Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,586,090 A→C A224A (GCT→GCG)  hsdR ← endonuclease R Type I restriction enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,586,0900AC100.0% 169.0 / NA 47A224A (GCT→GCG) hsdRendonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/19);  total (28/19)

GGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTAAGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAAC  >  NC_000913/4585944‑4586236
                                                                                                                                                  |                                                                                                                                                  
ggACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCt                                                                                                                                                <  2:573754/151‑1 (MQ=255)
    gTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACg                                                                                                                                            <  1:185754/151‑1 (MQ=255)
     tGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGt                                                                                                                                           >  2:201398/1‑151 (MQ=255)
       cgcCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTc                                                                                                                                         <  2:677295/151‑1 (MQ=255)
            ttGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGtt                                                                                                                                    >  1:639156/1‑151 (MQ=255)
               gagaAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCgg                                                                                                                                 >  2:460285/1‑151 (MQ=255)
                    gcgcAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGt                                                                                                                            >  1:820830/1‑151 (MQ=255)
                     cgcAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGtt                                                                                                                           <  2:660009/151‑1 (MQ=255)
                        aGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTg                                                                                                                        >  1:266600/1‑151 (MQ=255)
                          ggTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGcc                                                                                                                      >  2:722763/1‑151 (MQ=255)
                           gTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCa                                                                                                                     <  2:881870/151‑1 (MQ=255)
                           gTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCa                                                                                                                     <  1:545697/151‑1 (MQ=255)
                             tttGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAg                                                                                                                    <  2:790566/150‑1 (MQ=255)
                               tGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTGGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTc                                                                                                                 >  1:454715/1‑151 (MQ=255)
                                  tGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCcgc                                                                                                              >  1:333295/1‑151 (MQ=255)
                                   gTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCcg                                                                                                               <  1:421222/149‑1 (MQ=255)
                                       gCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGttct                                                                                                         <  2:173617/151‑1 (MQ=255)
                                        ccTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGttctt                                                                                                        >  2:346164/1‑151 (MQ=255)
                                         cTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGttcttc                                                                                                       >  1:328404/1‑151 (MQ=255)
                                             cAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCgg                                                                                                   <  2:608491/151‑1 (MQ=255)
                                              aGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGc                                                                                                  <  1:404360/151‑1 (MQ=255)
                                              aGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGc                                                                                                  >  1:396665/1‑151 (MQ=255)
                                                    cTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCtg                                                                                            >  1:732701/1‑151 (MQ=255)
                                                      ttACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCtgtg                                                                                          >  1:582317/1‑151 (MQ=255)
                                                         cGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCt                                                                                        >  2:46228/1‑150 (MQ=255)
                                                          gCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTc                                                                                      <  2:636503/151‑1 (MQ=255)
                                                           cAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTcc                                                                                     <  1:506962/151‑1 (MQ=255)
                                                                        atcaGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGc                                                                        <  1:683125/151‑1 (MQ=255)
                                                                          caGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGa                                                                      <  2:241761/151‑1 (MQ=255)
                                                                                 cGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTggg                                                               >  2:859242/1‑151 (MQ=255)
                                                                                   aCTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGtt                                                             >  1:284418/1‑151 (MQ=255)
                                                                                          tcttcGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCt                                                       <  2:220283/150‑1 (MQ=255)
                                                                                             tcGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCgg                                                   >  1:801946/1‑151 (MQ=255)
                                                                                              cGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGt                                                  <  2:276852/151‑1 (MQ=255)
                                                                                                cTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGtt                                                 >  2:85576/1‑150 (MQ=255)
                                                                                                            gtgCGCTCGATGGCCTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGc                                    >  2:702068/1‑151 (MQ=255)
                                                                                                                         ccTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGc                       >  1:186772/1‑151 (MQ=255)
                                                                                                                         ccTGATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGc                       >  2:123613/1‑151 (MQ=255)
                                                                                                                            gATCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGat                    >  2:901228/1‑151 (MQ=255)
                                                                                                                             aTCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGat                    >  2:709894/1‑150 (MQ=255)
                                                                                                                              tCGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGata                   <  2:254682/150‑1 (MQ=255)
                                                                                                                               cGGTAATTTCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGatat                  >  2:6845/1‑150 (MQ=255)
                                                                                                                                      ttCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGtt           <  1:864049/150‑1 (MQ=255)
                                                                                                                                      ttCTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTc          >  2:191328/1‑151 (MQ=255)
                                                                                                                                        cTTTGTGGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTc          >  2:912959/1‑149 (MQ=255)
                                                                                                                                           tgtgGTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGc     >  2:152331/1‑151 (MQ=255)
                                                                                                                                              ggTACGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAAc  >  2:207299/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTAAGCCTTACGTTCCTGTTCGGTCTGTTTTGCCAGTTCCGCGTTCTTCTCGGCGAGCTGTGCTTCCAGTGCCGCAAGGCGAGCCTGGGTTTGCGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAAC  >  NC_000913/4585944‑4586236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑