Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,927	A→C	K338Q (AAA→CAA)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,927	0	A	C	100.0%	202.5 / NA	54	K338Q (AAA→CAA)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/34);  total (20/34)

TAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG  >  NC_000913/534781‑535074                                                                                                                                                   |                                                                                                                                                    tAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGc                                                                                                                                                 >  2:647764/1‑151 (MQ=255)    agaAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCggg                                                                                                                                              <  1:422230/151‑1 (MQ=255)      aaaTACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGgtc                                                                                                                                            <  1:223821/151‑1 (MQ=255)           cacCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTg                                                                                                                                       <  2:637515/151‑1 (MQ=255)                    gcgcAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGt                                                                                                                              <  1:583561/151‑1 (MQ=255)                                 cATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTc                                                                                                                  <  2:754995/150‑1 (MQ=255)                                  atattgatattgAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGc                                                                                                                >  1:474836/1‑151 (MQ=255)                                    attgatattgaGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGcc                                                                                                               <  2:788182/150‑1 (MQ=255)                                    attgatattgaGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCg                                                                                                              >  2:852268/1‑151 (MQ=255)                                    attgatattgaGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCg                                                                                                              >  2:726655/1‑151 (MQ=255)                                          attgaGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGc                                                                                                         <  1:896711/150‑1 (MQ=255)                                           ttgaGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGcc                                                                                                        >  1:774875/1‑150 (MQ=255)                                              aGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCcagc                                                                                                     >  1:380140/1‑150 (MQ=255)                                              aGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCcagc                                                                                                     >  1:851719/1‑150 (MQ=255)                                               gCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCcagc                                                                                                     >  1:242887/1‑149 (MQ=255)                                                ccGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCcagcag                                                                                                   >  1:742649/1‑150 (MQ=255)                                                    cGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTa                                                                                               >  1:226192/1‑150 (MQ=255)                                                     gCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTaa                                                                                              >  2:364042/1‑150 (MQ=255)                                                      cAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAAc                                                                                            >  1:595569/1‑151 (MQ=255)                                                            ggTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACtt                                                                                      <  1:376031/151‑1 (MQ=255)                                                             gTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACttt                                                                                     <  2:460402/151‑1 (MQ=255)                                                               cgcgTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTtgc                                                                                   <  2:802443/151‑1 (MQ=255)                                                                gcgTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTtgct                                                                                  >  2:767146/1‑151 (MQ=255)                                                                  gTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTtgctgc                                                                                >  1:768258/1‑151 (MQ=255)                                                                    gCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTgcgc                                                                              <  2:573409/151‑1 (MQ=255)                                                                             ggATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCAc                                                                     <  2:703239/151‑1 (MQ=255)                                                                               aTCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACtt                                                                   >  2:299314/1‑151 (MQ=255)                                                                                   cGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTc                                                                  <  2:748856/148‑1 (MQ=255)                                                                                   cGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCg                                                                 <  2:444354/149‑1 (MQ=255)                                                                                      tATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAAc                                                            >  2:172406/1‑151 (MQ=255)                                                                                          gTCGCTGATGCTAAAGCGGCGCTGACTCTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTTTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTg                                                         <  1:906186/150‑1 (MQ=255)                                                                                              ctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCTACTTCGACAACGTGCCg                                                      <  2:371185/149‑1 (MQ=255)                                                                                                gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGt                                                    <  1:530315/149‑1 (MQ=255)                                                                                                    cTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAAcc                                              >  1:33591/1‑151 (MQ=255)                                                                                                      aaaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGc                                            <  2:146677/151‑1 (MQ=255)                                                                                                            gcgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAgcgc                                       <  1:568511/150‑1 (MQ=255)                                                                                                              gcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTGCGACAACGTGCCGGTGAAACCGCAGCGCgtg                                    <  1:614618/151‑1 (MQ=255)                                                                                                                   cacTGCTGGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGa                               <  2:73979/151‑1 (MQ=255)                                                                                                                       gctgGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAag                             <  2:923639/149‑1 (MQ=255)                                                                                                                       gctgGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAag                             <  2:109893/149‑1 (MQ=255)                                                                                                                       gctgGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAag                             <  1:722400/149‑1 (MQ=255)                                                                                                                       gctgGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAag                             >  1:686556/1‑149 (MQ=255)                                                                                                                       gctgGTTGAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAag                             <  1:647779/149‑1 (MQ=255)                                                                                                                              gAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAAc                     <  1:363901/150‑1 (MQ=255)                                                                                                                              gAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACa                    <  1:726673/151‑1 (MQ=255)                                                                                                                              gAAGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACa                    <  2:83284/151‑1 (MQ=255)                                                                                                                                aGTGGCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaaa                  <  1:267105/151‑1 (MQ=255)                                                                                                                                   ggCGCAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGc                <  1:645817/150‑1 (MQ=255)                                                                                                                                      gcAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCttt            <  2:608117/151‑1 (MQ=255)                                                                                                                                      gcAGGAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCttt            >  1:904658/1‑151 (MQ=255)                                                                                                                                         ggAGATGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGt         <  1:82331/151‑1 (MQ=255)                                                                                                                                           agaTGCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcg       >  1:536926/1‑151 (MQ=255)                                                                                                                                               gCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAt   <  2:452823/151‑1 (MQ=255)                                                                                                                                               gCAACAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAt   <  1:398227/151‑1 (MQ=255)                                                                                                                                                caacaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtg  >  2:499988/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG  >  NC_000913/534781‑535074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑