Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,687	T→G	I208S (ATC→AGC)	amiB →	N‑acetylmuramoyl‑l‑alanine amidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,687	0	T	G	100.0%	215.4 / NA	62	I208S (ATC→AGC)	amiB	N‑acetylmuramoyl‑l‑alanine amidase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/30);  total (32/30)

AATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATG  >  NC_000913/4396539‑4396830                                                                                                                                                     |                                                                                                                                                aaTCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCg                                                                                                                                               <  2:636523/151‑1 (MQ=255) aaTACGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCg                                                                                                                                               >  2:609803/1‑151 (MQ=255)    ccGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCCAGCgggc                                                                                                                                            >  2:300418/1‑149 (MQ=255)     cGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGccc                                                                                                                                           >  2:772675/1‑151 (MQ=255)       tttAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGcccc                                                                                                                                          >  2:908067/1‑150 (MQ=255)       tttAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCg                                                                                                                                         >  1:877886/1‑151 (MQ=255)        ttAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCg                                                                                                                                         <  2:708849/150‑1 (MQ=255)         tAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCgg                                                                                                                                        >  1:551934/1‑150 (MQ=255)            aaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCggtgg                                                                                                                                     >  2:173900/1‑150 (MQ=255)                  aaGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCgg                                                                                                                              <  1:61462/151‑1 (MQ=255)                   aGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCggg                                                                                                                             >  1:713180/1‑151 (MQ=255)                    gTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGga                                                                                                                            <  2:773334/151‑1 (MQ=255)                     tAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCggg                                                                                                                             >  1:303219/1‑149 (MQ=255)                          gCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGaaaaa                                                                                                                      <  2:723108/151‑1 (MQ=255)                          gCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGaaaa                                                                                                                       >  2:782033/1‑150 (MQ=255)                           cACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGaaaaa                                                                                                                      >  1:95543/1‑150 (MQ=255)                            aCTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAAt                                                                                                                     >  1:663152/1‑150 (MQ=255)                              tACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTc                                                                                                                  >  2:675466/1‑151 (MQ=255)                               aCGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTc                                                                                                                  <  1:707042/150‑1 (MQ=255)                                cGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAc                                                                                                                >  2:751418/1‑151 (MQ=255)                                          cagcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatcgccatc                                                                                                      >  1:189826/1‑151 (MQ=255)                                              agTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATcgcg                                                                                                   >  2:614225/1‑150 (MQ=255)                                               gTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATcgcgcg                                                                                                 >  2:885793/1‑151 (MQ=255)                                                tAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGGGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGt                                                                                                >  2:205823/1‑151 (MQ=255)                                                tAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGGCAGGACCCTTGCGCTAGCGGCCCCGGGGGTAAGCGGGAGAAAAATGTCACCATCGCCCTcgcgc                                                                                                  >  2:435951/1‑149 (MQ=255)                                                    aCGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTaaa                                                                                             <  1:761104/150‑1 (MQ=255)                                                     cGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAtt                                                                                           <  2:772341/151‑1 (MQ=255)                                                     cGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTACCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAtt                                                                                           <  2:228586/151‑1 (MQ=255)                                                       gTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAtt                                                                                           <  2:651352/149‑1 (MQ=255)                                                          aCGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCg                                                                                        >  2:21412/1‑149 (MQ=255)                                                              gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACttt                                                                                  >  2:451324/1‑151 (MQ=255)                                                                ccGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAAAATCACCATCGCCATCGCGCGGAAATTGCGATCttt                                                                                  >  2:489959/1‑149 (MQ=255)                                                                 cGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCt                                                                               >  2:141677/1‑151 (MQ=255)                                                                     aGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAAt                                                                           >  1:442301/1‑151 (MQ=255)                                                                     aGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAAt                                                                           <  1:206462/151‑1 (MQ=255)                                                                       cgcgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGa                                                                         <  2:619889/151‑1 (MQ=255)                                                                        gcgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGAc                                                                        >  1:825450/1‑151 (MQ=255)                                                                          gcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGa                                                                      <  2:831289/151‑1 (MQ=255)                                                                             cgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATcc                                                                   <  1:725820/151‑1 (MQ=255)                                                                                  gCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGt                                                              <  1:93505/151‑1 (MQ=255)                                                                                     aaCACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGtt                                                             <  1:413854/149‑1 (MQ=255)                                                                                               aTAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGtttt                                                 >  1:431206/1‑151 (MQ=255)                                                                                                 aaaaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTaa                                               <  1:60569/151‑1 (MQ=255)                                                                                                   aaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAAcc                                             >  1:10393/1‑151 (MQ=255)                                                                                                   aaTTATCATCGCTATTGATGCCGGACACGGCGGTAAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAAcc                                             <  1:627363/151‑1 (MQ=255)                                                                                                         catcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGAc                                       >  1:802980/1‑151 (MQ=255)                                                                                                            cGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACggg                                    <  1:536891/151‑1 (MQ=255)                                                                                                                aTTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGAtt                                <  1:909635/151‑1 (MQ=255)                                                                                                                    aTGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACtt                            >  2:616959/1‑151 (MQ=255)                                                                                                                         ggACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtct                       <  2:280396/151‑1 (MQ=255)                                                                                                                         ggACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtct                       <  1:130175/151‑1 (MQ=255)                                                                                                                          gACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtctc                      >  1:807693/1‑151 (MQ=255)                                                                                                                           acacGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCg                     <  2:410418/151‑1 (MQ=255)                                                                                                                              cggcggTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTg                  <  2:783631/151‑1 (MQ=255)                                                                                                                              cggcggTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTg                  <  2:890081/151‑1 (MQ=255)                                                                                                                                 cggTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATg               <  1:376872/151‑1 (MQ=255)                                                                                                                                     cAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGgc           <  1:326040/151‑1 (MQ=255)                                                                                                                                        gACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGgcgc         >  1:252233/1‑150 (MQ=255)                                                                                                                                         aCCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGgcgc         >  2:231052/1‑149 (MQ=255)                                                                                                                                              ggCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGa    <  2:51643/149‑1 (MQ=255)                                                                                                                                              ggCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGa    <  2:879819/149‑1 (MQ=255)                                                                                                                                              ggCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGAtg  <  1:614240/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                AATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATG  >  NC_000913/4396539‑4396830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑