| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 629,546 | A→C | T83P (ACA→CCA) | entH → | enterobactin synthesis proofreading thioesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 629,546 | 0 | A | C | 98.1% | 193.2 / ‑4.0 | 53 | T83P (ACA→CCA) | entH | enterobactin synthesis proofreading thioesterase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (33/19); total (33/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GTGCTGGAAGCCGAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTG > NC_000913/629402‑629692 | gtgCTGGAAGCCGAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAAc > 1:8466/1‑151 (MQ=255) aGCCGAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCa < 2:729704/151‑1 (MQ=255) gCCGAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCa > 1:342751/1‑150 (MQ=255) cGAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAaca < 2:675151/151‑1 (MQ=255) cGAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAac > 1:591381/1‑150 (MQ=255) taaaTGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAacac > 2:276018/2‑151 (MQ=255) gAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAacac > 2:789628/1‑151 (MQ=255) gAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAacac > 1:573848/1‑151 (MQ=255) aTGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACAcc > 2:703478/1‑151 (MQ=255) cGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATc > 2:748223/1‑150 (MQ=255) cGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCg > 2:529657/1‑151 (MQ=255) ggTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGc < 1:815787/151‑1 (MQ=255) tGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCg > 1:478473/1‑151 (MQ=255) aTACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGgt > 1:898104/1‑151 (MQ=255) cccGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGgtg < 2:665610/149‑1 (MQ=255) ccGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCt < 2:501466/151‑1 (MQ=255) ccGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCt < 2:390746/151‑1 (MQ=255) cGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTg < 2:607741/151‑1 (MQ=255) aCTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAgg > 2:521372/1‑151 (MQ=255) caGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAgg > 1:914505/1‑151 (MQ=255) caGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAg < 2:418999/150‑1 (MQ=255) aGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGt > 1:9655/1‑151 (MQ=255) cGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTAcg < 1:387458/151‑1 (MQ=255) cGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTAcg > 1:566513/1‑151 (MQ=255) gTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTAcgc < 2:75406/151‑1 (MQ=255) gTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTAcgc < 1:230647/151‑1 (MQ=255) gTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTAcgc > 2:337402/1‑151 (MQ=255) gTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTAcgc > 2:123438/1‑151 (MQ=255) cGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACgcgg < 1:776944/150‑1 (MQ=255) ttACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCt > 1:646068/1‑151 (MQ=255) ttACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCt > 2:312936/1‑151 (MQ=255) tGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCg > 1:208550/1‑151 (MQ=255) gcgcGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGc > 1:886612/1‑150 (MQ=255) cgcgTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCAt < 1:480722/151‑1 (MQ=255) gcgTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATc > 1:479471/1‑151 (MQ=255) cgTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCt > 1:813386/1‑151 (MQ=255) gTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCtt < 1:170414/151‑1 (MQ=255) gTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCtt > 1:283449/1‑151 (MQ=255) gcgcTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGc > 2:163038/1‑151 (MQ=255) gcTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCa > 1:921774/1‑150 (MQ=255) gAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCaga < 2:97330/151‑1 (MQ=255) gggATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATTGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGaa < 1:769012/151‑1 (MQ=255) gCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGttt > 2:394246/1‑151 (MQ=255) gATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCg > 1:264718/1‑150 (MQ=255) aTTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGAt > 1:673651/1‑151 (MQ=255) aTTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGAt < 2:721993/151‑1 (MQ=255) gaCCCGCGACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAgggg > 2:268114/1‑151 (MQ=255) gcgACGGACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGgcggcg > 1:773726/1‑151 (MQ=255) gACAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTtgctg > 1:362754/1‑151 (MQ=255) aCAGTGTGTGGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTtgctgc < 2:226987/151‑1 (MQ=255) gtgtgGTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTg < 1:353149/151‑1 (MQ=255) ggTAGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCg < 1:789646/150‑1 (MQ=255) aGGCCCAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTg > 2:671174/1‑151 (MQ=255) | GTGCTGGAAGCCGAAATGCCGGTTGATACCCGTACTCATCAGCCGTTCGGTTTACTACATGGCGGCGCGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTG > NC_000913/629402‑629692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |