907/151‑1 (MQ=255)                      tttCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATg                                                                                                                            >  2:261011/1‑151 (MQ=255)                           cGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGt                                                                                                                       <  2:158190/151‑1 (MQ=255)                              cAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTg                                                                                                                    <  1:392020/151‑1 (MQ=255)                               aGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTg                                                                                                                    >  1:590270/1‑150 (MQ=255)                                     catcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat                                                                                                             >  1:367798/1‑151 (MQ=255)                                      atcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat                                                                                                             >  1:259186/1‑150 (MQ=255)                                        caGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACt                                                                                                          >  2:565146/1‑151 (MQ=255)                                              aaaGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaa                                                                                                    <  1:236595/151‑1 (MQ=255)                                              aaaGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGga                                                                                                     <  1:306443/150‑1 (MQ=255)                                                   agcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacg                                                                                               >  2:801388/1‑151 (MQ=255)                                                   agcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacg                                                                                               >  1:917732/1‑151 (MQ=255)                                                               aGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTgg                                                                                   >  1:241624/1‑151 (MQ=255)                                                                  tgtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGgc                                                                                  >  2:124262/1‑149 (MQ=255)                                                                   gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGgcagc                                                                               >  1:498727/1‑151 (MQ=255)                                                                         cGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAtctc                                                                          <  2:916519/150‑1 (MQ=255)                                                                               cccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCgg                                                                    >  2:860530/1‑150 (MQ=255)                                                                                ccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGaa                                                                  <  1:635264/151‑1 (MQ=255)                                                                                   tcttTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAat                                                                 <  1:110131/149‑1 (MQ=255)                                                                                       tCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCa                                                            <  1:85925/150‑1 (MQ=255)                                                                                                  tGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGc                                                 >  2:141085/1‑150 (MQ=255)                                                                                                   gACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAt                                               >  2:806759/1‑151 (MQ=255)                                                                                                     ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAttt                                             >  1:684342/1‑151 (MQ=255)                                                                                                       aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTg                                           >  1:720685/1‑151 (MQ=255)                                                                                                       aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTg                                           <  2:281229/151‑1 (MQ=255)                                                                                                         aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc                                         <  2:310397/151‑1 (MQ=255)                                                                                                            gcgcGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtcgtc                                      <  2:352923/151‑1 (MQ=255)                                                                                                              gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTccc                                    >  2:526457/1‑151 (MQ=255)                                                                                                              gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTccc                                    >  2:186190/1‑151 (MQ=255)                                                                                                                ggCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTccc                                    >  1:535297/1‑149 (MQ=255)                                                                                                                  ccTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                >  1:770619/1‑151 (MQ=255)                                                                                                                  ccTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                <  1:124263/151‑1 (MQ=255)                                                                                                                     gctgGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTcc                               <  2:700786/149‑1 (MQ=255)                                                                                                                     gctgGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCa                              <  2:135839/150‑1 (MQ=255)                                                                                                                     gctgGACACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGccc                               >  1:567049/1‑149 (MQ=255)                                                                                                                        ggTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGt                          <  1:455252/151‑1 (MQ=255)                                                                                                                               ggCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGAt                    <  1:488200/150‑1 (MQ=255)                                                                                                                                gCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGt                  <  2:91200/151‑1 (MQ=255)                                                                                                                                  gATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTg                >  1:61482/1‑151 (MQ=255)                                                                                                                                   aTCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGa               <  2:38887/151‑1 (MQ=255)                                                                                                                                      ccGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaaca            <  1:35046/151‑1 (MQ=255)                                                                                                                                       cGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaacaa           <  2:335400/151‑1 (MQ=255)                                                                                                                                        ggAGATTGGAAGACGCGCGCCCCCCTTGCCGTCAATGTTAGTCTCACCGTATACTTCGGAAGAACGACGCGCTTCTGGCAGCATCCCGCGCGGAATATCCAGCCCTTCCAGCATTTTGTCGTCCCAGTCCAGGGAATGGCTGTTGaacaac          >  2:522131/1‑151 (MQ=255)                                                                                                                                            aTTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATgg      <  1:348580/151‑1 (MQ=255)                                                                                                                                               ggAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAc   <  1:26601/151‑1 (MQ=255)                                                                                                                                                gAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACg  >  1:73182/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACG  >  NC_000913/4116365‑4116658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑