Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,179,175	T→G	D99E (GAT→GAG)	rplA →	50S ribosomal subunit protein L1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,179,175	0	T	G	100.0%	189.3 / NA	50	D99E (GAT→GAG)	rplA	50S ribosomal subunit protein L1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/26);  total (24/26)

CGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGA  >  NC_000913/4179028‑4179320                                                                                                                                                    |                                                                                                                                                  cGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCt                                                                                                                                                 <  1:337521/150‑1 (MQ=255)    cGCTCGTAAATCTGACCAGAACGTATGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGc                                                                                                                                              <  2:100598/150‑1 (MQ=255)         gTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCa                                                                                                                                        <  1:113501/151‑1 (MQ=255)           aaaTCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGAcc                                                                                                                                         >  2:229427/1‑148 (MQ=255)             aTCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGAcc                                                                                                                                         >  2:898776/1‑146 (MQ=255)                tGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCa                                                                                                                                   >  1:317235/1‑149 (MQ=255)                 gACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCa                                                                                                                                   <  1:783595/148‑1 (MQ=255)                 gACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGa                                                                                                                                <  2:587408/151‑1 (MQ=255)                   ccAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGaaa                                                                                                                              <  1:190983/151‑1 (MQ=255)                   ccAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGaaa                                                                                                                              <  1:681462/151‑1 (MQ=255)                     aGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAgg                                                                                                                            <  1:601625/151‑1 (MQ=255)                        acgtacgtGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGa                                                                                                                         <  1:147467/151‑1 (MQ=255)                          gtacgtGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGaa                                                                                                                        >  2:922852/1‑150 (MQ=255)                             cgtGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGa                                                                                                                    <  1:826918/151‑1 (MQ=255)                                ggtgCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACt                                                                                                                 <  2:627807/151‑1 (MQ=255)                                       cTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATGTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACgt                                                                                                          >  2:393828/1‑151 (MQ=255)                                         gTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACgttg                                                                                                        >  1:776344/1‑151 (MQ=255)                                              gCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTa                                                                                                     <  2:82492/149‑1 (MQ=255)                                              gCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTAtt                                                                                                   >  1:62603/1‑151 (MQ=255)                                              gCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTAt                                                                                                    <  1:260766/150‑1 (MQ=255)                                               ccGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTg                                                                                                  >  1:559792/1‑151 (MQ=255)                                                 gCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCt                                                                                                >  2:678250/1‑151 (MQ=255)                                                 gCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCt                                                                                                <  1:653638/151‑1 (MQ=255)                                                      gTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTcc                                                                                           <  2:903405/151‑1 (MQ=255)                                                          tGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGAt                                                                                       >  2:246751/1‑151 (MQ=255)                                                           ggCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATg                                                                                      <  1:665566/151‑1 (MQ=255)                                                               gttccgttcGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAAt                                                                                  >  2:840710/1‑151 (MQ=255)                                                                       cgcgTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCgttg                                                                          <  1:313298/151‑1 (MQ=255)                                                                       cgcgTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCgttg                                                                          <  1:305626/151‑1 (MQ=255)                                                                          gTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGttgttg                                                                       >  1:63366/1‑151 (MQ=255)                                                                            aGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGc                                                                     <  1:487586/151‑1 (MQ=255)                                                                             gCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGcc                                                                    <  1:73956/151‑1 (MQ=255)                                                                                 tATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCt                                                                >  1:657841/1‑151 (MQ=255)                                                                                    ttACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTggg                                                             >  2:37992/1‑151 (MQ=255)                                                                                      aCCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGc                                                            <  2:476778/150‑1 (MQ=255)                                                                                       cccAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCGGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCa                                                          <  1:806023/151‑1 (MQ=255)                                                                                                 aaaCGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGt                                                >  2:887577/1‑151 (MQ=255)                                                                                                  aaCGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTc                                               <  1:577411/151‑1 (MQ=255)                                                                                                    cGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCcg                                             >  2:391652/1‑151 (MQ=255)                                                                                                     gCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCcgc                                            <  2:200078/151‑1 (MQ=255)                                                                                                      cTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCcgcg                                           >  1:177119/1‑151 (MQ=255)                                                                                                       tGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCcgcgc                                          >  2:754527/1‑151 (MQ=255)                                                                                                        gAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCcgcgcg                                         <  1:562615/151‑1 (MQ=255)                                                                                                                       gCAGGCGCAGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAAcc                          >  2:133868/1‑151 (MQ=255)                                                                                                                               aGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTg                  >  2:747521/1‑151 (MQ=255)                                                                                                                               aGAACTGGTAGGTATGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTg                  >  1:754761/1‑151 (MQ=255)                                                                                                                                     ggtaggtaTGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTAc             <  1:276112/150‑1 (MQ=255)                                                                                                                                             tGGAAGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACAGTAACAcc    >  2:637825/1‑151 (MQ=255)                                                                                                                                                aaGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGa  >  1:298498/1‑150 (MQ=255)                                                                                                                                                aaGAGCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGa  >  1:483920/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGA  >  NC_000913/4179028‑4179320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑