Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,883	T→G	L157V (TTA→GTA)	djlB →	putative HscC co‑chaperone, uncharacterized J domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,883	0	T	G	100.0%	226.5 / NA	58	L157V (TTA→GTA)	djlB	putative HscC co‑chaperone, uncharacterized J domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/33);  total (25/33)

ATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATT  >  NC_000913/677736‑678024                                                                                                                                                    |                                                                                                                                              aTTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGtat                                                                                                                                            <  2:530225/151‑1 (MQ=255)   tgatgaATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGtata                                                                                                                                           <  1:624009/150‑1 (MQ=255)   tgatgaATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGAGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGtata                                                                                                                                           >  1:625694/1‑150 (MQ=255)    gatgaATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTatat                                                                                                                                          <  1:604179/150‑1 (MQ=255)       gaATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCa                                                                                                                                      <  1:241056/151‑1 (MQ=255)            aCGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGc                                                                                                                                  <  1:908666/150‑1 (MQ=255)              gCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGaa                                                                                                                               <  1:540953/151‑1 (MQ=255)                 ggTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAggg                                                                                                                            >  1:28013/1‑151 (MQ=255)                 ggTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAgg                                                                                                                             <  2:399317/150‑1 (MQ=255)                 ggTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAgg                                                                                                                             <  1:506139/150‑1 (MQ=255)                 ggTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAgg                                                                                                                             <  1:199527/150‑1 (MQ=255)                    cGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTAAAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGa                                                                                                                          <  1:487354/150‑1 (MQ=255)                       tgtgCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGt                                                                                                                      >  2:69051/1‑151 (MQ=255)                            acaATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTa                                                                                                                 <  1:717509/151‑1 (MQ=255)                              aaTCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAAc                                                                                                               >  1:317885/1‑151 (MQ=255)                                  gCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTcc                                                                                                           >  2:865349/1‑151 (MQ=255)                                    cATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAg                                                                                                         <  1:827971/151‑1 (MQ=255)                                     aTGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTcc                                                                                                           >  1:827786/1‑148 (MQ=255)                                       gAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                       <  2:450866/150‑1 (MQ=255)                                        aaCACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGAcc                                                                                                      >  2:54507/1‑150 (MQ=255)                                           acTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTc                                                                                                  <  2:918573/151‑1 (MQ=255)                                             tGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTg                                                                                                >  2:35928/1‑151 (MQ=255)                                                    ttATCCTTCGAGTGCGTGGCGTTATTAGCAGAACGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGc                                                                                         >  2:210736/1‑151 (MQ=255)                                                      aTCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGccc                                                                                       >  2:727900/1‑151 (MQ=255)                                                           tCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTg                                                                                   <  2:90728/150‑1 (MQ=255)                                                            cGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGcc                                                                                 >  1:598939/1‑151 (MQ=255)                                                             gAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCg                                                                                >  1:650554/1‑151 (MQ=255)                                                                  cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCaa                                                                           <  1:75589/151‑1 (MQ=255)                                                                       tgtTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATg                                                                       >  2:812256/1‑150 (MQ=255)                                                                           attaGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGacac                                                                   <  1:218359/150‑1 (MQ=255)                                                                                cAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATca                                                              <  2:159741/150‑2 (MQ=255)                                                                                 aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAt                                                            >  1:634455/1‑151 (MQ=255)                                                                                    aaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAtttt                                                         <  1:626728/151‑1 (MQ=255)                                                                                      gATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTa                                                       <  2:867865/151‑1 (MQ=255)                                                                                       aTTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTAc                                                      >  1:919143/1‑151 (MQ=255)                                                                                            ggtggtTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAAt                                                 <  1:552659/151‑1 (MQ=255)                                                                                                  tACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGc                                           >  1:889085/1‑151 (MQ=255)                                                                                                    cAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACTCCATCGATTTTTACCAAATGTTCGCTc                                         <  1:518199/151‑1 (MQ=255)                                                                                                        aggaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGAt                                     <  1:174088/151‑1 (MQ=255)                                                                                                         ggaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGAtt                                    <  2:216287/151‑1 (MQ=255)                                                                                                          gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGAttt                                   <  2:743394/151‑1 (MQ=255)                                                                                                           aaaaTACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTg                                  <  1:370291/151‑1 (MQ=255)                                                                                                            aaaTACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTgg                                 >  1:182900/1‑151 (MQ=255)                                                                                                                      gAAATAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATc                       >  2:668147/1‑151 (MQ=255)                                                                                                                       aaaTAGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATcc                      <  1:54507/151‑1 (MQ=255)                                                                                                                           aGACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCcaca                   >  1:448095/1‑150 (MQ=255)                                                                                                                            gACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAAt                 <  2:98292/151‑1 (MQ=255)                                                                                                                            gACGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAAt                 <  2:672010/151‑1 (MQ=255)                                                                                                                             aCGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATg                <  2:488437/151‑1 (MQ=255)                                                                                                                             aCGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATg                >  2:256454/1‑151 (MQ=255)                                                                                                                              cGAAGAAGAACTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATgg               >  1:200709/1‑151 (MQ=255)                                                                                                                               gaagaagaaCTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc              >  2:36589/1‑151 (MQ=255)                                                                                                                               gaagaagaaCTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc              <  1:791120/151‑1 (MQ=255)                                                                                                                                 agaagaaCTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg            <  1:212774/151‑1 (MQ=255)                                                                                                                                  gaagaaCTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg            >  1:222394/1‑150 (MQ=255)                                                                                                                                      aaCTGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACAtt       <  1:488948/151‑1 (MQ=255)                                                                                                                                         tGGAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGt     >  2:869852/1‑150 (MQ=255)                                                                                                                                           gAATCCTTTGTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTAtt  >  1:287840/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                              ATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATT  >  NC_000913/677736‑678024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑