Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,535,814	T→G	pseudogene (812/1029 nt)	yjhR →	pseudogene, helicase family; putative frameshift suppressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,535,814	0	T	G	100.0%	203.6 / NA	54	pseudogene (812/1029 nt)	yjhR	pseudogene, helicase family; putative frameshift suppressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/25);  total (29/25)

AAACGGGATTTCTGGCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCTCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATAAGTATCAACGATACGATACGTCTT  >  NC_000913/4535667‑4535957                                                                                                                                                    |                                                                                                                                                aaaCGGGATTTCTGGCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCa                                                                                                                                               >  1:808079/1‑150 (MQ=255) aaaCGGGATTTCTGGCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCa                                                                                                                                               >  1:309079/1‑150 (MQ=255)  aaCGGGATTTCTGGCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAAt                                                                                                                                             >  1:217565/1‑151 (MQ=255)    cGGGATTTCTGGCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATaa                                                                                                                                           <  2:110312/151‑1 (MQ=255)      ggATTTCTGGCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATa                                                                                                                                            >  1:134856/1‑148 (MQ=255)              ggCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTa                                                                                                                                   <  2:698372/149‑1 (MQ=255)                       ggCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGaaa                                                                                                                        <  1:508335/151‑1 (MQ=255)                       ggCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGaa                                                                                                                         <  2:495388/150‑1 (MQ=255)                              gCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGtt                                                                                                                  >  2:406510/1‑150 (MQ=255)                                 cGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATc                                                                                                              <  1:406519/151‑1 (MQ=255)                                        ggggCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTc                                                                                                        >  1:218971/1‑150 (MQ=255)                                             aTTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGc                                                                                                   >  1:372563/1‑150 (MQ=255)                                               tGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCaa                                                                                                 >  1:526327/1‑150 (MQ=255)                                                   atTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATtg                                                                                            <  1:275251/151‑1 (MQ=255)                                                     tACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATtgt                                                                                           <  2:481497/150‑1 (MQ=255)                                                          tggtgACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTg                                                                                      >  2:132670/1‑150 (MQ=255)                                                           ggtgACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGa                                                                                     <  2:245083/150‑1 (MQ=255)                                                              gACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGa                                                                                  <  1:59309/150‑1 (MQ=255)                                                                  gATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATgaag                                                                             <  1:761249/151‑1 (MQ=255)                                                                  gATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATgaa                                                                              <  1:414406/150‑1 (MQ=255)                                                                      aaaaCTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAAct                                                                         >  2:128593/1‑151 (MQ=255)                                                                       aaaCTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAActg                                                                        >  2:869896/1‑151 (MQ=255)                                                                         aCTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAActgct                                                                      <  1:613681/151‑1 (MQ=255)                                                                         aCTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAActgct                                                                      >  2:836935/1‑151 (MQ=255)                                                                                tataGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTc                                                               >  2:588178/1‑151 (MQ=255)                                                                                    gcgcATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGAt                                                           <  1:390681/151‑1 (MQ=255)                                                                                      gcATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCt                                                         <  2:802172/151‑1 (MQ=255)                                                                                         tGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCtttt                                                      >  1:691577/1‑151 (MQ=255)                                                                                          gTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCtttt                                                      <  1:442559/150‑1 (MQ=255)                                                                                          gTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCtttt                                                      <  1:388903/150‑1 (MQ=255)                                                                                            cGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAAc                                                   >  1:850717/1‑151 (MQ=255)                                                                                            cGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAAc                                                   >  2:877789/1‑151 (MQ=255)                                                                                               tgatgatAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTgg                                                >  2:917102/1‑151 (MQ=255)                                                                                               tgatgatAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTgg                                                >  1:702218/1‑151 (MQ=255)                                                                                               tgatgatAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTgg                                                >  1:445561/1‑151 (MQ=255)                                                                                                gatgatAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGt                                               <  1:197184/151‑1 (MQ=255)                                                                                                   gatAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTc                                             >  1:145109/1‑150 (MQ=255)                                                                                                    atAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCa                                            <  1:480380/150‑1 (MQ=255)                                                                                                       aGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGc                                        >  1:246067/1‑151 (MQ=255)                                                                                                       aGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGc                                        >  1:308759/1‑151 (MQ=255)                                                                                                            cAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGTAGAACTGCTGTGTGTCGGATCTTTTAGCTGGTTCAGTGCTacac                                   >  2:621318/1‑151 (MQ=255)                                                                                                            cAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTaca                                    <  2:201329/150‑1 (MQ=255)                                                                                                             aGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACg                                  >  2:244381/1‑151 (MQ=255)                                                                                                              ggAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACgg                                 <  2:557107/151‑1 (MQ=255)                                                                                                               gagaAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACggg                                >  2:486441/1‑151 (MQ=255)                                                                                                               gagaAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACggg                                <  1:639266/151‑1 (MQ=255)                                                                                                                agaAACAACATCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACggg                                >  2:709756/1‑150 (MQ=255)                                                                                                                      aacaTCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATa                          >  2:160995/1‑150 (MQ=255)                                                                                                                         aTCTGCTGAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATAAGt                       >  2:640687/1‑150 (MQ=255)                                                                                                                               tgAATGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTCCAGTGCTACACGGGAAGATAAGTATCAac                 >  1:506533/1‑150 (MQ=255)                                                                                                                                 aaTGATGCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATAAGTATCAacgat              <  1:74364/151‑1 (MQ=255)                                                                                                                                     atgCTGTTGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATAAGTATCAacgatacga          <  1:548397/151‑1 (MQ=255)                                                                                                                                           ttGAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATAAGTATCAACGATACGATACGt     <  1:77528/150‑1 (MQ=255)                                                                                                                                             gAAAAGCGCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATAAGTATCAACGATACGATACGTCtt  <  2:191992/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                AAACGGGATTTCTGGCATCAATGGCTTTGGCCCGTTCCCGGGGCATTGATATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCATGTCGATGATGATAAGCGTCAGGAGAAACAACATCTGCTGAATGATGCTGTTGAAAAGCTCAATAAGATGGGTATCGCTACGAAATTGGTTAATCGTGTTCACAGCAAAATTGTGATTGAGGATGAAGAACTGCTGTGTGTCGGATCTTTTAACTGGTTCAGTGCTACACGGGAAGATAAGTATCAACGATACGATACGTCTT  >  NC_000913/4535667‑4535957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑