Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,399,742 | A→C | D164A (GAT→GCT) | miaA → | delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,399,742 | 0 | A | C | 98.3% | 219.7 / ‑4.6 | 59 | D164A (GAT→GCT) | miaA | delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase |
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (24/34); total (24/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
GCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACG > NC_000913/4399596‑4399889 | gCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCca < 1:10705/151‑1 (MQ=255) gCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGACATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTcc > 2:592129/1‑150 (MQ=255) ggAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCcaca < 2:509782/150‑1 (MQ=255) gAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACaaa < 1:71149/151‑1 (MQ=255) aaGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACaa > 1:282468/1‑149 (MQ=255) aGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAgg > 1:505280/1‑151 (MQ=255) ttGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCttt > 1:900318/1‑151 (MQ=255) tCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTccc > 2:83289/1‑151 (MQ=255) cgcTACCGTCGGCAGAGCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGATGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGc > 2:199873/1‑151 (MQ=255) cTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCAc > 1:511827/1‑151 (MQ=255) aCCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTg < 2:351810/151‑1 (MQ=255) ccGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTgg > 1:924438/1‑151 (MQ=255) gTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGaa < 2:77348/151‑1 (MQ=255) gTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGaa < 2:526374/151‑1 (MQ=255) gTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGaa < 2:284418/151‑1 (MQ=255) gTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGaa < 2:226304/151‑1 (MQ=255) gTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGa > 2:345063/1‑150 (MQ=255) cGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAg < 2:878352/150‑1 (MQ=255) aGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGttt < 2:197155/149‑1 (MQ=255) gACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGtttttt < 1:62398/151‑1 (MQ=255) gACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGtttttt < 1:467381/151‑1 (MQ=255) cccGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTc > 2:68516/1‑151 (MQ=255) ccGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCa < 1:282569/151‑1 (MQ=255) cGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCAt < 2:848578/151‑1 (MQ=255) aGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCg < 1:587108/151‑1 (MQ=255) cGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTa < 2:181904/151‑1 (MQ=255) cAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACt < 2:356610/151‑1 (MQ=255) aaTTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACttt > 2:469057/1‑150 (MQ=255) aGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGAc > 1:267333/1‑151 (MQ=255) ggcggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACg > 1:628531/1‑151 (MQ=255) agagCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACg < 2:260807/151‑1 (MQ=255) cAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTc > 1:305134/1‑149 (MQ=255) cAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAg < 1:922151/151‑1 (MQ=255) tGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGAc > 2:370952/1‑150 (MQ=255) gAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGctc < 1:339470/151‑1 (MQ=255) gTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGctct > 2:794857/1‑150 (MQ=255) aTTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCg < 1:847319/151‑1 (MQ=255) tGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGGGACGCTCTACCGt < 2:328898/150‑1 (MQ=255) tGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGt > 1:837029/1‑150 (MQ=255) aTCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATc > 1:18056/1‑150 (MQ=255) aTCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCa < 1:592144/151‑1 (MQ=255) cAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGc < 2:481566/151‑1 (MQ=255) aCTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCAt < 2:756980/151‑1 (MQ=255) cAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAg < 2:636065/150‑1 (MQ=255) aggaggTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGtt > 1:886069/1‑151 (MQ=255) ggTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGcc < 2:665175/151‑1 (MQ=255) gTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCa < 1:262388/151‑1 (MQ=255) aGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATc > 1:801396/1‑151 (MQ=255) gATCCGGTTGCGGGAGCAAGGATACATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTTCCGTATCAGGTGCAACAGTACGCCATCg < 2:715935/151‑1 (MQ=255) ccGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGccc > 1:119537/1‑151 (MQ=255) ggTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCg > 1:742574/1‑151 (MQ=255) tGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc < 1:742482/150‑1 (MQ=255) gcaAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTg < 1:83289/151‑1 (MQ=255) aaGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGa > 2:37411/1‑150 (MQ=255) aaGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGa > 1:737953/1‑150 (MQ=255) aGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAAc < 1:446244/151‑1 (MQ=255) ggATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACt < 1:68516/151‑1 (MQ=255) cAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCa < 1:551225/151‑1 (MQ=255) aTGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACg < 1:140847/151‑1 (MQ=255) | GCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACG > NC_000913/4399596‑4399889 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |