Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,304,733 T→G K622T (AAA→ACA)  yjcS ← metallo‑beta‑lactamase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,304,7330TG98.5% 247.0 / ‑3.7 65K622T (AAA→ACA) yjcSmetallo‑beta‑lactamase superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (35/29);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TTCAGCGCCCCCAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTT  >  NC_000913/4304586‑4304878
                                                                                                                                                   |                                                                                                                                                 
ttCAGCGCCCCCAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTgg                                                                                                                                                >  2:86440/1‑151 (MQ=255)
 tCAGCGCCCCCAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATtgtg                                                                                                                                                 <  2:854358/149‑1 (MQ=255)
  cAGCGCCCCCAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCt                                                                                                                                              <  1:693672/151‑1 (MQ=255)
       cccccAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCAATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTcc                                                                                                                                         <  2:705899/151‑1 (MQ=255)
        ccccAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCg                                                                                                                                        >  1:476715/1‑151 (MQ=255)
        ccccAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCg                                                                                                                                        <  1:528462/151‑1 (MQ=255)
          ccAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCgg                                                                                                                                       >  2:462089/1‑150 (MQ=255)
             aaaGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGt                                                                                                                                      >  1:172340/1‑148 (MQ=255)
             aaaGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTc                                                                                                                                     >  1:19361/1‑149 (MQ=255)
                     tGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCg                                                                                                                            >  1:388427/1‑150 (MQ=255)
                           tAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTg                                                                                                                          >  1:266756/1‑146 (MQ=255)
                            aaTTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGAt                                                                                                                     <  1:731979/150‑1 (MQ=255)
                             aTTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCt                                                                                                                   >  1:613506/1‑151 (MQ=255)
                               ttGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTGCGGTCCTCACGGCGGGCAGGTc                                                                                                                    >  2:532479/1‑148 (MQ=255)
                                tGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCtt                                                                                                                  >  2:772811/1‑149 (MQ=255)
                                   ggTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACgg                                                                                                             >  2:251385/1‑151 (MQ=255)
                                     ttACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGc                                                                                                            >  1:564954/1‑150 (MQ=255)
                                       aCGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTg                                                                                                          <  1:472588/150‑1 (MQ=255)
                                         gATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGAt                                                                                                        >  2:641525/1‑150 (MQ=255)
                                            atTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTACAGATCTTCACGGCTGATGTa                                                                                                     <  1:658631/150‑1 (MQ=255)
                                                   cacaAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAgg                                                                                             <  2:566347/151‑1 (MQ=255)
                                                       aaTCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCCGATCTTCACGGCTGATGTAGAAAGAgg                                                                                             >  2:859135/1‑147 (MQ=255)
                                                        aTCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCg                                                                                        <  2:164298/151‑1 (MQ=255)
                                                        aTCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGAACTTCACGGCCGATTTAGAAAGAggggccg                                                                                        >  2:338651/1‑146 (MQ=255)
                                                          cGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCgg                                                                                       >  2:532903/1‑150 (MQ=255)
                                                          cGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCgg                                                                                       >  1:688819/1‑150 (MQ=255)
                                                           gAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGGAGAAAGAGGcggcgg                                                                                       >  2:180742/1‑149 (MQ=255)
                                                             aaTTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGGGTGCAGATCTTCACGGCTGTTGTAGAAAGAGGGGTCg                                                                                        >  2:143641/1‑146 (MQ=255)
                                                                     aGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGc                                                                            <  2:538087/150‑1 (MQ=255)
                                                                      gACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGtt                                                                              >  1:438075/1‑147 (MQ=255)
                                                                         aGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCg                                                                        >  1:922662/1‑150 (MQ=255)
                                                                                aaTTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCgg                                                                <  1:544367/151‑1 (MQ=255)
                                                                                  tttCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGt                                                               <  2:190196/150‑1 (MQ=255)
                                                                                     cttcCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGtt                                                           >  2:773843/1‑151 (MQ=255)
                                                                                          aGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCa                                                      <  2:821280/151‑1 (MQ=255)
                                                                                             ttCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAAAGGCGTCGGCTTGCGGTTGCCGCGTTTTCCGGTAGTTAAGCACg                                                    >  2:469210/1‑150 (MQ=255)
                                                                                              tCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGGAGTTAAGc                                                       >  1:684637/1‑146 (MQ=255)
                                                                                               cgcgCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCt                                                  <  2:225326/150‑1 (MQ=255)
                                                                                                gcgcCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTAt                                                <  2:446190/151‑1 (MQ=255)
                                                                                                 cgcCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGGTTTCCGGGAGTTAAGCACGCTAt                                                >  1:206919/1‑150 (MQ=255)
                                                                                                 cgcCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGCGCAGCACGGCGCGCAGATCTTCCCGGCTGGTGGAGAAAGAGGGGTCGGCTTGCGGGTGCAGCGGTTTTCCGTGGTTGAACAAGCTa                                                 >  2:449271/1‑149 (MQ=255)
                                                                                                       tGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCa                                          <  2:317665/150‑1 (MQ=255)
                                                                                                        gCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAg                                         <  2:388419/150‑1 (MQ=255)
                                                                                                        gCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAg                                         <  1:883313/150‑1 (MQ=255)
                                                                                                         ccAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGc                                        <  2:233589/150‑1 (MQ=255)
                                                                                                            ataatTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGGTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTc                                     >  1:523276/1‑150 (MQ=255)
                                                                                                            ataatTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGCGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCCGCGTTTTCCGGGAGGTAAGCCCCCTATCGTTCCGCGt                                      >  1:192942/1‑149 (MQ=255)
                                                                                                              aatTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCCTGCGGGTGCAGCGTTTTCCGGGAGTTAAGCACGCTATCGTTCAGCGTCAgg                                  >  2:492726/1‑151 (MQ=255)
                                                                                                                  ttGGCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTg                               >  1:317501/1‑150 (MQ=255)
                                                                                                                    ggCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATTTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGGTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTTAGCGTCAGGTTGTgg                            >  2:252262/1‑151 (MQ=255)
                                                                                                                    ggCTTTCTTCGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAg                             <  1:86441/150‑1 (MQ=255)
                                                                                                                         tcttcGCTTTTACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGtt                          >  2:529999/1‑148 (MQ=255)
                                                                                                                               cTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGtt                 >  1:337877/1‑151 (MQ=255)
                                                                                                                                 tttACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGtt                 <  1:714496/149‑1 (MQ=255)
                                                                                                                                 tttACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGtt                 <  2:742079/149‑1 (MQ=255)
                                                                                                                                 tttACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTg                <  1:644762/150‑1 (MQ=255)
                                                                                                                                 tttACCAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTg                <  1:430853/150‑1 (MQ=255)
                                                                                                                                   tcccAGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCCCGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCt              <  1:599214/148‑1 (MQ=255)
                                                                                                                                       aGATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCatat         >  1:583514/1‑151 (MQ=255)
                                                                                                                                        gATCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATAtt        <  1:175401/151‑1 (MQ=255)
                                                                                                                                          tCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATAtt        <  1:350275/149‑1 (MQ=255)
                                                                                                                                          tCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTg       <  2:172340/150‑1 (MQ=255)
                                                                                                                                          tCCGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTg       <  2:914499/150‑1 (MQ=255)
                                                                                                                                           ccGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGa      <  1:253948/150‑1 (MQ=255)
                                                                                                                                           ccGCCATTGTGGCTTGTCCGGTCAGCACGGCGTGCAAATGTTCACCGCTGGTGTAGAAAGAGGCGTTGGCTTGCGGTTGCCGCGTTTTCCGGGAGGTAAGCACGCTATCGTTCAGCGACAGGTTGAGGGTATCGCCGTTGCTCATAtt        >  2:93482/1‑148 (MQ=255)
                                                                                                                                                aTTGTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGGAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGGTATCGCCGTTGCTCATATTGAAGtt  >  1:577739/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTCAGCGCCCCCAAAAGCGAGTGACATTAATTTGGGGTTACGATATTCACCCACAAATCGAAATTATCCAGACAGGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTT  >  NC_000913/4304586‑4304878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑