Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,217,768	T→G	A220A (GCT→GCG)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,768	0	T	G	100.0%	262.9 / NA	71	A220A (GCT→GCG)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/38);  total (33/38)

GATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATT  >  NC_000913/4217621‑4217915                                                                                                                                                    |                                                                                                                                                    gatgaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCgga                                                                                                                                                   <  1:551070/150‑1 (MQ=255)   tgaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCggacgt                                                                                                                                                >  2:800473/1‑151 (MQ=255)    gaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCggacgtg                                                                                                                                               >  2:311143/1‑151 (MQ=255)          gatgatTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGGCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGc                                                                                                                                         <  2:405211/151‑1 (MQ=255)          gatgatTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGc                                                                                                                                         >  1:196807/1‑151 (MQ=255)           atgatTGAAGCCGGTGCAGCGGCAGTTCACTGCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACggg                                                                                                                                          <  2:192713/149‑1 (MQ=255)              atTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTc                                                                                                                                     >  2:550427/1‑151 (MQ=255)                    gCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAAccc                                                                                                                               <  1:311151/151‑1 (MQ=255)                    gCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAAccc                                                                                                                               <  2:261938/151‑1 (MQ=255)                      cGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCctg                                                                                                                             <  1:683141/151‑1 (MQ=255)                        gTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGTCGGGCGTTCCAACCctgct                                                                                                                           <  2:205860/151‑1 (MQ=255)                          gCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTgg                                                                                                                         <  2:799347/151‑1 (MQ=255)                                gCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGccc                                                                                                                   >  2:831869/1‑151 (MQ=255)                                 cAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCg                                                                                                                  >  2:690450/1‑151 (MQ=255)                                  aGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGt                                                                                                                 <  2:640054/151‑1 (MQ=255)                                    ttCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTAc                                                                                                               >  1:270663/1‑151 (MQ=255)                                         ttCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATg                                                                                                          >  2:717343/1‑151 (MQ=255)                                           cGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGc                                                                                                         <  2:716527/150‑1 (MQ=255)                                           cGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGc                                                                                                         <  1:233548/150‑1 (MQ=255)                                              aGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGAt                                                                                                     >  1:709545/1‑151 (MQ=255)                                                  cAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                 >  2:637494/1‑151 (MQ=255)                                                   aGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcggc                                                                                                >  2:683144/1‑151 (MQ=255)                                                    gCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcggcg                                                                                               <  1:901723/151‑1 (MQ=255)                                                      tGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGcggcgg                                                                                              >  1:76836/1‑150 (MQ=255)                                                       ggCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGAt                                                                                            <  2:793932/151‑1 (MQ=255)                                                        gCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATc                                                                                           >  2:404988/1‑151 (MQ=255)                                                         cGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCt                                                                                          <  1:800491/151‑1 (MQ=255)                                                          gTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTg                                                                                         >  1:897523/1‑151 (MQ=255)                                                          gTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTg                                                                                         <  1:347446/151‑1 (MQ=255)                                                            cAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGa                                                                                        <  1:533104/150‑1 (MQ=255)                                                                gaagaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGCTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCAcc                                                                                   >  1:853167/1‑151 (MQ=255)                                                                 aagaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCAcc                                                                                   >  2:361416/1‑150 (MQ=255)                                                                 aagaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCt                                                                                  <  2:340820/151‑1 (MQ=255)                                                                   gaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTcc                                                                                >  1:703729/1‑151 (MQ=255)                                                                   gaaATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTcc                                                                                <  1:280095/151‑1 (MQ=255)                                                                    aaaTGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCg                                                                               >  1:611539/1‑151 (MQ=255)                                                                      aTGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGAt                                                                             <  1:539961/151‑1 (MQ=255)                                                                        gCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGAtt                                                                            <  1:67567/150‑1 (MQ=255)                                                                        gCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAAAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGAtt                                                                            <  2:769287/150‑1 (MQ=255)                                                                         cGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGc                                                                          <  2:537177/151‑1 (MQ=255)                                                                           gTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGc                                                                          >  2:878936/1‑149 (MQ=255)                                                                           gTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                         >  2:615342/1‑150 (MQ=255)                                                                                aTGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCg                                                                    >  2:117670/1‑150 (MQ=255)                                                                                     cggcAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGa                                                               >  1:551468/1‑150 (MQ=255)                                                                                         aaaGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCg                                                          <  2:28188/151‑1 (MQ=255)                                                                                          aaGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGa                                                         <  2:779298/151‑1 (MQ=255)                                                                                          aaGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGa                                                         <  2:231105/151‑1 (MQ=255)                                                                                           aGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGaa                                                        <  2:584041/151‑1 (MQ=255)                                                                                               ttAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAAttt                                                     >  2:182901/1‑150 (MQ=255)                                                                                                tAGTGCGAACTCAGGAAGCTCTTCAGAAACTGGTCCCGGCGCGTCTGGCAGCGGACGTGACGGGCGATCCAACCCTGCTGGTTGCCCGTACCGGTGCTGATGCGGCGGATCTCATCACCTCCGATTGCGACCCGTATGACAGCGAATtta                                                    <  2:59454/150‑1 (MQ=255)                                                                                                tAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATtta                                                    <  2:589346/150‑1 (MQ=255)                                                                                                 aGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATttatt                                                  <  2:89429/151‑1 (MQ=255)                                                                                                    gCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTAcc                                               <  1:224240/151‑1 (MQ=255)                                                                                                     ccAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCg                                              >  1:835467/1‑151 (MQ=255)                                                                                                      cAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGTGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCCAATTTATTACCg                                              >  2:874463/1‑150 (MQ=255)                                                                                                           cAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGa                                          <  1:491491/149‑1 (MQ=255)                                                                                                           cAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAg                                         >  1:270600/1‑150 (MQ=255)                                                                                                              gAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTa                                     <  1:48761/151‑1 (MQ=255)                                                                                                                    aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGt                                <  2:662030/150‑1 (MQ=255)                                                                                                                    aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTAACGGCGAGCGTACCAGTg                               >  2:59875/1‑151 (MQ=255)                                                                                                                       cAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGa                              >  1:40681/1‑149 (MQ=255)                                                                                                                       cAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAg                            <  1:878956/151‑1 (MQ=255)                                                                                                                       cAGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAg                            <  2:617059/151‑1 (MQ=255)                                                                                                                        aGAAACTGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAgg                           >  1:191511/1‑151 (MQ=255)                                                                                                                              tGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGcttctt                     >  1:737808/1‑151 (MQ=255)                                                                                                                               ggTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGcttctt                     >  2:538892/1‑150 (MQ=255)                                                                                                                                gTCGCGGCGCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTcc                   <  2:68042/151‑1 (MQ=255)                                                                                                                                     ggcgCGTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACt              >  1:359665/1‑151 (MQ=255)                                                                                                                                        gcgTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCAt           <  1:326784/151‑1 (MQ=255)                                                                                                                                          gTCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATg          >  2:50467/1‑150 (MQ=255)                                                                                                                                           tCTGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTTTTCCGTACTCATg          >  2:224828/1‑149 (MQ=255)                                                                                                                                             tGGCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCggg      >  2:881189/1‑151 (MQ=255)                                                                                                                                               gCAGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCa    <  2:256517/151‑1 (MQ=255)                                                                                                                                                 aGCGGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCAtt  <  1:615357/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATT  >  NC_000913/4217621‑4217915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑