Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,533	A→C	Y312D (TAC→GAC)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,533	0	A	C	100.0%	153.2 / NA	42	Y312D (TAC→GAC)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/15);  total (27/15)

GTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAT  >  NC_000913/4255386‑4255680                                                                                                                                                    |                                                                                                                                                    gTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGgtcgtc                                                                                                                                                  >  2:432566/1‑151 (MQ=255)  tttATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTcc                                                                                                                                                 >  1:673604/1‑151 (MQ=255)        gCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCAtgtg                                                                                                                                            <  2:6875/150‑1 (MQ=255)        gCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCAtgtg                                                                                                                                            <  2:150769/150‑1 (MQ=255)          cTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACt                                                                                                                                         >  1:847529/1‑151 (MQ=255)             tAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCg                                                                                                                                      >  1:165944/1‑151 (MQ=255)             tAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCg                                                                                                                                      >  1:383186/1‑151 (MQ=255)              aaaCGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCg                                                                                                                                      <  2:227927/150‑1 (MQ=255)               aaCGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGt                                                                                                                                    >  2:666313/1‑151 (MQ=255)                          aaTAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGcaca                                                                                                                         <  2:866979/151‑1 (MQ=255)                           aTAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACat                                                                                                                        >  1:859254/1‑151 (MQ=255)                              aaGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACatat                                                                                                                      >  2:671573/1‑150 (MQ=255)                                    gCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGc                                                                                                                >  2:515503/1‑150 (MQ=255)                                    gCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCt                                                                                                               >  2:394201/1‑151 (MQ=255)                                          aGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGAc                                                                                                         <  2:393483/151‑1 (MQ=255)                                            aCGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACcg                                                                                                       <  1:490000/151‑1 (MQ=255)                                                                 aGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAAcg                                                                                  >  2:296682/1‑151 (MQ=255)                                                                    ccAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACgcgc                                                                               >  2:154931/1‑151 (MQ=255)                                                                      aGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTc                                                                             >  1:412342/1‑151 (MQ=255)                                                                        aaaTTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTc                                                                             >  1:734539/1‑149 (MQ=255)                                                                              aGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTAt                                                                     <  1:194110/151‑1 (MQ=255)                                                                                      cccGGCGGCGATATGCGGCGGCGCCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGtt                                                              <  1:283919/150‑1 (MQ=255)                                                                                         ggcggcGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATg                                                          <  1:641207/151‑1 (MQ=255)                                                                                             gcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCt                                                      <  1:409598/151‑1 (MQ=255)                                                                                             gcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCt                                                      <  1:669426/151‑1 (MQ=255)                                                                                               gATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTgg                                                    >  2:355347/1‑151 (MQ=255)                                                                                                           cACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTc                                        >  2:37539/1‑151 (MQ=255)                                                                                                            aCCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc                                       >  2:116971/1‑151 (MQ=255)                                                                                                             ccAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc                                       >  1:814218/1‑150 (MQ=255)                                                                                                                  cccTGGTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg                                  >  2:863548/1‑150 (MQ=255)                                                                                                                      ggTGATACAGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGc                              <  1:465835/150‑1 (MQ=255)                                                                                                                              aGCACGTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATAc                     <  2:379609/151‑1 (MQ=255)                                                                                                                                   gTAAGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtc                 >  2:1498/1‑150 (MQ=255)                                                                                                                                     aaGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcag               <  1:683996/150‑1 (MQ=255)                                                                                                                                      aGAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtc             >  2:571348/1‑151 (MQ=255)                                                                                                                                       gAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtca            <  1:729508/151‑1 (MQ=255)                                                                                                                                       gAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtc             >  2:848216/1‑150 (MQ=255)                                                                                                                                       gAAAGCAGCAGGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagt              >  2:81808/1‑149 (MQ=255)                                                                                                                                            cagcagGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCg        >  2:817496/1‑150 (MQ=255)                                                                                                                                             agcagGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa       >  1:129142/1‑150 (MQ=255)                                                                                                                                             agcagGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa       >  1:869868/1‑150 (MQ=255)                                                                                                                                               cagGTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatc    >  1:781588/1‑151 (MQ=255)                                                                                                                                                 ggTCGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat  >  2:716371/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAT  >  NC_000913/4255386‑4255680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑