Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	435,380	A→C	K82Q (AAA→CAA)	nusB →	transcription antitermination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	435,380	0	A	C	100.0%	212.3 / NA	54	K82Q (AAA→CAA)	nusB	transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/34);  total (20/34)

AGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAA  >  NC_000913/435234‑435522                                                                                                                                                   |                                                                                                                                               aGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGc                                                                                                                                            >  2:857064/1‑151 (MQ=255) aGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGc                                                                                                                                            >  1:661509/1‑151 (MQ=255)   ttCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGc                                                                                                                                            >  1:415333/1‑149 (MQ=255)     ccTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTa                                                                                                                                        <  1:211881/151‑1 (MQ=255)      cTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTAc                                                                                                                                       >  1:53971/1‑151 (MQ=255)        ggCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACt                                                                                                                                      <  1:877812/150‑1 (MQ=255)        ggCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACt                                                                                                                                      <  1:725817/150‑1 (MQ=255)               cAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTg                                                                                                                              <  2:790601/151‑1 (MQ=255)                    tGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCt                                                                                                                          <  1:689888/150‑1 (MQ=255)                       aaaaGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTAc                                                                                                                      >  1:891301/1‑151 (MQ=255)                       aaaaGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTAc                                                                                                                      <  2:40068/151‑1 (MQ=255)                        aaaGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACg                                                                                                                     <  2:631420/151‑1 (MQ=255)                        aaaGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACg                                                                                                                     >  2:578207/1‑151 (MQ=255)                              gTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGt                                                                                                               >  1:9602/1‑151 (MQ=255)                                tGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCt                                                                                                             >  2:174099/1‑151 (MQ=255)                                    gTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAAc                                                                                                         <  2:382521/151‑1 (MQ=255)                                       cggTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTa                                                                                                      <  1:360744/149‑1 (MQ=255)                                       cTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGt                                                                                                       >  2:558724/1‑150 (MQ=255)                                         gtgcTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATCCCTCTACGGACTGATAAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGt                                                                                                       <  2:492021/145‑1 (MQ=255)                                         gTACTTCCGTGGGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAg                                                                                                     <  2:205636/150‑1 (MQ=255)                                            cTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGAt                                                                                                 >  1:208978/1‑151 (MQ=255)                                             ttCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGAtg                                                                                                >  1:429802/1‑151 (MQ=255)                                               ccGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGAtgt                                                                                               <  2:283243/150‑1 (MQ=255)                                                        gctgGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACaa                                                                                      <  2:787592/150‑1 (MQ=255)                                                           ggCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTg                                                                                  <  2:96626/151‑1 (MQ=255)                                                               ggggTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGcc                                                                               >  1:676101/1‑150 (MQ=255)                                                               ggggTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCa                                                                              <  2:737639/151‑1 (MQ=255)                                                                  gTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTa                                                                           <  1:116677/151‑1 (MQ=255)                                                                     gCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACg                                                                        <  1:603324/151‑1 (MQ=255)                                                                       gACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGaa                                                                      <  2:181573/151‑1 (MQ=255)                                                                        aCTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAg                                                                     <  1:766064/151‑1 (MQ=255)                                                                           aaTCCCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGa                                                                  <  2:666175/151‑1 (MQ=255)                                                                           aaTACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGa                                                                  <  2:705197/151‑1 (MQ=255)                                                                               ccGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGa                                                              <  2:436961/151‑1 (MQ=255)                                                                                cGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGaa                                                             <  2:694694/151‑1 (MQ=255)                                                                                   aTACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTg                                                          <  1:847037/151‑1 (MQ=255)                                                                                                   atgaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGgcg                                          >  1:386840/1‑151 (MQ=255)                                                                                                     gaAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGgcgc                                         <  1:337618/150‑1 (MQ=255)                                                                                                      aaGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGGAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGgcgc                                         >  2:571347/1‑149 (MQ=255)                                                                                                       aGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCaga                                      <  2:770405/151‑1 (MQ=255)                                                                                                         ccATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCagaag                                    <  1:713667/151‑1 (MQ=255)                                                                                                         ccATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCaga                                      >  1:705896/1‑149 (MQ=255)                                                                                                          cATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCaga                                      >  1:368834/1‑148 (MQ=255)                                                                                                              ccTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAg                                <  2:222364/150‑1 (MQ=255)                                                                                                                   cccGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATaa                          <  1:494088/151‑1 (MQ=255)                                                                                                                    ccGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATa                           >  2:335023/1‑149 (MQ=255)                                                                                                                    ccGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAg                         <  2:531675/151‑1 (MQ=255)                                                                                                                     cGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGt                        <  2:393090/151‑1 (MQ=255)                                                                                                                     cGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGt                        <  2:820633/151‑1 (MQ=255)                                                                                                                        ctgctgGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCg                     >  1:117605/1‑151 (MQ=255)                                                                                                                            tgGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCa                  >  2:630922/1‑150 (MQ=255)                                                                                                                            tgGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCa                  >  2:799723/1‑150 (MQ=255)                                                                                                                             ggAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAAc                <  1:749123/151‑1 (MQ=255)                                                                                                                                           aGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATaa  <  2:891281/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               AGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAA  >  NC_000913/435234‑435522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑