Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,153	T→G	I116S (ATC→AGC)	yaaU →	putative MFS sugar transporter; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,153	0	T	G	100.0%	196.1 / NA	51	I116S (ATC→AGC)	yaaU	putative MFS sugar transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/22);  total (29/22)

GGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTG  >  NC_000913/46011‑46297                                                                                                                                               |                                                                                                                                                 ggCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTg                                                                                                                                          >  2:468110/1‑151 (MQ=255)  gCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGt                                                                                                                                         <  2:399676/151‑1 (MQ=255)   cacaTCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTc                                                                                                                                        >  1:151091/1‑151 (MQ=255)      aTCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATc                                                                                                                                     >  2:896428/1‑151 (MQ=255)      aTCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATc                                                                                                                                     <  1:668904/151‑1 (MQ=255)        cGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCgg                                                                                                                                   >  2:590981/1‑151 (MQ=255)        cGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCg                                                                                                                                    <  2:125923/150‑1 (MQ=255)                  ttATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTTATCCCCCGTCGAACTGTTGGGGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTAt                                                                                                                         >  2:812818/1‑151 (MQ=255)                   tataTTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTAt                                                                                                                         <  1:917289/150‑1 (MQ=255)                        ttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATc                                                                                                                   >  2:106936/1‑151 (MQ=255)                        ttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATc                                                                                                                   >  2:497739/1‑151 (MQ=255)                        ttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATc                                                                                                                   >  1:356571/1‑151 (MQ=255)                         tCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATc                                                                                                                   <  1:795733/150‑1 (MQ=255)                             aTAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCAc                                                                                                              >  2:5951/1‑151 (MQ=255)                             aTAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCAc                                                                                                              <  1:439919/151‑1 (MQ=255)                                aaGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTc                                                                                                           >  1:591543/1‑151 (MQ=255)                                aaGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTc                                                                                                           >  1:917621/1‑151 (MQ=255)                                  gTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCaa                                                                                                         <  1:446612/151‑1 (MQ=255)                                    cGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATg                                                                                                       <  1:684948/151‑1 (MQ=255)                                      gACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGAt                                                                                                     >  1:909889/1‑151 (MQ=255)                                        cGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATc                                                                                                    >  2:339499/1‑150 (MQ=255)                                              aaaaTGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAg                                                                                              >  2:558263/1‑150 (MQ=255)                                                   gTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTcc                                                                                        <  2:619059/151‑1 (MQ=255)                                                    ttCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTcc                                                                                        <  2:702194/150‑1 (MQ=255)                                                    ttCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTcc                                                                                        >  2:475331/1‑150 (MQ=255)                                                          aTTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTAccc                                                                                 <  2:663439/151‑1 (MQ=255)                                                          aTTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTAccc                                                                                 >  1:613431/1‑151 (MQ=255)                                                             gATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGGGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGt                                                                               >  1:120125/1‑150 (MQ=255)                                                                   atcGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCggg                                                                        <  1:218953/151‑1 (MQ=255)                                                                     cGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGc                                                                       <  1:777307/150‑1 (MQ=255)                                                                        cATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGttt                                                                   >  1:505081/1‑151 (MQ=255)                                                                         aTCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGtttt                                                                  >  1:11162/1‑151 (MQ=255)                                                                            ggCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCa                                                               <  1:2559/151‑1 (MQ=255)                                                                                tGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATc                                                             >  1:371962/1‑149 (MQ=255)                                                                                      cGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTAt                                                     >  2:254485/1‑151 (MQ=255)                                                                                       ggTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTAtt                                                    <  2:40494/151‑1 (MQ=255)                                                                                               cgaTGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAt                                            >  2:129997/1‑151 (MQ=255)                                                                                               cgaTGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAt                                            <  2:534663/151‑1 (MQ=255)                                                                                                gaTGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAtg                                           >  1:162322/1‑151 (MQ=255)                                                                                                  tgtttgttTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGAtgtg                                         >  2:172074/1‑151 (MQ=255)                                                                                                       gtttCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTAt                                     >  2:51896/1‑150 (MQ=255)                                                                                                          tCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATCATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCg                                 >  1:72513/1‑151 (MQ=255)                                                                                                            aTCCCCCGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGgc                               <  2:204119/151‑1 (MQ=255)                                                                                                                 ccGTCGAACTGTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGAc                          >  2:472010/1‑151 (MQ=255)                                                                                                                     cGAACTGTTGGTGATGCGGGTACTTAGTGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCtgt                      <  1:653284/151‑1 (MQ=255)                                                                                                                           gTTGGTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGAt                >  2:49983/1‑151 (MQ=255)                                                                                                                              ggTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCt              <  2:716230/150‑1 (MQ=255)                                                                                                                               gTGATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTgg            >  2:678320/1‑151 (MQ=255)                                                                                                                                 gATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTc          <  1:475343/151‑1 (MQ=255)                                                                                                                                 gATGCGGGTACTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTc          <  1:833312/151‑1 (MQ=255)                                                                                                                                          aCTTAGCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTg  <  1:794539/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                 GGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTG  >  NC_000913/46011‑46297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑