Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,543,958	T→G	V77V (GTT→GTG)	fimI →	fimbrial protein involved in type 1 pilus biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,543,958	0	T	G	100.0%	213.9 / NA	55	V77V (GTT→GTG)	fimI	fimbrial protein involved in type 1 pilus biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/25);  total (30/25)

CCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTTATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGA  >  NC_000913/4543810‑4544101                                                                                                                                                     |                                                                                                                                                ccGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGAt                                                                                                                                               >  1:745033/1‑151 (MQ=255)  cggcggAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGAtt                                                                                                                                              >  1:390821/1‑151 (MQ=255)       gAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCAttt                                                                                                                                         <  2:308649/151‑1 (MQ=255)           tatGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACgg                                                                                                                                     >  2:652498/1‑151 (MQ=255)              gCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGaa                                                                                                                                  <  1:530819/151‑1 (MQ=255)                aaTTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATg                                                                                                                                >  2:468146/1‑151 (MQ=255)                      aGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCAc                                                                                                                          >  2:788793/1‑151 (MQ=255)                        ggCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACgg                                                                                                                        >  1:190984/1‑151 (MQ=255)                         gCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACggt                                                                                                                       <  2:681691/151‑1 (MQ=255)                             cATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGgtggtg                                                                                                                   >  1:288284/1‑151 (MQ=255)                                 attGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGgtgagtg                                                                                                               >  1:702581/1‑151 (MQ=255)                                    gCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGaa                                                                                                             <  1:178847/150‑1 (MQ=255)                                    gCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAAc                                                                                                            >  1:591922/1‑151 (MQ=255)                                      ggAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACgt                                                                                                          <  1:856352/151‑1 (MQ=255)                                      ggAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACgt                                                                                                          <  2:75931/151‑1 (MQ=255)                                      ggAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACgt                                                                                                          >  1:697095/1‑151 (MQ=255)                                       gAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACgtg                                                                                                         >  2:132899/1‑151 (MQ=255)                                        aaaCTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACgtgt                                                                                                        <  2:693550/151‑1 (MQ=255)                                        aaaCTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACgtgt                                                                                                        <  1:282377/151‑1 (MQ=255)                                             tGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAgg                                                                                                     <  1:808965/149‑1 (MQ=255)                                                 ggATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGc                                                                                               >  1:351966/1‑151 (MQ=255)                                                      gAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGttt                                                                                           >  2:558378/1‑150 (MQ=255)                                                      gAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTc                                                                                          >  1:288900/1‑151 (MQ=255)                                                         gCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACg                                                                                       <  1:194557/151‑1 (MQ=255)                                                              tGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTc                                                                                  >  1:905034/1‑151 (MQ=255)                                                                 tAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTcgcg                                                                               >  2:199702/1‑151 (MQ=255)                                                                   aaCAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCgg                                                                              >  2:675383/1‑150 (MQ=255)                                                                   aaCAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCgg                                                                              >  1:527666/1‑150 (MQ=255)                                                                   aaCAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGa                                                                             <  1:461418/151‑1 (MQ=255)                                                                        aggACGGTCAAGATGGGGCAAATCAGCAGTAACAGGTTTCATGCGGTTGGGGAAGATAGAGAACCGGTGACGAGGGGGATTCATTTACGGGAATGTAGCAGGGTGGTGAGTGAACGTGTAGGTGGGGCGTTTCACGGTGTCGCGGAGGGt                                                                         >  2:896110/3‑150 (MQ=255)                                                                                   tatGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGAt                                                             >  1:710978/1‑151 (MQ=255)                                                                                        ggCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCt                                                        >  2:828243/1‑151 (MQ=255)                                                                                             aTCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCg                                                   <  1:523470/151‑1 (MQ=255)                                                                                                 gcagTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTggg                                               >  1:575327/1‑151 (MQ=255)                                                                                                  cagTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCTCCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGga                                              <  2:351958/151‑1 (MQ=255)                                                                                                  cagTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGga                                              <  2:85146/151‑1 (MQ=255)                                                                                                    gTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGgaga                                            <  1:634407/151‑1 (MQ=255)                                                                                                         cGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGc                                       <  2:326236/151‑1 (MQ=255)                                                                                                          ggTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGcc                                      <  2:361860/151‑1 (MQ=255)                                                                                                          ggTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGcc                                      >  1:554739/1‑151 (MQ=255)                                                                                                               cATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGa                                 <  2:190522/151‑1 (MQ=255)                                                                                                                     gTTGGGGAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGcc                            >  2:743343/1‑150 (MQ=255)                                                                                                                        ggggAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGccacca                        <  1:723795/151‑1 (MQ=255)                                                                                                                         gggAAGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGccacca                        >  1:808973/1‑150 (MQ=255)                                                                                                                             aGATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATAtt                   >  2:913701/1‑151 (MQ=255)                                                                                                                              gATAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTg                  <  1:332525/151‑1 (MQ=255)                                                                                                                               aTAGCGCACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTgg                 <  1:677924/151‑1 (MQ=255)                                                                                                                                  gcgcACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGt              >  1:16763/1‑151 (MQ=255)                                                                                                                                   cgcACCGGTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTa             <  2:376007/151‑1 (MQ=255)                                                                                                                                          gTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCgttgt      <  2:766439/151‑1 (MQ=255)                                                                                                                                          gTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCgttgt      >  2:257234/1‑151 (MQ=255)                                                                                                                                          gTGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCgttgt      <  2:841574/151‑1 (MQ=255)                                                                                                                                           tGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCgttgtt     >  1:45215/1‑151 (MQ=255)                                                                                                                                           tGCCTTTTGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCgttgtt     >  1:877290/1‑151 (MQ=255)                                                                                                                                                tttGTGATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTtga  <  2:11011/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                CCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCCGGTGATAAACAAATGACGGTCAATATGGGGCAAATCAGCAGTAACCGGTTTCATGCGGTTGGGGAAGATAGCGCACCGGTGCCTTTTGTTATTCATTTACGGGAATGTAGCACGGTGGTGAGTGAACGTGTAGGTGTGGCGTTTCACGGTGTCGCGGATGGTAAAAATCCGGATGTGCTTTCCGTGGGAGAGGGGCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGA  >  NC_000913/4543810‑4544101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑